1.14.13.128 Ca2+ 1 mM, activation to 123% of control 1.14.13.128 Fe2+ preincubation of Ndm for 15 min with 1 mM Fe2+, followed by desalting, increases the iron content to 20.1 mol per mol hexameric N-demethylase component (Ndm) of the N-demethylase holoenzyme. After this treatment, Ndm specific activity increases about 6fold (when 50 mM Fe2+ is present in the enzyme reaction mixture). N-Demethylase component (Ndm) of the N-demethylase holoenzyme is deduced to be a Rieske [2Fe-2S]-domain containing non-haem iron oxygenase 1.14.13.128 Fe2+ NdmA enzyme is a Rieske (2Fe-2S) non-heme iron monooxygenase 1.14.13.128 Iron a Rieske nonheme iron monooxygenase. The enzyme contains 2 mol of iron per mol of monomer 1.14.13.128 Iron contains a non-heme iron domain 1.14.13.128 Mg2+ 1 mM, activation to 132% of control