1.13.11.33	Ca2+	-	639258	
1.13.11.33	Ca2+	1 mM	672376	
1.13.11.33	Ca2+	50% of maximal activity at 0.1 mM Ca2+ in presence of 5 mM EGTA	639250	
1.13.11.33	Ca2+	activates reaction with crude extract	639253	
1.13.11.33	Ca2+	assay in presence of Ca2+	702247	
1.13.11.33	Ca2+	at 0.005-0.05 mM is required for efficient membrane binding	659378	
1.13.11.33	Ca2+	Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane	741942	
1.13.11.33	Ca2+	enhances activity, reversible by EGTA, inhibited by phosphatidyl serine and phosphatidyl choline	639241	
1.13.11.33	Ca2+	for maximal intracellular activity, 12/15-lipoxygenases require a rise in cytosolic calcium concentration inducing a translocation of the enzyme from the cytosol to cellular membranes	639258	
1.13.11.33	Ca2+	in the presence of Ca2+, with or without calcium ionophore, the majority of 15-LO-1 is found in the membrane fraction, although significant amounts are also detected in the supernatant fraction	703628	
1.13.11.33	Ca2+	optimal activity at 2 mM or above	639249	
1.13.11.33	Fe	-	702435, 702550, 706032	
1.13.11.33	Fe	iron-containing metalloenzyme	702344	
1.13.11.33	Fe2+	a non-heme iron in the active side	742000	
1.13.11.33	Fe2+	a non-heme iron-containing dioxygenase	741942	
1.13.11.33	Fe2+	addition in a molar excess, more than 50:1 purified protein:Fe2+, leads to a 3fold increase in the specific activity	639261	
1.13.11.33	Fe2+	enzyme-bound, required for catalysis	742613	
1.13.11.33	Fe2+	iron-containing metalloenzyme	702324	
1.13.11.33	Fe2+	LOX2 contains iron with an iron to protein ratio of 45%	712421	
1.13.11.33	Fe2+	required for the catalytic cycle of fatty acid oxidation by lipoxygenase, overview	685265	
1.13.11.33	Fe2+	required, iron content of the 15-hLO isozymes determined with a Finnigan inductively coupled plasma mass spectrometer, ICP-MS, using cobalt-EDTA as an internal standard	685266	
1.13.11.33	Iron	-	639237	
1.13.11.33	Iron	contains 0.95 mol of iron per mol of enzyme	639259	
1.13.11.33	Iron	contains 1 mol of non-heme iron per mol of enzyme	639257	
1.13.11.33	Iron	contains 1.76 mol of iron per mol of enzyme	639251	
1.13.11.33	Iron	contains iron	639237	
1.13.11.33	Iron	nonheme iron dioxygenase	639264	
1.13.11.33	Iron	the iron is liganded by four histidines at positions 361, 366, 541 and 545 asell as the C-terminal isoleucine	639266	
1.13.11.33	Iron	the recombinant enzyme contains a variable amount of iron, often as low as 0.2 mol per mol of enzyme	639261	
1.13.11.33	Mg2+	activates reaction with crude extract	639253	
1.13.11.33	Mg2+	in the presence of Mg2+, with or without calcium ionophore, the majority of 15-LO-1 is found in the membrane fraction, although significant amounts are also detected in the supernatant fraction	703628	
1.13.11.33	Mn2+	activates reaction with crude extract	639253