3.1.3.B8 Ca2+ divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively 708933 3.1.3.B8 Co2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ 732910 3.1.3.B8 Mg2+ divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively 708933 3.1.3.B8 Mg2+ highest activity with 10 mM Mg2+ 732910 3.1.3.B8 Mg2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+. Maximum activity in presence of 10 mM Mg2+ 732910 3.1.3.B8 Mn2+ divalent cations are not required for activity, but Mn2+, Mg2+, and Ca2+ (each 20 mM) leads to an increase in the activity by 100, 52, and 47%, respectively 708933 3.1.3.B8 additional information NaCl and KCl concentrations between 50 and 300 mM had no effect on the enzyme activity 708933 3.1.3.B8 Ni2+ - 732910 3.1.3.B8 Ni2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ 732910