3.1.31.1	Ca2+	-	697993	
3.1.31.1	Ca2+	1 mM, unfolding forces in the presence of calcium ion are slightly increased	696005	
3.1.31.1	Ca2+	30 mM CaCl2 are included in assay medium	705843	
3.1.31.1	Ca2+	5 mM CaCl2 are included in assay medium	705843	
3.1.31.1	Ca2+	activates to 120% of initial activity at 0.05 mM	730338	
3.1.31.1	Ca2+	activity is Ca2+ dependent, 10 mM are included in assay buffer	705421	
3.1.31.1	Ca2+	calcium-dependent nuclease activity	729764	
3.1.31.1	Ca2+	dependent on	752163	
3.1.31.1	Ca2+	enzyme is Ca2+ dependent for its activity	702789	
3.1.31.1	Ca2+	hydrolysis of DNA and RNA is completely dependent on Ca2+, KM for wild-type enzyme is 0.113 mM	664247	
3.1.31.1	Ca2+	required	135023, 135024, 135028, 135031, 135032, 135037, 680252, 717382, 717951	
3.1.31.1	Ca2+	the carboxylic groups in the active site of SNase are known to bind Ca2+	706658	
3.1.31.1	Co2+	activates slightly at 5 mM	730338	
3.1.31.1	Cu2+	minimal activation if Ca2+ is replaced by Cu2+	135028	
3.1.31.1	Fe2+	minimal activation if Ca2+ is replaced by Fe2+	135028	
3.1.31.1	Mg2+	activates to 120% of initial activity at 0.5 mM	730338	
3.1.31.1	Mg2+	required	717951	
3.1.31.1	additional information	examination of salt sensitivity of pka-values (chemical shifts analyzed by NMR while titrating) His8, His46, His121 and His124. Tested variants D21N/T33V/T41I/S59A/P117G/A128A mutant, E75A mutant, E75Q mutant, and E101A mutant in 0.01 M, 0.10 M, and 1.0 M KCl	699534	
3.1.31.1	additional information	isozyme Nuc1 only exhibits a very small increase in thermonuclease activity in the presence of Ca2+ (0.05 mM), Mg2+ (0.05 mM), Co2+ (0.5 mM) and Ni2+ (0.05 mM)	730338	
3.1.31.1	additional information	isozyme Nuc2 activity is cation-dependent	730786	
3.1.31.1	additional information	the enzyme activity is not affected by Mg2+ and Mn2+	729764	
3.1.31.1	Ni2+	activates slightly at 0.05 mM	730338	
3.1.31.1	Sr2+	DNase but no RNase activity if Ca2+ is replaced Sr2+	135028