4.2.1.138 Mg2+ more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2 715629 4.2.1.138 Mn2+ 38% activity (relative to the activity in the presence ofMg2+) is retained in the presence of 5 mM Mn2+ 715629 4.2.1.138 additional information no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+ 715629