3.5.1.4 Ba2+ activity enhanced 209106 3.5.1.4 Ca2+ activates at 1 mM 718672 3.5.1.4 Ca2+ activation above 100 mM 655874 3.5.1.4 Co2+ 1 mM, about 20% activation 719432 3.5.1.4 Co2+ increases 1.3fold the activity at 1 mM, inhibition at higher concentration 655874 3.5.1.4 Cobalt 0.33 mol of cobalt per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis 209115 3.5.1.4 Cr2+ activity enhanced 209106 3.5.1.4 Fe3+ 1 mM, about 20% activation 719432 3.5.1.4 Iron 0.33 mol of iron per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis 209115 3.5.1.4 Iron 8 mol of iron per mol of enzyme, detection by inductively coupled plasma-atomic emission spectrometry 209106 3.5.1.4 Mg2+ 10 mM, 1.15fold stimulation 724942 3.5.1.4 Mg2+ activates at 1 mM 718672 3.5.1.4 Mg2+ activation above 100 mM 655874 3.5.1.4 Mg2+ required for enzyme activity 712780 3.5.1.4 Mn2+ activates at 1 mM 718672 3.5.1.4 Mn2+ activation above 100 mM 655874 3.5.1.4 additional information enzyme has no metal ion requirement 209116 3.5.1.4 additional information no effect: Cu2+, Mn2+, and Ca2+ 719432 3.5.1.4 additional information no metalloenzyme, no effect on enzyme activity by Fe2+, Mn2+, Mg2+, and Ca2+ 733127 3.5.1.4 additional information no requirement for metal ions 733190 3.5.1.4 NaCl influences the oligomerization status of the enzyme. 60% octameric and 40% dimeric enzyme in absence of NaCl, 5% octameric and 95% dimeric enzyme at and above 150 mM NaCl, profile overview 711039 3.5.1.4 Ni2+ increases 1.1fold the activity at 1 mM, inhibition at higher concentration 655874 3.5.1.4 V2+ 1 mM, about 20% activation 719432 3.5.1.4 Zn2+ required for activity 655874