3.6.1.52 Co2+ Co2+ supports less than 5% of the activity with Mg2+ 712171 3.6.1.52 Mg2+ 1-2 mM required 393824 3.6.1.52 Mg2+ divalent cation is required, pH 7.2, at 37ºC 659817 3.6.1.52 Mg2+ Mg2+ is the most effective stimulatory cation above 10 mM 712171 3.6.1.52 Mg2+ required, activates 756069 3.6.1.52 Mn2+ divalent cation is required, 3 mM, pH 7.2, at 37ºC, 50% higher activity than Mg2+ at 10 to 15 mM 659817 3.6.1.52 Mn2+ Mn2+ is also effective, with an optimal concentration of 1 mM 712171 3.6.1.52 additional information Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps 712171