2.7.1.32 Ca2+ required 673201 2.7.1.32 Carpronium chloride stimulates 641359, 641371 2.7.1.32 Chlorocholine chloride stimulates 641359, 641371 2.7.1.32 K+ KCl activates 641357 2.7.1.32 K+ stimulates 641359, 641371 2.7.1.32 Li+ stimulates 641359, 641371 2.7.1.32 Mg2+ - 662203, 671362 2.7.1.32 Mg2+ 10 mM MgSO4 required for maximal activity 641357 2.7.1.32 Mg2+ 11 mM used in assay conditions 760908 2.7.1.32 Mg2+ ATP concentration 1.25-2.5 mM: reaction requires free Mg2+ rather than MgATP2-, ATP concentration exceeding that of Mg2+: strong inhibition 641345 2.7.1.32 Mg2+ dependent on 739407 2.7.1.32 Mg2+ Km MgATP2-: 10 mM, in presence of equimolar amounts of ATP and Mg2+, Km MgATP2-: 1.5 mM, in 2.5 fold higher concentration of Mg2+ than ATP 641345 2.7.1.32 Mg2+ maximal activity at equimolar concentration of ATP and Mg2+ 641359, 641371 2.7.1.32 Mg2+ Mg2+:ATP ratio for optimal activity is 30:10 mM 641349 2.7.1.32 Mg2+ MgATP2- is the true substrate 641359 2.7.1.32 Mg2+ optimal stimulation at 13 mM in the presence of 10 mM ATP 641364 2.7.1.32 Mg2+ required 641351, 641358, 641366, 641370, 641371, 641372, 641375, 641377, 641380, 663273, 663274, 673201, 721292, 722672, 739680, 758809, 759237, 759595, 759713, 759857, 760133, 760183 2.7.1.32 Mg2+ required for activity 672371 2.7.1.32 Mg2+ required, enzyme binding structure analysis. The ADP molecule also participates in the coordination of two magnesium ions 759092 2.7.1.32 Mg2+ requires free Mg2+ as well as MgATP2- 641364 2.7.1.32 Mg2+ stimulates most efficiently of divalent cations 641359, 641371 2.7.1.32 MgCl2 assay at 671383 2.7.1.32 Mn2+ - 641366 2.7.1.32 Mn2+ cannot substitute for Mg2+ 641364 2.7.1.32 Mn2+ one-sixth as effective as Mg2+ 641359, 641371 2.7.1.32 Mn2+ poor substitute for Mg2+ 641375 2.7.1.32 additional information Co2+, Cd2+: almost completely ineffective, Ca2+, Ni2+, Zn2+: totally ineffective 641359, 641371 2.7.1.32 Na+ 8.7-35 mM: slight activation, 70 mM: inactivation 641362 2.7.1.32 Na+ NaCl activates 641357 2.7.1.32 Na+ stimulates 641359, 641371 2.7.1.32 NH4+ stimulates 641359, 641371