3.6.1.25 additional information - additional information no change in activity after nitrosylation of the enzyme with 250 microM nitrosylated glutathione 669193 3.6.1.25 0.02 - tripolyphosphate wildtype, 2 mM substrate 669193 3.6.1.25 0.021 - tripolyphosphate His-tagged wild type enzyme, at pH 9.7 and 37°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.04 - tripolyphosphate His-tagged wild type enzyme, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.058 - tripolyphosphate His-tagged wild type enzyme, at pH 7.1 and 37°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.078 - tripolyphosphate R265H mutant, 2 mM substrate in the presence of 5 mM ATP 669193 3.6.1.25 0.083 - tripolyphosphate R265H mutant, 2 mM substrate 669193 3.6.1.25 0.1 - tripolyphosphate untagged wild type enzyme, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.122 - tripolyphosphate R265S mutant, 2 mM substrate 669193 3.6.1.25 0.143 - tripolyphosphate R265H mutant, 2 mM substrate in the presence of 5 mM diphosphate 669193 3.6.1.25 0.191 - tripolyphosphate His-tagged mutant enzyme K52R, at pH 9.7 and 37°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.39 - tripolyphosphate His-tagged mutant enzyme K8A, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.72 - tripolyphosphate His-tagged mutant enzyme K85A, at pH 9.7 and 50°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 0.8 - ATP His-tagged wild type enzyme, at pH 8.1 and 50°C, in the presence of 10 mM Mn2+ 719965 3.6.1.25 1.2 - ATP His-tagged mutant enzyme K8A, at pH 10.1 and 50°C, in the presence of 10 mM Mn2+ 719965 3.6.1.25 2.6 - tripolyphosphate His-tagged mutant enzyme K85A, at pH 9.7 and 37°C, in the presence of 5 mM Mg2+ 719965 3.6.1.25 74 - tripolyphosphate His-tagged mutant enzyme K8A, at pH 9.7 and 37°C, in the presence of 5 mM Mg2+ 719965