2.3.1.101	(NH4)2SO4	-	486087	
2.3.1.101	(NH4)2SO4	1 M increases the activity 20fold	486095	
2.3.1.101	(NH4)2SO4	can substitute for K2HPO4 in stimulation	486086	
2.3.1.101	K+	potassium cyclic 2,3-diphosphoglycerate required for activity at 1 M, can be substituted by other salts with strongly hydrated anions, especially by K+, the enzyme is inactive and thermolabile, and changes the oligomerization state at low salt concentrations	661043	
2.3.1.101	K2HPO4	1.5 M stimulates 10fold	486095	
2.3.1.101	K2HPO4	1.5 M stimulates 3.8fold	486086	
2.3.1.101	K2HPO4	no activity in absence, assay mixture contains 2 M	486086, 486087	
2.3.1.101	K3PO4	0.01 M salt concentration is required for 50% activity at 37°C. 0.1 M salt concentration is required for 100% activity at 37°C	726443	
2.3.1.101	K3PO4	0.1 M salt concentration is required for 50% activity at 83°C. 1.0 M salt concentration is required for 100% activity at 83°C	726443	
2.3.1.101	K3PO4	1.0 M salt concentration is required for 50% activity at 89°C. 1.5 M salt concentration is required for 100% activity at 89°C	726443	
2.3.1.101	additional information	not: Fe, Mo, V, Wo	486095	
2.3.1.101	NaCl	1 M increases the activity 20fold	486095	
2.3.1.101	potassium phosphate	required for activity at 1 M, can be substituted by other salts with strongly hydrated anions, especially by potassium cyclic 2,3-diphosphoglycerate, the enzyme is inactive and thermolabile, and changes the oligomerization state at low salt concentrations	661043	
2.3.1.101	salts	absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl	486087	
2.3.1.101	salts	optimal salt concentration for activity: 1.5-2.0 M	486091	
2.3.1.101	salts	stimulation of activity by salt 1000fold	486097