3.4.21.7 additional information - additional information - 95553 3.4.21.7 additional information - additional information although neither plasmin nor urokinase-type plasminogen activator exhibits allosteric cooperativity, modeling shows that cooperativity occurs at the system level because of substrate competition, molecular modeling and simulation, substrate competition and bistability, overview 717386 3.4.21.7 additional information - additional information plasmin shows substrate binding site cooperativity, 717259 3.4.21.7 additional information - additional information ratio of kcat/Km values is 0.063 microM/s, pH 8.0, 37°C 695548 3.4.21.7 additional information - additional information the enzyme activity follows Michaelis-Menten kinetics 717666 3.4.21.7 0.00076 - hemofiltrate CC chemokine 1 - 652679 3.4.21.7 0.002 - GIYRSR pH 8.0 650822 3.4.21.7 0.003 - AIYRSR pH 8.0 650822 3.4.21.7 0.004 - CIYRSR pH 8.0 650822 3.4.21.7 0.0047 - GPGRVV pH 8.0 650822 3.4.21.7 0.005 - thrombin-activatable fibrinolysis inhibitor - 699871 3.4.21.7 0.0052 - 4-nitrophenyl-p'-(guanidinium)benzoate bromide plasmin 1 95551 3.4.21.7 0.0058 - GIYRSR pH 8.0 650822 3.4.21.7 0.00589 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the absence of fatty acids 686689 3.4.21.7 0.00817 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM stearate 686689 3.4.21.7 0.0113 - 4-nitrophenyl-p'-(guanidinium)benzoate bromide plasmin 2 95551 3.4.21.7 0.01133 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.115 mM stearate 686689 3.4.21.7 0.012 - GIVRSR pH 8.0 650822 3.4.21.7 0.01258 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.01 mM oleate 686689 3.4.21.7 0.0135 - benzyloxycarbonyl-Lys-p-nitrophenyl ester Glu1-plasminogen 95547 3.4.21.7 0.015 - benzyloxycarbonyl-Lys-p-nitrophenyl ester Lys77-plasminogen 95547 3.4.21.7 0.02 - D-Ile-Phe-Lys pH and temperature not specified in the publication 717919 3.4.21.7 0.02009 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.025 mM oleate 686689 3.4.21.7 0.021 - p-tosyl-Arg methyl ester - 95552 3.4.21.7 0.02337 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.175 mM stearate 686689 3.4.21.7 0.02371 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.01 mM arachidonate 686689 3.4.21.7 0.027 - p-nitrophenyl-p'-(thiouroniummethyl)benzoate plasmin 1 95551 3.4.21.7 0.02749 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.045 mM oleate 686689 3.4.21.7 0.03 - fibrinogen - 95552 3.4.21.7 0.036 - SPGRVV pH 8.0 650822 3.4.21.7 0.04 0.07 Spectrozyme PL at pH 7.4 and 37°C 754794 3.4.21.7 0.04265 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.025 mM arachidonate 686689 3.4.21.7 0.043 - p-nitrophenyl-p'-(thiouroniummethyl)benzoate plasmin 1 95551 3.4.21.7 0.05751 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.045 mM arachidonate 686689 3.4.21.7 0.05985 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM arachidonate 686689 3.4.21.7 0.07296 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.23 mM stearate 686689 3.4.21.7 0.083 - benzyloxycarbonyl-Lys-p-nitrophenyl ester Val561-plasminogen 95547 3.4.21.7 0.1 - LGGSGIYRSRSLE pH 8.0 650822 3.4.21.7 0.1311 - D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM oleate 686689 3.4.21.7 0.138 - D-Val-L-Leu-L-Lys-4-nitroanilide deletion mutant lacking the middle portion of the protein 701279 3.4.21.7 0.193 - D-Val-L-Leu-L-Lys-4-nitroanilide native enzyme 701279 3.4.21.7 0.21 - D-Val-Leu-Lys-p-nitroanilide Lys77-plasmin 95547 3.4.21.7 0.21 - LGGSGYKIGGSLE pH 8.0 650822 3.4.21.7 0.21 - p-nitrophenyl-p'-(pyridiniummethyl)-benzoate plasmin 1 95551 3.4.21.7 0.214 - p-nitrophenyl-p'-(methylethylsulfoniummethyl)benzoate plasmin 1 95551 3.4.21.7 0.23 - D-Val-Leu-Lys-p-nitroanilide plasmin adsorbed onto a carbon paste electrode 95564 3.4.21.7 0.23 - p-nitrophenyl-p'-(pyridiniummethyl)-benzoate plasmin 2 95551 3.4.21.7 0.258 - p-nitrophenyl-p'-(methylethylsulfoniummethyl)benzoate plasmin 2 95551 3.4.21.7 0.269 - D-Val-L-Leu-L-Lys-4-nitroanilide full-length plasmin, in phosphate buffered saline, pH 7.4, at 25°C 731263 3.4.21.7 0.28 - D-Val-Leu-Lys-p-nitroanilide Val442-plasmin 95547 3.4.21.7 0.325 - D-Val-L-Leu-L-Lys-4-nitroanilide truncated delta-plasmin, in phosphate buffered saline, pH 7.4, at 25°C 731263 3.4.21.7 0.33 - L-Ile-Phe-Lys pH and temperature not specified in the publication 717919 3.4.21.7 0.42 - LGGSGAVYKAGLE pH 8.0 650822 3.4.21.7 0.47 - LGGSGIYRVRSLE pH 8.0 650822 3.4.21.7 0.75 - LGGSIRYKGKSLE pH 8.0 650822 3.4.21.7 1.26 - D-Val-Leu-Lys-p-nitroanilide Val561-plasmin 95547 3.4.21.7 1.6 - LGGSAMSRMSSLE pH 8.0 650822 3.4.21.7 1.7 - LGGSGPYRSRSLE pH 8.0 650822 3.4.21.7 1.85 - LGGSGIGRSRSLE pH 8.0 650822 3.4.21.7 2.4 - LGGSGANFRGKLE pH 8.0 650822 3.4.21.7 2.9 - LGGSGTQRRLRLE pH 8.0 650822 3.4.21.7 3.7 - LGGSGIYRSVSLE pH 8.0 650822 3.4.21.7 5.1 - KKSPGRVVGGSVAH pH 8.0 650822 3.4.21.7 5.3 - tosyl-Arg methyl ester - 95551 3.4.21.7 5.3 - tosyl-Arg methyl ester enzyme form F-1 95550 3.4.21.7 6.8 - LGGSSIYRSRSLE pH 8.0 650822 3.4.21.7 7 - tosyl-Arg methyl ester - 95551 3.4.21.7 7 - tosyl-Arg methyl ester enzyme form F-2 95550