1.13.11.15 additional information - additional information Michaelis-Menten steady-state kinetics under air-saturated conditions. The rate determining steps of the PaDHPAO reaction at temperatures above and below 35°C may be different 743618 1.13.11.15 0.0004 - 3,4-Dihydroxyphenylacetate 24°C, pH 7.5, mutant enzyme H200E 672032 1.13.11.15 0.0034 - 3,4-Dihydroxyphenylacetate 32°C 439618 1.13.11.15 0.0037 - 3,4-Dihydroxyphenylpropionic acid 32°C 439618 1.13.11.15 0.004 - 3,4-Dihydroxyphenylacetate 28°C 439618 1.13.11.15 0.0047 - 3,4-Dihydroxyphenylacetate 21°C 439618 1.13.11.15 0.005 - 3,4-Dihydroxyphenylacetate 24°C, pH 7.5, mutant enzyme H200A 672032 1.13.11.15 0.0058 - 3,4-Dihydroxyphenylacetate 38°C 439618 1.13.11.15 0.0074 - 3,4-Dihydroxyphenylacetate 47°C 439618 1.13.11.15 0.01 - 3,4-Dihydroxyphenylacetate 24°C, pH 7.5, mutant enzyme H20N 672032