2.7.4.9 additional information - additional information - 645194 2.7.4.9 0.00107 - dGMP pH 7.4, temperature not specified in the publication, NADH coupled assay 758798 2.7.4.9 0.0038 - 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, mutant enzyme F105Y 758582 2.7.4.9 0.0042 - TMP pH and temperature not specified in the publication, mutant enzyme F105Y 758582 2.7.4.9 0.0044 - D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate pH 7.6, 37°C 695005 2.7.4.9 0.0045 - 3'-azido-3'-deoxythymidine monophosphate pH 7.4, temperature not specified in the publication, NADH coupled assay 758798 2.7.4.9 0.0045 - dTMP - 691813 2.7.4.9 0.0045 - dTMP in 50 mM Tris-HCl pH 7.4, 50 mM KCl, 2 mM MgCl2, temperature not specified in the publication 722186 2.7.4.9 0.0045 - dTMP pH 7.4 662597 2.7.4.9 0.0045 - dTMP pH 7.4, 30°C 645207 2.7.4.9 0.0049 - dTMP pH 7.4, 37°C 645189 2.7.4.9 0.005 - dTMP pH 7.4 662597 2.7.4.9 0.005 - dTMP pH 7.4, 30°C 661477 2.7.4.9 0.005 - TMP pH 7.5, 25°C 645200 2.7.4.9 0.0051 - ATP pH 7.6, temperature not specified in the publication 759870 2.7.4.9 0.006 - ATP pH 7.5, 25°C, reaction with dTMP 645200 2.7.4.9 0.0062 - TMP pH 7.4, temperature not specified in the publication, NADH coupled assay 758798 2.7.4.9 0.0063 - TMP pH and temperature not specified in the publication, wild-type enzyme 758582 2.7.4.9 0.008 - 3'-fluoro-3'-deoxythymidine 5'-monophosphate pH 7.5, 25°C 645200 2.7.4.9 0.01 - 5-bromo-dUMP at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.012 - 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate pH 7.5, 25°C 645200 2.7.4.9 0.012 - 3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.5, 25°C 645200 2.7.4.9 0.012 - 3'-azido-3'-deoxythymidine monophosphate pH 7.4 662597 2.7.4.9 0.012 - 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, wild-type enzyme 758582 2.7.4.9 0.015 - TMP pH 7.4, 30°C 645201 2.7.4.9 0.015 - TMP pH 7.4, 30°C, wild-type enzyme 645202 2.7.4.9 0.017 - dTMP pH 7.4, temperature not specified in the publication 737398, 738163 2.7.4.9 0.02 - D-dTMP - 676227 2.7.4.9 0.02 - dTMP at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.02 - N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.022 - dTMP pH 7.2, 30°C 692915 2.7.4.9 0.022 - TMP - 705992 2.7.4.9 0.024 - N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-thymine at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.0242 - TMP mutant enzyme S108T, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.0255 - TMP wild-type enzyme, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.0258 - TMP mutant enzyme S108A, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.027 - ATP pH 7.5, 25°C, reaction with 3'-fluoro-3'-deoxythymidine 5'-monophosphate 645200 2.7.4.9 0.03 - dGMP - 705992 2.7.4.9 0.03 - dGMP pH 7.2, 30°C 692915 2.7.4.9 0.033 - ATP pH 7.5, 25°C, reaction with 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate 645200 2.7.4.9 0.033 - dTMP - 695005 2.7.4.9 0.0362 - dGMP wild-type enzyme, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.04 - ATP pH 7.4, 30°C, independent of cosubstrate 645202 2.7.4.9 0.04 - TMP pH 7.5 662169 2.7.4.9 0.045 - TMP pH 7.4, 30°C 645201 2.7.4.9 0.0476 - dGMP mutant enzyme S108A, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.0659 - TMP mutant enzyme Y153F, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.066 - ATP pH 7.4, temperature not specified in the publication 737398 2.7.4.9 0.066 - dTMP pH 8.5, 25°C 645203 2.7.4.9 0.069 - ATP pH 7.5, 25°C, reaction with 3'-azido-3'-deoxythymidine 5'-monophosphate 645200 2.7.4.9 0.0784 - dGMP mutant enzyme S108T, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.08 - 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.08 - 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C, wild-type enzyme 645202 2.7.4.9 0.09 - 3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.092 - ATP pH 7.5, temperature not specified in the publication 738558 2.7.4.9 0.0969 - dGMP mutant enzyme Y153F, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.1 - 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.1 - ATP pH 7.4, 30°C 645207 2.7.4.9 0.1 - ATP pH 7.5 662169 2.7.4.9 0.1 - N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-thymine at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.1008 - ATP wild-type enzyme, pH 7.4, temperature not specified in the publication 758833 2.7.4.9 0.11 - dTMP pH 7.5, temperature not specified in the publication 738558 2.7.4.9 0.13 - dATP pH 7.2, 37°C 645193 2.7.4.9 0.13 - N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.15 - N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.16 - N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.167 - L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate pH 7.6, 37°C 695005 2.7.4.9 0.17 - 3'-azido-3'-deoxythymidine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.17 - dUMP at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 0.19 - (E)-5-(2-bromovinyl)-deoxyuridine monophosphate - 706485 2.7.4.9 0.19 - dTMP pH 7.4, 37°C 645188 2.7.4.9 0.22 - 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.235 - ATP pH 8.5, 25°C 645203 2.7.4.9 0.24 - dTMP pH 7.8, 37°C 645198 2.7.4.9 0.25 - ATP pH 7.2, 37°C 645193 2.7.4.9 0.25 - dTMP pH 7.4, 30°C, mutant enzyme G146A 645202 2.7.4.9 0.27 - 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate pH 7.4, 30°C 645201 2.7.4.9 0.28 - 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C, mutant enzyme G146A 645202 2.7.4.9 0.33 - GTP pH 7.2, 37°C 645193 2.7.4.9 0.38 - L-dTMP - 676227 2.7.4.9 0.45 - dGTP pH 7.2, 37°C 645193 2.7.4.9 0.577 - dIMP pH 7.2, 30°C 692915 2.7.4.9 0.62 - ATP ATP in form of MgATP2- 645189 2.7.4.9 0.62 - ATP pH 7.4, 37°C 645189 2.7.4.9 0.78 - dUMP pH 7.2, 30°C 692915 2.7.4.9 1 - N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 1 - N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 1.1 - MgdATP2- - 645196 2.7.4.9 1.2 - ATP pH 7.8, 37°C 645198 2.7.4.9 1.5 - ATP ATP in form of MgATP2- 645196 2.7.4.9 1.5 - ATP pH 7.5, 37°C 645196 2.7.4.9 1.7 - N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-uracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 1.9 - N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 2.5 - dUMP pH 7.4, 30°C 645201 2.7.4.9 2.5 - dUMP pH 7.4, 30°C, wild-type enzyme 645202 2.7.4.9 2.5 - N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-uracil at 37°C in 50 mM Tris-HCl, pH 7.4, containing 50 mM KCl and 5 mM MgCl2 722915 2.7.4.9 3.8 - dUMP pH 7.4, 30°C 645201 2.7.4.9 4 - dUMP pH 7.4, 30°C, mutant enzyme G146A 645202