1.13.11.3 2,2'-dipyridyl activates the enzyme immobilized on calcium alginate, but inhibits the free enzyme and the enzyme immobilized on glyoxyl agarose, at 0.3 mM 742046 1.13.11.3 Al3+ activates the free enzyme and enzyme immobilized on calcium alginate 742046 1.13.11.3 Cu2+ increases enzyme activity for both cell-free and immobilized extracts by 8% and 44%, respectively 726481 1.13.11.3 EDTA activates the enzyme immobilized on calcium alginate, but inhibits the free enzyme and the enzyme immobilized on glyoxyl agarose, at 0.3 mM 742046 1.13.11.3 Fe - 665455 1.13.11.3 Fe 1.3-1.5 g-atom Fe3+ per mol of enzyme 439499 1.13.11.3 Fe 10 g-atoms per mol enzyme 439501 1.13.11.3 Fe 5 Fe atoms per holoenzyme 439503 1.13.11.3 Fe 5 irons per enzyme molecule 439484 1.13.11.3 Fe 6.6 iron atoms per holoenzyme of 700000 Da 439493 1.13.11.3 Fe 7.27 g-atoms iron per mol enzyme 439500 1.13.11.3 Fe enzyme contains 4 Fe3+ 439504 1.13.11.3 Fe enzyme contains 7.3 g-atom of nonheme iron per mol of enzyme 439483 1.13.11.3 Fe external addition of FeSO4 is absolutely essential, other metal ions cannot replace Fe2+ 439488 1.13.11.3 Fe2+ activates by 29% at 3 mM 726535 1.13.11.3 Fe3+ 115% activity at 1 mM 741660 1.13.11.3 Fe3+ activates the enzyme immobilized on calcium alginate 742046 1.13.11.3 Fe3+ Fe(III)-containing enzyme, 0.8 mol of iron per subunit 763959 1.13.11.3 Fe3+ increases enzyme activity for both cell-free and immobilized extracts by 16% and 99%, respectively 726481 1.13.11.3 Fe3+ presence of a alphabetaFe3+ protomer in a variety of stoichiometries 657551 1.13.11.3 additional information protective effect of immobilization on calcium alginate on enzyme activity is observed in the presence of Cd2+, Al3+, and Fe3+ while in the presence of Mn2+ and Zn2+ the inhibitory effect of metals on enzyme activity increases 742046