3.4.21.4	Ca2+	-	707409, 708498, 708554, 708855, 709189, 710243	
3.4.21.4	Ca2+	1 mM, 114% of initial activity	731682	
3.4.21.4	Ca2+	10 mM Ca2+ have a stabilizing effect	679952	
3.4.21.4	Ca2+	2 mM, stabilizing effect	680305	
3.4.21.4	Ca2+	activates at concentrations above 0.4 mM	678896	
3.4.21.4	Ca2+	activates, best at 20 mM	708221	
3.4.21.4	Ca2+	activates, plays an important role in trypsin stabilization	717901	
3.4.21.4	Ca2+	highest activity in presence of 30-50 mM, isoforms A, B, C	668311	
3.4.21.4	Ca2+	required	717278, 717556	
3.4.21.4	Ca2+	required for thermal stability	669029	
3.4.21.4	Ca2+	required, amino acid residues Glu70, Asn72, Glu77, and Glu80 are conserved in the calcium-binding loop	717554	
3.4.21.4	Ca2+	stabilization of enzyme	668694	
3.4.21.4	Ca2+	stabilized in the presence of 2 mM Ca2+	679341	
3.4.21.4	Ca2+	stabilizes	708552	
3.4.21.4	Ca2+	stabilizes the enzyme	708529	
3.4.21.4	Ca2+	stabilizes the purified enzyme	708835	
3.4.21.4	additional information	Ca2+, Mg2+, K+, and Na+ do not affect the enzyme's proteolytic activity	717612	
3.4.21.4	additional information	no activation by Ca2+	650887	
3.4.21.4	additional information	not dependent on Ca2+ for activation or stability	699194	
3.4.21.4	Na+	Na+ activation is interwoven with substrate selectivity in the trypsin scaffold	699531	
3.4.21.4	Ni2+	trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency	95451	
3.4.21.4	Zn2+	trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency	95451