3.2.1.B41 additional information - 4-nitrophenyl beta-D-glucopyranoside Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C 748250 3.2.1.B41 additional information - additional information when the kinetics are performed below 60°C, the enzyme is inhibited by high substrate concentrations. At temperatures higher than 60°C, the inhibition phenomenon is no longer observed but, on the contrary, an activation is obtained at high substrate concentrations. Around 60°C, the enzyme displays Michaelian behavior 747918 3.2.1.B41 0.08 - 4-nitrophenyl beta-D-fucopyranoside 80°C, pH 8.5 748250 3.2.1.B41 0.1 - 4-nitrophenyl beta-D-glucoside 60°C, pH 7.0 747918 3.2.1.B41 0.12 - 4-nitrophenyl beta-D-fucoside 60°C, pH 7.0 747918 3.2.1.B41 0.16 - 4-nitrophenyl beta-D-glucopyranoside 80°C, pH 8.5 748250 3.2.1.B41 0.28 - 4-nitrophenyl beta-D-glucopyranoside 70°C, pH 8.5 748250 3.2.1.B41 0.39 - 4-nitrophenyl beta-D-glucopyranoside 60°C, pH 8.5 748250 3.2.1.B41 0.83 - 4-nitrophenyl beta-D-glucopyranoside 50°C, pH 8.5 748250 3.2.1.B41 1.74 - 4-nitrophenyl beta-D-galactopyranoside 80°C, pH 8.5 748250