3.8.1.8 additional information - additional information kinetic parameters of diverse mutants relative to the wild-type values, overview 733315 3.8.1.8 additional information - additional information reduction in Km observed in the AtzA variants has allowed the full kinetic profile for the AtzA-catalyzed dechlorination of atrazine to be determined for the first time, revealing the hitherto-unreported substrate cooperativity in AtzA 690560 3.8.1.8 0.02 - 2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine deamination 687391 3.8.1.8 0.02 - hydroxyatrazine - 687391 3.8.1.8 0.0238 - Atrazine - 687101 3.8.1.8 0.029 - 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine deamination 687391 3.8.1.8 0.04 - 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine deamination 687391 3.8.1.8 0.049 - Atrazine A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.049 - Atrazine T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.062 - Atrazine A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.062 - Atrazine A216G/T217D/T219G/A220H/D250D, site-directed mutagenesis, AtzA-variant 734, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.067 - Atrazine A216S/T217A/T219P/A220F/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.067 - Atrazine A216S/T217A/T219P/A220F/D250G, site-directed mutagenesis, AtzA-variant 357, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.076 - Atrazine A216A/T217S/T219H/A220G/D250Y, site-directed mutagenesis, AtzA-variant 431, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.076 - Atrazine T217S/T219H/A220G/D250Y, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.09 - Atrazine A216G/T217D/T219G/A220H/D250G, site-directed mutagenesis, AtzA-variant 430, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.09 - Atrazine Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C 756217 3.8.1.8 0.092 - Atrazine A216S/T217D/T219V/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.092 - Atrazine A216S/T217D/T219V/A220H/D250G, site-directed mutagenesis, AtzA-variant 297, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.092 - Atrazine A216Y/T217D/T219Y/A220H/D250V, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.092 - Atrazine A216Y/T217D/T219Y/A220H/D250V, site-directed mutagenesis, AtzA-variant 422, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.093 - Atrazine A216Y/T217D/A220H/D250E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.093 - Atrazine A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.095 - Atrazine A216G/T217D/T219A/A220V/D250W, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.095 - Atrazine A216G/T217D/T219A/A220V/D250W, site-directed mutagenesis, AtzA-variant 305, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.1 - Atrazine A216H/T217A/T219E/A220S/D250S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.1 - Atrazine A216H/T217A/T219E/A220S/D250S, site-directed mutagenesis, AtzA-variant 662, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.105 - Atrazine A216S/T217D/T219G/A220S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.105 - Atrazine A216S/T217D/T219G/A220S/D250D, site-directed mutagenesis, AtzA-variant 841, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.11 - Atrazine D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C 756217 3.8.1.8 0.12 - 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine dechlorination 687391 3.8.1.8 0.131 - Atrazine Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C 756217 3.8.1.8 0.132 - Atrazine V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C 756217 3.8.1.8 0.138 - Atrazine D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C 756217 3.8.1.8 0.14 - Atrazine V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C 756217 3.8.1.8 0.142 - Atrazine V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C 756217 3.8.1.8 0.145 - Atrazine V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C 756217 3.8.1.8 0.146 - Atrazine recombinant wild-type enzyme, pH 7.2, 25°C 733315 3.8.1.8 0.148 - Atrazine D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C 756217 3.8.1.8 0.149 - Atrazine - 247103 3.8.1.8 0.15 - Atrazine V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C 756217 3.8.1.8 0.15 - Atrazine wild-type enzyme, pH 7.2, 23°C 756217 3.8.1.8 0.153 - Atrazine AtzA wild-type, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 0.153 - Atrazine more than 0.153 mM. AtzA wild type, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme 690560 3.8.1.8 0.23 - 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine dechlorination 687391 3.8.1.8 0.9 - Atrazine A216G/T217D/T219G/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm 690560 3.8.1.8 3.1 - Atrazine mutant E241Q, pH and temperature not specified in the publication 719887 3.8.1.8 3.9 - ametryn wild-type enzyme, pH 7.0, temperature not specified in the publication 719887 3.8.1.8 7.8 - ametryn mutant R325, pH and temperature not specified in the publicationS 719887 3.8.1.8 13.3 - Atrazine mutant R325S, pH and temperature not specified in the publication 719887 3.8.1.8 19 - Atrazine wild-type enzyme, pH 7.0, temperature not specified in the publication 719887