3.4.21.59 additional information - additional information - 29394 3.4.21.59 additional information - additional information effect of NaCl on Km 29383 3.4.21.59 additional information - additional information kinetics 683375 3.4.21.59 additional information - additional information kinetics and binding constants 683166 3.4.21.59 additional information - additional information overview: kinetic constant for the hydrolysis of synthetic thioester substrates and 4-nitroanilide substrates by the lung and by the skin enzyme 29375 3.4.21.59 additional information - additional information overview: Km values of peptide 4-nitroanilide substrates 29385, 29386 3.4.21.59 0.0002 - Benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide - 29393 3.4.21.59 0.0023 - bovine prothrombin - 29381 3.4.21.59 0.0046 - Tert-Butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide - 29393 3.4.21.59 0.0089 - Ac-PRNK-AAC pH 7.5, 30°C, betaII tryptase 652169 3.4.21.59 0.0138 - Benzyloxycarbonyl-L-Ala-L-Lys-L-Arg 4-methylcoumarin 7-amide - 29377 3.4.21.59 0.0145 - Ac-PRNK-AAC pH 7.5, 30°C, betaI tryptase 652169 3.4.21.59 0.0147 - Ac-PRTK-AAC pH 7.5, 30°C, betaII tryptase 652169 3.4.21.59 0.0148 - Benzyloxycarbonyl-L-Ala-L-Lys-L-Lys 4-methylcoumarin 7-amide - 29377 3.4.21.59 0.0165 - Ac-PRNR-AAC pH 7.5, 30°C, betaII tryptase 652169 3.4.21.59 0.0186 - Ac-PRNR-AAC pH 7.5, 30°C, betaI tryptase 652169 3.4.21.59 0.0231 - benzyloxycarbonyl-Arg 4-methylcoumarin 7-amide - 29393 3.4.21.59 0.0234 - Ac-PRTK-AAC pH 7.5, 30°C, betaI tryptase 652169 3.4.21.59 0.029 - tert-butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide - 29393 3.4.21.59 0.031 - tert-butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide - 29393 3.4.21.59 0.04 - Ala-Ala-Pro-Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.04 - Z-D-Arg-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 0.0409 - Benzyloxycarbonyl-L-Ala-L-Arg-L-Arg 4-methylcoumarin 7-amide - 29377 3.4.21.59 0.043 - t-butyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumarin 7-amide pH 8.0, 30°C 650440 3.4.21.59 0.044 - Pro-Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.049 - N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, tryptase beta/II 651932 3.4.21.59 0.05 - Ac-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.053 - Ala-Pro-Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.062 - Ac-Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.062 - D-Ile-Pro-Arg-4-nitroanilide pH 8.0, 25°C, active enzyme tetramer, in presence of heparin and Na+ 683166 3.4.21.59 0.071 - Ac-Pro-Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.081 - D-Ile-Pro-Arg-4-nitroanilide pH 6.8, 25°C, active enzyme tetramer, in presence of heparin and Na+ 683166 3.4.21.59 0.1 - Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.1105 - Ac-PANK-AAC pH 7.5, 30°C, betaII tryptase 652169 3.4.21.59 0.111 - Ile-Arg-Asn-Lys-5-amino-2-nitrobenzoylamide - 683808 3.4.21.59 0.12 - N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, D215G mutant 651932 3.4.21.59 0.1333 - Ac-PANK-AAC pH 7.5, 30°C, betaI tryptase 652169 3.4.21.59 0.15 - Z-D-Arg-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform S2 651154 3.4.21.59 0.19 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 1200 ng polybrene 650078 3.4.21.59 0.2 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 600 ng polybrene 650078 3.4.21.59 0.21 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 800 ng or 1000 ng polybrene 650078 3.4.21.59 0.23 - Z-D-Arg-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 0.27 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C 650078 3.4.21.59 0.3 - Bz-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 0.32 - S-2288 mutant enzyme Y75C, at pH 6.8 and 25°C 754178 3.4.21.59 0.323 - benzyloxycarbonyl-L-Ala-L-Leu-L-Lys-7-amido-4-methylcoumarin - 29377 3.4.21.59 0.35 - tosyl-Gly-Pro-Lys-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 0.36 - Z-D-Arg-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 0.37 - Glu-Pro-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 0.4 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 60 ng polybrene 650078 3.4.21.59 0.41 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 0.001 mg protamine 650078 3.4.21.59 0.42 - Glu-Pro-Arg-p-nitroanilide pH 7.6, 20°C, isoforms S1 and S2 651154 3.4.21.59 0.43 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 80 ng polybrene 650078 3.4.21.59 0.44 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 100 ng polybrene 650078 3.4.21.59 0.44 - tosyl-Gly-Pro-Lys-p-nitroanilide pH 7.6, 20°C, isoforms S1 and S2 651154 3.4.21.59 0.46 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C 650078 3.4.21.59 0.47 - S-2288 wild type enzyme, at pH 6.8 and 25°C 754178 3.4.21.59 0.49 - tosyl-Gly-Pro-Lys-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 0.53 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 0.0015 mg protamine 650078 3.4.21.59 0.58 - MeOCO-Nle-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 0.64 - Glu-Pro-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 0.64 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 6.0, 25°C, 0.002 mg protamine 650078 3.4.21.59 0.7 - D-Phe-Pip-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 0.78 - D-Phe-Pip-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 and S2 651154 3.4.21.59 0.79 - D-Phe-Pip-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 0.82 - S-2288 mutant enzyme I99C, at pH 6.8 and 25°C 754178 3.4.21.59 0.83 - MeOCO-Nle-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 0.922 - N-(p-tosyl)-Gly-Pro-Arg-4-nitroanilide pH 8.0, 22°C 683375 3.4.21.59 1 - S-2288 mutant enzyme I99C/Y75A/Y37bA, at pH 6.8 and 25°C 754178 3.4.21.59 1.04 - MeOCO-Nle-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 1.12 - D-Val-Leu-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 1.16 - MeOCO-Nle-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform S2 651154 3.4.21.59 1.19 - Bz-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 1.41 - D-Val-Leu-Arg-p-nitroanilide pH 7.6, 20°C, isoform S2 651154 3.4.21.59 1.6 - S-2288 mutant enzyme I99C*/Y75A/Y37bA, at pH 6.8 and 25°C 754178 3.4.21.59 1.64 - D-Pro-Phe-Arg-p-nitroanilide pH 7.6, 20°C, isoform L2 651154 3.4.21.59 1.7 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 100 ng protamine 650078 3.4.21.59 1.75 - D-Pro-Phe-Arg-p-nitroanilide pH 7.6, 20°C, isoform S2 651154 3.4.21.59 2.36 - Bz-Arg-p-nitroanilide pH 7.6, 20°C, isoform S2 651154 3.4.21.59 2.59 - D-Pro-Phe-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 2.849 - N-(p-tosyl)-Gly-Pro-Lys-p-nitroanilide pH 8.0, 22°C 683375 3.4.21.59 3.11 - D-Val-Leu-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 3.49 - D-Val-Leu-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 3.5 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, recombinant tryptase 653718 3.4.21.59 4.2 - N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, HMC-1 tryptase 653718 3.4.21.59 4.8 5 Bz-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 5.03 - Glu-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154 3.4.21.59 6.7 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 800 ng protamine 650078 3.4.21.59 7.4 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 400 ng protamine 650078 3.4.21.59 8.2 - D-Ile-Pro-Arg-p-nitroanilide pH 6.0, 25°C, 200 ng protamine 650078 3.4.21.59 10.5 - Glu-Gly-Arg-p-nitroanilide pH 7.6, 20°C, isoform S1 651154 3.4.21.59 13.5 - D-Pro-Phe-Arg-p-nitroanilide pH 7.6, 20°C, isoform L1 651154