3.5.1.98 additional information - additional information enzyme kinetic calculations, overview 733081 3.5.1.98 additional information - additional information Km value according to commercial HDAC Fluorescent Activity Assay Kit is 0.143 mM 677978 3.5.1.98 additional information - additional information steady-state kinetics 756690 3.5.1.98 0.000015 - Fluor de Lys mt H611A, pH 7.2, 23°C 677911 3.5.1.98 0.00069 - Ac-IHK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.00228 - Ac-FEK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0024 - Fluor de Lys catalytic domain II, pH 7.2, 23°C 677911 3.5.1.98 0.00244 - Ac-DQK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.00303 - Ac-LIK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0033 - Fluor de Lys wild-type, pH 7.2, 23°C 677911 3.5.1.98 0.0038 - Fluor de Lys mutant H216A, pH 7.2, 23°C 677911 3.5.1.98 0.00405 - Ac-GSK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.0043 - Ac-GGK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0044 - Ac-KYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.0046 - Ac-FEK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.005 - Ac-KWK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0054 - Ac-KGK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.0056 - Ac-KYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0056 - TYSRGGK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.00625 - Ac-IHK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0069 - Ac-LYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.00745 - Ac-KYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.0091 - Ac-IHK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.01 - Ac-GGK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.01 - Ac-PFK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.0104 - YSRGGK(Ac)FFRRF pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.0108 - Ac-GSK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.011 - Ac-GSK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.0117 - Ac-FEK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0119 - Ac-VLK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.0122 - SRGGK(Ac)FFRR pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.0123 - Ac-GS(PO3)K(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0124 - Ac-ILK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.0128 - RGGK(Ac)FFR pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.013 - Fluor de Lys HDAC substrate mutant H843Y 698743 3.5.1.98 0.0133 - Ac-GSK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0138 - Ac-GGK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0148 - Ac-IHK(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.015 - Ac-VLK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.016 - Fluor de Lys HDAC substrate mutant H843A 698743 3.5.1.98 0.016 - Fluor de Lys HDAC substrate mutant H843F 698743 3.5.1.98 0.01646 - Ac-DQK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.017 - Fluor de Lys HDAC substrate wild type enzyme 698743 3.5.1.98 0.019 - Ac-KSK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.0195 - RYSSRRK(Ac)ARPYML pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.021 - SQSEEEK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.0221 - Ac-VLK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0241 - Ac-GSK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.0276 - Ac-GS(PO3)K(acetyl)-7-amido-4-methylcoumarin isoform HDAC3 677964 3.5.1.98 0.0294 - Ac-LYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.03 - Ac-Leu-Gly-Lys(Ac)-7-amido-4-methylcoumarin apparent KM 699458 3.5.1.98 0.03 - Ac-LYK(acetyl)-7-amido-4-methylcoumarin isoform HDAC6 677964 3.5.1.98 0.03 - benzyloxycarbonyl-L-Lys(trifluoroacetyl)-7-amido-4-methylcoumarin isoform HDAC8, 37°C, pH not specified in the publication 719095 3.5.1.98 0.0351 - GGK(Ac)FF pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.0353 - Ac-FEK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.0359 - Ac-FEK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.0391 - Ac-GGK(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.046 - Ac-GS(PO3)K(acetyl)-7-amido-4-methylcoumarin isoform HDAC1 677964 3.5.1.98 0.0489 - Ac-GS(PO3)K(acetyl)-7-amido-4-methylcoumarin isoform HDAC8 677964 3.5.1.98 0.0517 - GK(Ac)F pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.061 - benzyloxycarbonyl-L-Lys(trifluoroacetyl)-7-amido-4-methylcoumarin isoform HDAC7, 37°C, pH not specified in the publication 719095 3.5.1.98 0.066 - Fluor de Lys HDAC substrate mutant F150A 699443 3.5.1.98 0.0666 - Ac-GS(PO3)K(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.0687 - TYSRGGK(Ac)KSEESE pH 7.4, 37°C, recombinant enzyme 756690 3.5.1.98 0.078 - benzyloxycarbonyl-L-Lys(acetyl)-7-amido-4-methylcoumarin isoform HDAC1, 37°C, pH not specified in the publication 719095 3.5.1.98 0.0877 - Ac-GGK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.088 - Fluor de Lys HDAC substrate wild type 699443 3.5.1.98 0.099 - benzyloxycarbonyl-L-Lys(acetyl)-7-amido-4-methylcoumarin isoform HDAC6, 37°C, pH not specified in the publication 719095 3.5.1.98 0.107 - Ac-KYK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.108 - Ac-IHK(acetyl)-7-amido-4-methylcoumarin isoform FB188 HDAH 677964 3.5.1.98 0.14 - Fluor de Lys HDAC8 substrate Co2+-D101E mutant 696240 3.5.1.98 0.16 - Fluor de Lys HDAC8 substrate Co2+-wild type 696240 3.5.1.98 0.16 - RHK(acetyl)K(acetyl)-fluorophore presence of Co(II), 25°C. pH 8.0 678192 3.5.1.98 0.21 - RHK(acetyl)K(acetyl)-fluorophore presence of Fe(II), 25°C. pH 8.0 678192 3.5.1.98 0.23 - Fluor de Lys HDAC8 substrate presence of activator N-(phenylcarbamothioyl)benzamide, pH not specified in the publication, temperature not specified in the publication 719061 3.5.1.98 0.744 - Fluor de Lys HDAC8 substrate pH not specified in the publication, temperature not specified in the publication 719061 3.5.1.98 1 - Fluor de Lys HDAC8 substrate Co2+-D101A mutant, larger than 1.00 696240 3.5.1.98 1 - Fluor de Lys HDAC8 substrate Co2+-D101L mutant, larger than 1.00 696240 3.5.1.98 1 - Fluor de Lys HDAC8 substrate Co2+-D101N mutant, larger than 1.00 696240 3.5.1.98 1 - Fluor de Lys HDAC8 substrate Co2+-H143A mutant, larger than 1.00 696240 3.5.1.98 1.1 - Fluor de Lys HDAC8 substrate Zn2+-wild type 696240 3.5.1.98 1.1 - RHK(acetyl)K(acetyl)-fluorophore presence of Zn(II), 25°C. pH 8.0 678192