6.3.4.14 additional information - additional information - 654566, 654575, 656008 6.3.4.14 additional information - additional information kinetic data 656207 6.3.4.14 additional information - additional information ordered kinetic mechanism 654403 6.3.4.14 0.00126 - ADP - 1420 6.3.4.14 0.0025 - ATP - 1420 6.3.4.14 0.0076 - ATP - 1420 6.3.4.14 0.0398 - ADP - 1420 6.3.4.14 0.0546 - ATP - 1420 6.3.4.14 0.08 - ADP pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 654566 6.3.4.14 0.081 - ATP 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin 654575 6.3.4.14 0.093 - ATP biotin-dependent ATP hydrolysis, mutant enzyme E23R 675923 6.3.4.14 0.0948 - ATP biotin-dependent ATP hydrolysis, mutant enzyme R19E 675923 6.3.4.14 0.1 2 ATP mutant enzyme R16E, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C 727879 6.3.4.14 0.1 2 ATP wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C 727879 6.3.4.14 0.1049 - ATP biotin-dependent ATP hydrolysis, mutant enzyme F363A 675923 6.3.4.14 0.11 - ATP - 1421, 1428 6.3.4.14 0.1152 - ATP biotin-dependent ATP hydrolysis, wild-type enzyme 675923 6.3.4.14 0.16 - biotin-carboxyl-carrier protein C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein 655973 6.3.4.14 0.17 - ATP wild-type BC 655535 6.3.4.14 0.18 - ADP pH 8, 25°C, E211A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 654566 6.3.4.14 0.19 - ADP pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 656008 6.3.4.14 0.23 - ADP pH 8, 25°C, E288A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 654566 6.3.4.14 0.37 - HCO3- 25°C, wild-type enzyme, bicarbonate-dependent ATP hydrolysis in the absence of biotin 654575 6.3.4.14 0.4 - ADP pH 8, 25°C, N290A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 654566 6.3.4.14 0.5 - HCO3- mutant G165V/G166V, pH 8.0, 25°C 706634 6.3.4.14 0.51 - Carbamoyl phosphate pH 8, 25°C, E211A mutant, ATP synthesis reaction with ADP as cosubstrate 654566 6.3.4.14 0.7 - HCO3- 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin 654575 6.3.4.14 0.8 - HCO3- mutant G166V, pH 8.0, 25°C 706634 6.3.4.14 0.83 - ADP pH 8, 25°C, R292A mutant, ATP synthesis reaction with carbamoyl phosphate as cosubstrate 654566 6.3.4.14 1.7 - HCO3- mutant G165V, pH 8.0, 25°C 706634 6.3.4.14 1.8 - HCO3- wild-type BC 655535 6.3.4.14 1.9 - HCO3- wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C 727879 6.3.4.14 2.3 - Carbamoyl phosphate pH 8, 25°C, E288A mutant, ATP synthesis reaction with ADP as cosubstrate 654566 6.3.4.14 2.8 - Carbamoyl phosphate pH 8, 25°C, R292A mutant, ATP synthesis reaction with ADP as cosubstrate 654566 6.3.4.14 2.9 - HCO3- - 1421, 1428 6.3.4.14 3.3 - ATP mutant G165V, pH 8.0, 25°C 706634 6.3.4.14 3.4 - ATP mutant G166V, pH 8.0, 25°C 706634 6.3.4.14 3.7 - HCO3- K238A mutant 655535 6.3.4.14 3.8 - ATP mutant G165V/G166V, pH 8.0, 25°C 706634 6.3.4.14 3.9 - HCO3- K238Q mutant 655535 6.3.4.14 4.23 - ATP 25°C, C230A mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin 654575 6.3.4.14 4.8 - Carbamoyl phosphate pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with ADP as cosubstrate 656008 6.3.4.14 5 - HCO3- wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C 727879 6.3.4.14 6.5 - HCO3- wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C 727879 6.3.4.14 6.8 - HCO3- K238R mutant 655535 6.3.4.14 6.87 - ATP 25°C, K238Q mutant, bicarbonate-dependent ATP hydrolysis in the absence of biotin 654575 6.3.4.14 8.6 - ATP K238Q mutant 655535 6.3.4.14 11.2 - Carbamoyl phosphate pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with ADP as cosubstrate 654566 6.3.4.14 11.4 - HCO3- wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C 727879 6.3.4.14 13 - ATP K238R mutant 655535 6.3.4.14 16.2 - HCO3- pH 8.5, wild-type 704465 6.3.4.14 18.9 - HCO3- pH 8.5, mutant R338A 704465 6.3.4.14 21 - ATP K238A mutant 655535 6.3.4.14 22.7 - biotin pH 7.4, mutant E296A 704465 6.3.4.14 25 - biotin R338S mutant 656207 6.3.4.14 33.6 - biotin pH 8, 25°C, E211A mutant 654566 6.3.4.14 35.1 - biotin pH 7.4 wild-type 704465 6.3.4.14 41.8 - biotin pH 7.4, mutant R338A 704465 6.3.4.14 50.9 - biotin recombinant biotin carboxylase domain of pyruvate carboxylase 651171 6.3.4.14 56 - biotin M169K mutant 656207 6.3.4.14 57.5 - HCO3- pH 8.5, mutant E296A 704465 6.3.4.14 60.1 - biotin N290A mutant, homodimer 656018 6.3.4.14 60.1 - biotin pH 8, 25°C, N290A mutant 654566 6.3.4.14 62.2 - CO2 recombinant biotin carboxylase domain of pyruvate carboxylase 651171 6.3.4.14 64 - biotin pH 8, 25°C, hybrid dimer K238Q/WT 656018 6.3.4.14 67.6 - biotin pH 8, 25°C, E288A mutant 654566 6.3.4.14 84.8 - biotin free biotin 655973 6.3.4.14 122 - biotin 25°C, free biotin, wild-type enzyme 654575 6.3.4.14 123.6 - biotin pH 8, 25°C, R292A mutant 654566 6.3.4.14 123.6 - biotin R292A mutant, homodimer 656018 6.3.4.14 125 - biotin pH 8, 25°C, K159Q mutant 656008 6.3.4.14 134 - biotin - 654403 6.3.4.14 134 - biotin pH 8, 25°C, wild-type enzyme 654566 6.3.4.14 134 - biotin wild-type enzyme 656008, 656207 6.3.4.14 137 - biotin pH 8, 25°C, E276Q mutant 656008 6.3.4.14 140 - biotin K238Q mutant 655535 6.3.4.14 143 - biotin R338Q mutant 656207 6.3.4.14 147 - biotin pH 8, 25°C, K116A mutant 656008 6.3.4.14 160 - biotin wild-type BC 655535 6.3.4.14 167 - biotin 25°C, free biotin, C230A mutant 654575 6.3.4.14 170 - D-(+)-biotin - 1421, 1428 6.3.4.14 180 - biotin K238A and K238R mutant 655535 6.3.4.14 186 - biotin mutant G166V, pH 8.0, 25°C 706634 6.3.4.14 192.7 - biotin pH 8, 25°C, wild-type enzyme, homodimer 656018 6.3.4.14 256 - biotin mutant G165V, pH 8.0, 25°C 706634 6.3.4.14 321 - biotin mutant G165V/G166V, pH 8.0, 25°C 706634 6.3.4.14 1234 - biotin pH 8, 25°C, H209A mutant 656008