2.7.2.1 additional information - additional information assay methods for both directions of reaction 659856 2.7.2.1 additional information - additional information lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods 691204 2.7.2.1 additional information - additional information Michaelis-Menten kinetics 738717 2.7.2.1 additional information - additional information Michaelis-Menten kinetics, kinetic analysis and mechanism, detailed overview 739218 2.7.2.1 additional information - additional information Michaelis-Menten kinetics, thermodynamics 739591 2.7.2.1 additional information - additional information the dependence of the activity on acetate and acetyl phosphate concentrations obeys the Michaelis-Menten kinetics, whereas the dependence on ATP and ADP concentrations is sigmoidal 737465 2.7.2.1 additional information - additional information the turnover number of AckA1 is about an order of magnitude higher than that of AckA2 for the reaction in either direction. The Km values for acetyl phosphate, ATP, and ADP are similar for both isozymes. AckA2 has a higher affinity for acetate than does AckA1. Michaelis-Menten kinetics 738528 2.7.2.1 0.0026 - acetyl phosphate carried out at various temperatures 642183 2.7.2.1 0.016 - ATP mutant R91A, pH 7.0 659446 2.7.2.1 0.0222 - acetyl phosphate at pH 8.0 and 37°C 757471 2.7.2.1 0.0499 - ATP mutant V93A, pH 7.0 659065 2.7.2.1 0.0506 - ATP mutant F179A, pH 7.0 659065 2.7.2.1 0.053 - ATP Q43W, mutant 690888 2.7.2.1 0.0542 - ATP mutant P232A, pH 7.0 659065 2.7.2.1 0.0576 - ATP mutant L122A, pH 7.0 659065 2.7.2.1 0.063 - ADP mutant R91A, pH 7.4 659446 2.7.2.1 0.063 - ADP mutant R91L, pH 7.4 659446 2.7.2.1 0.068 - ATP mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 0.068 - ATP wild-type, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 0.0687 - ATP mutant V93G, pH 7.0 659065 2.7.2.1 0.07 - ATP - 642182 2.7.2.1 0.07 - ATP co-substrate: acetate, pH 7.5, 37°C 721949 2.7.2.1 0.07 - ATP pH 7.5, 30°C, recombinant His-tagged isozyme AckA1 738528 2.7.2.1 0.07 - ATP pH 7.5, 30°C, recombinant His-tagged isozyme AckA2 738528 2.7.2.1 0.07 - ATP pH 7.9, 37°C 642182 2.7.2.1 0.0713 - ATP wild-type, pH 7.0 659065 2.7.2.1 0.075 - ATP co-substrate: propionate, pH 7.5, 37°C 721949 2.7.2.1 0.076 - ATP mutant R91K, pH 7.0 659446 2.7.2.1 0.078 - GTP co-substrate: acetate, pH 7.5, 37°C 721949 2.7.2.1 0.08 - ATP R241A/Q43W, double mutant 690888 2.7.2.1 0.08 - ATP wild-type 690888 2.7.2.1 0.08 - ATP wild-type, pH 7.0 659446 2.7.2.1 0.087 - ADP carried out at various temperatures 642183 2.7.2.1 0.088 - CTP co-substrate: acetate, pH 7.5, 37°C 721949 2.7.2.1 0.096 - UTP co-substrate: acetate, pH 7.5, 37°C 721949 2.7.2.1 0.098 - ADP wild-type 690888 2.7.2.1 0.098 - ADP wild-type, pH 7.4 659446 2.7.2.1 0.1 - acetyl phosphate pH 7.4, 25°C 642176 2.7.2.1 0.1 - ADP co-substrate: acetate, pH 7.5, 37°C 721949 2.7.2.1 0.11 - acetyl phosphate pH 8.0, 30°C 737465, 746769 2.7.2.1 0.14 - Propionyl phosphate pH 7.5, 30°C 642181 2.7.2.1 0.145 - ATP mutant R91L, pH 7.0 659446 2.7.2.1 0.147 - ADP R91A/Q43W, double mutant 690888 2.7.2.1 0.15 - acetyl phosphate mutant F179A, pH 7.4 659065 2.7.2.1 0.16 - acetyl phosphate - 642182 2.7.2.1 0.16 - acetyl phosphate pH 7.9, 37°C 642182 2.7.2.1 0.164 - ADP R91K/Q43W, double mutant 690888 2.7.2.1 0.168 - ADP mutant R91K, pH 7.4 659446 2.7.2.1 0.173 - ADP Q43W, mutant 690888 2.7.2.1 0.188 - ADP at pH 8.0 and 37°C 757471 2.7.2.1 0.2 - acetyl phosphate pH 7.4, 25°C, 0.5 M NaCl 642176 2.7.2.1 0.2 - ADP pH 7.5, 30°C 642181 2.7.2.1 0.21 - ADP mutant R241A, pH 7.4 659446 2.7.2.1 0.22 - ATP pH 7.5, 30°C 642181 2.7.2.1 0.234 - ATP R91K/Q43W, double mutant 690888 2.7.2.1 0.239 - ADP R241A/Q43W, double mutant 690888 2.7.2.1 0.24 - ADP pH 7.4, 25°C 642176 2.7.2.1 0.26 - acetyl phosphate Q43W 690888 2.7.2.1 0.264 - ATP mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 0.28 - acetyl phosphate pH 7.5, 37°C 721949 2.7.2.1 0.297 - ATP mutant R241A, pH 7.0 659446 2.7.2.1 0.3 - ADP pH 7.4, 37°C 642173 2.7.2.1 0.3 - ADP pH 7.5, 30°C 642195 2.7.2.1 0.3 - ATP pH 7.4, 25°C 642176 2.7.2.1 0.33 - acetyl phosphate R241/Q43W 690888 2.7.2.1 0.34 - acetyl phosphate wild-type, pH 7.4 659065 2.7.2.1 0.37 - ATP pH 7.3, 30°C 642189 2.7.2.1 0.44 - acetyl phosphate pH 7.0, 50°C 487537 2.7.2.1 0.44 - acetyl phosphate pH 7.5, 30°C 642181 2.7.2.1 0.47 - acetyl phosphate wild-type 690888 2.7.2.1 0.47 - acetyl phosphate wild-type, pH 7.4 659446 2.7.2.1 0.47 - ADP pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.47 - ADP pH 7.5, 30°C, recombinant His-tagged isozyme AckA1 738528 2.7.2.1 0.48 - ATP pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.48 - ATP pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.49 - ADP pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.49 - ATP pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.5 - acetyl phosphate mutant L122A, pH 7.4 659065 2.7.2.1 0.5 - acetyl phosphate pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.5 - ADP pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.5 - ADP pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.5 - ADP pH 7.9, 37°C 642182 2.7.2.1 0.5 - ATP acetate kinase EutP, at 40°C, pH not specified in the publication 761931 2.7.2.1 0.5 - ATP acetate kinase EutQ, at 40°C, pH not specified in the publication 761931 2.7.2.1 0.5 - ATP pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.53 - acetyl phosphate pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.53 - acetyl phosphate pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.53 - acetyl phosphate pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.539 - ATP R91A/Q43W, double mutant 690888 2.7.2.1 0.54 - acetyl phosphate pH 7.5, 30°C, recombinant His-tagged isozyme AckA1 738528 2.7.2.1 0.55 - acetyl phosphate pH 7.5, 30°C, recombinant His-tagged isozyme AckA2 738528 2.7.2.1 0.57 - ATP pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.578 - ADP mutant R241K, pH 7.4 659446 2.7.2.1 0.58 - acetyl phosphate pH 7.3, 30°C 642189 2.7.2.1 0.59 - acetyl phosphate pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.59 - ATP pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.6 - ATP pH 7.5, 30°C 642195 2.7.2.1 0.61 - acetyl phosphate mutant R91K, pH 7.4 659446 2.7.2.1 0.61 - ADP pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 0.62 - ADP pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.64 - ADP pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.64 - ATP pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.65 - ATP pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.66 - ADP pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.66 - ATP pH 6.5, 30°C, recombinant mutant N213T/G332D/E336L/T385N 738717 2.7.2.1 0.67 - acetyl phosphate pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 0.67 - ATP pH 6.5, 30°C, recombinant wild-type enzyme 738717 2.7.2.1 0.69 - acetyl phosphate pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 0.692 - ADP R241K/Q43W 690888 2.7.2.1 0.7 - acetate acetate kinase EutP, at 40°C, pH not specified in the publication 761931 2.7.2.1 0.7 - acetate acetate kinase EutQ, at 40°C, pH not specified in the publication 761931 2.7.2.1 0.7 - ATP pH 7.0, 20°C, mutant E334A, produced in E. coli 642191 2.7.2.1 0.7 - ATP pH 7.0, 37°C, mutant H180A, produced in E. coli 642193 2.7.2.1 0.7 - ATP pH 7.0, 50°C 487537 2.7.2.1 0.71 - acetyl phosphate mutant V93G, pH 7.4 659065 2.7.2.1 0.71 - acetyl phosphate pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 0.71 - ADP pH 7.3, 30°C 642189 2.7.2.1 0.74 - acetyl phosphate mutant V93A, pH 7.4 659065 2.7.2.1 0.74 - ADP pH 7.5, 30°C, recombinant His-tagged isozyme AckA2 738528 2.7.2.1 0.78 - ITP pH 7.9, 37°C 642182 2.7.2.1 0.84 - acetyl phosphate mutant R241A, pH 7.4 659446 2.7.2.1 0.9 - ATP pH 7.6, 37°C, mutant R340L 642194 2.7.2.1 0.92 - acetyl phosphate mutant R241K, pH 7.4 659446 2.7.2.1 0.94 - ATP pH 7.9, 37°C 642182 2.7.2.1 1 - acetyl phosphate pH 7.5, 30°C 642195 2.7.2.1 1 - ATP pH 7.0, 20°C, unaltered enzyme, produced in E. coli 642191 2.7.2.1 1 - ATP pH 7.6, 37°C, wild-type and mutant R175K 642194 2.7.2.1 1 2 acetate pH 7.0, temperature not specified in the publication 727558 2.7.2.1 1.02 - acetyl phosphate R241K/Q43W 690888 2.7.2.1 1.08 - acetyl phosphate R91K/Q43W 690888 2.7.2.1 1.1 - ADP pH 7.3, 37°C, 1.0 M succinate 642171 2.7.2.1 1.1 - ATP carried out at various temperatures 642183 2.7.2.1 1.1 - ATP pH 7.0, 20°C, mutant E32A, produced in E. coli 642191 2.7.2.1 1.1 - GTP pH 7.9, 37°C 642182 2.7.2.1 1.12 - ADP pH 7.5, 25°C 642197 2.7.2.1 1.19 - acetyl phosphate mutant P232A, pH 7.4 659065 2.7.2.1 1.2 - acetate pH 7.5, 37°C 721949 2.7.2.1 1.2 - acetyl phosphate pH 7.4, 37°C 642173 2.7.2.1 1.2 - acetyl phosphate wild type enzyme, at pH 7.0 and 37°C 762441 2.7.2.1 1.26 - ADP mutant R241L, pH 7.4 659446 2.7.2.1 1.28 - ADP pH 8.0, 30°C 737465 2.7.2.1 1.3 - ADP pH 7.3, 37°C 642175 2.7.2.1 1.3 - ADP pH 7.3, 37°C, 0.2 M succinate 642171 2.7.2.1 1.3 - ATP pH 7.6, 37°C, mutant R340K 642194 2.7.2.1 1.3 - ATP R241K/Q43W, double mutant 690888 2.7.2.1 1.3 - UTP carried out at various temperatures 642183 2.7.2.1 1.36 - acetyl phosphate mutant R91A, pH 7.4 659446 2.7.2.1 1.4 - ATP - 642194 2.7.2.1 1.4 - ATP pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli 642191 2.7.2.1 1.4 - ATP pH 7.0, 37°C, mutant H180R, produced in E. coli 642193 2.7.2.1 1.5 - acetate wild-type, pH 7.0 659065 2.7.2.1 1.5 - ADP pH 7.4 642166 2.7.2.1 1.5 - ATP - 642196 2.7.2.1 1.5 - ATP pH 7.0, 37°C, mutant H123A, produced in E. coli 642193 2.7.2.1 1.53 - ADP pH 7.4, 25°C, 0.5 M NaCl 642176 2.7.2.1 1.6 - ATP pH 7.0, 20°C, mutant E385C, produced in E. coli 642191 2.7.2.1 1.61 - acetate pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 1.63 - acetate pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 1.64 - acetyl phosphate mutant R241L, pH 7.4 659446 2.7.2.1 1.7 - acetyl phosphate mutant enzyme G331Q/I332M, at pH 7.0 and 37°C 762441 2.7.2.1 1.7 - ATP 37°C, pH 7.0-7.5, mutant enzyme G239A 730304 2.7.2.1 1.7 - ATP pH 6.5, 30°C, recombinant mutant N213T 738717 2.7.2.1 1.7 - ATP pH 7.0, 37°C, mutant H90A, produced in E. coli 642193 2.7.2.1 1.7 - ATP wild type enzyme, at pH 5.5 and 45°C 762441 2.7.2.1 1.71 - acetate pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 1.77 - acetate pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 1.8 - ADP wild type enzyme, at pH 7.0 and 37°C 762441 2.7.2.1 1.8 - ATP 37°C, pH 7.0-7.5, mutant enzyme G239S 730304 2.7.2.1 1.9 - ATP pH 7.0, 37°C, mutant H208A, produced in E. coli 642193 2.7.2.1 1.9 - CTP carried out at various temperatures 642183 2.7.2.1 2 - ATP pH 7.0, 20°C, mutant E385Q, produced in E. coli 642191 2.7.2.1 2 - ATP pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli 642193 2.7.2.1 2 - ATP pH 7.4, 37°C 642173 2.7.2.1 2.13 - ATP pH 7.4, 37°C, wild-type 642196 2.7.2.1 2.3 - acetate - 642194 2.7.2.1 2.3 - acetate pH 7.0, 20°C, mutant E97A, produced in E. coli 642191 2.7.2.1 2.3 - acetyl phosphate mutant R91L, pH 7.4 659446 2.7.2.1 2.36 - ATP pH 8.0, 30°C 737465 2.7.2.1 2.5 - ATP pH 7.0, 36°C 642187 2.7.2.1 2.58 - acetyl phosphate R91A/Q43W 690888 2.7.2.1 2.7 - acetate wild-type 690888 2.7.2.1 2.7 - acetate wild-type, pH 7.0 659446 2.7.2.1 2.7 - ATP pH 7.0, 37°C, mutant H184A, produced in E. coli 642193 2.7.2.1 2.7 - TTP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 2.7 - UTP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 2.8 - acetate mutant E97D, pH 7.5, 37°C 659856 2.7.2.1 2.8 - ATP - 642191 2.7.2.1 2.8 - ATP 37°C, pH is not specified in the publication 727804 2.7.2.1 2.8 - ATP pH 7.0, 37°C, authentic wild-type enzyme 642193 2.7.2.1 2.8 - ATP pH 7.3, 37°C 642184 2.7.2.1 2.8 - ATP pH 7.6, 37°C, recombinant wild-type 642194 2.7.2.1 3 - ADP pH 7.0, 50°C 487537 2.7.2.1 3 - ATP pH 7.0, 37°C, mutant H60A, produced in E. coli 642193 2.7.2.1 3 - ATP pH 7.4, 37°C, mutant N211A 642196 2.7.2.1 3 - ATP pH 7.6, 37°C, mutant R285K, R285L 642194 2.7.2.1 3.1 - ATP pH 7.4, 37°C, mutant D148A 642196 2.7.2.1 3.1 - ATP with acetate as cosubstrate, at pH 5.7 and 37°C 760452 2.7.2.1 3.2 - ATP pH 7.4, 37°C, mutant S10A 642196 2.7.2.1 3.2 - CTP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 3.3 - acetyl phosphate pH 7.3, 37°C, 2.0 M succinate 642171 2.7.2.1 3.4 - ATP pH 7.6, 37°C, mutant R241K 642194 2.7.2.1 3.5 - ATP pH 6.5, 30°C, recombinant mutant G332D 738717 2.7.2.1 3.62 - ATP pH 7.4, 37°C, mutant K14R 642196 2.7.2.1 3.7 - ATP pH 7.0, 37°C, mutant H180Q, produced in E. coli 642193 2.7.2.1 3.7 - ATP pH 7.4, 37°C, mutant S12T 642196 2.7.2.1 3.7 - ATP pH 7.5, 30°C, isoenzyme II 642179 2.7.2.1 3.7 - ITP carried out at various temperatures 642183 2.7.2.1 3.8 - ATP pH 7.5, 30°C, isoenzyme I 642179 2.7.2.1 3.9 - ATP pH 7.6, 37°C, mutant R91K 642194 2.7.2.1 4 - acetate pH 7.0, 20°C, mutant E97Q, produced in E. coli 642191 2.7.2.1 4 - acetate pH 7.6, 37°C, mutant R175K 642194 2.7.2.1 4 - ATP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 4.3 - ATP pH 7.0, 20°C, mutant E385D, produced in E. coli 642191 2.7.2.1 4.3 - ATP pH 7.4, 37°C, mutant S10T 642196 2.7.2.1 4.5 - ATP - 642191 2.7.2.1 4.5 - ATP pH 7.4, 37°C, mutant S11A 642196 2.7.2.1 4.6 - ATP pH 7.0, 37°C, mutant H180N, produced in E. coli 642193 2.7.2.1 4.7 - ATP pH 7.0, 37°C, mutant H180D, produced in E. coli 642193 2.7.2.1 4.7 - ITP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 4.9 - acetate pH 7.5, 30°C, recombinant His-tagged isozyme AckA2 738528 2.7.2.1 5 - acetyl phosphate pH 7.3, 37°C, 0.2 M succinate 642171 2.7.2.1 5 - acetyl phosphate pH 7.4 642166 2.7.2.1 5 - ATP pH 7.4, 37°C, mutant N7D, D148E 642196 2.7.2.1 5.2 - ATP pH 6.5, 30°C, recombinant mutant G333Q 738717 2.7.2.1 5.2 - ATP pH 7.4, 37°C, mutant K14A 642196 2.7.2.1 5.3 - GTP 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 5.6 - acetate pH 8.0, 30°C 737465, 746769 2.7.2.1 5.7 - ADP mutant enzyme G331Q/I332M, at pH 7.0 and 37°C 762441 2.7.2.1 5.7 - GTP carried out at various temperatures 642183 2.7.2.1 5.8 - acetate R241A/Q43W, double mutant 690888 2.7.2.1 5.8 - ATP pH 7.0, 37°C, mutant H94A, produced in E. coli 642193 2.7.2.1 5.8 - ATP pH 7.4, 37°C, mutant S10T 642196 2.7.2.1 5.9 - ATP 37°C, pH 7.0-7.5, mutant enzyme N211A 730304 2.7.2.1 6 - ADP pH 7.0, 36°C 642187 2.7.2.1 6 - ATP pH 7.3, 37°C 642175 2.7.2.1 6 - ATP pH 7.6, 37°C, mutant R285A, R91A 642194 2.7.2.1 6.2 - ATP pH 7.0, 37°C, mutant H180E, produced in E. coli 642193 2.7.2.1 6.2 - propionate mutant V93A, pH 7.0 659065 2.7.2.1 6.3 - ATP pH 7.0, 37°C, mutant H180K, produced in E. coli 642193 2.7.2.1 6.6 - ATP pH 6.5, 30°C, recombinant mutant I334M 738717 2.7.2.1 6.8 - ATP 37°C, pH 7.0-7.5, mutant enzyme N211S 730304 2.7.2.1 6.8 - ATP pH 7.4, 37°C, mutant S11T 642196 2.7.2.1 6.9 - ATP pH 7.0, 20°C, mutant E385A, produced in E. coli 642191 2.7.2.1 7 - acetate pH 7.9, 37°C 642182 2.7.2.1 7 - acetyl phosphate pH 7.3, 37°C 642175 2.7.2.1 7 - ATP pH 7.4, 37°C, mutant N7A 642196 2.7.2.1 7.1 - ATP 37°C, pH 7.0-7.5, mutant enzyme N211T 730304 2.7.2.1 7.2 - acetate - 642194 2.7.2.1 7.2 - acetate pH 7.0, 20°C, mutant E97D, produced in E. coli 642191 2.7.2.1 7.2 - GTP 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 7.3 - ATP pH 7.4, 37°C, mutant S12A 642196 2.7.2.1 7.4 - GTP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 7.7 - ATP pH 7.4, 37°C, mutant E384A 642196 2.7.2.1 9 - ATP pH 7.3, 37°C, 50 M succinate 642171 2.7.2.1 9.4 - ATP mutant enzyme G331Q/I332M, at pH 5.5 and 45°C 762441 2.7.2.1 10 - acetate pH 7.0, 37°C, mutant H180R, produced in E. coli 642193 2.7.2.1 10 - ATP pH 7.3, 37°C, 5.0 M succinate 642171 2.7.2.1 10.1 - ITP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 10.2 - ATP 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 10.4 - acetate mutant F179A, pH 7.0 659065 2.7.2.1 10.7 - ITP 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 10.7 - propionate mutant L122A, pH 7.0 659065 2.7.2.1 11 - propionate mutant F179A, pH 7.0 659065 2.7.2.1 11.1 - CTP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 11.2 - propionate pH 7.5, 37°C 721949 2.7.2.1 11.2 - TTP 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 11.4 - ATP mutant R241K, pH 7.0 659446 2.7.2.1 11.7 - acetate pH 7.5, 30°C, isoenzyme I 642179 2.7.2.1 12.1 - TTP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 12.4 - acetate Q43W, mutant 690888 2.7.2.1 13 - acetate pH 7.0, 37°C, mutant H180D, produced in E. coli 642193 2.7.2.1 13.5 - ATP co-substrate: formate, pH 7.5, 37°C 721949 2.7.2.1 13.5 - formate pH 7.5, 37°C 721949 2.7.2.1 14 - acetate pH 7.0, 37°C, mutant H180Q, produced in E. coli 642193 2.7.2.1 14.2 - UTP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 14.2 - valerate at pH 5.7 and 37°C 760452 2.7.2.1 14.3 - acetate 37°C, pH 7.0-7.5, wild-type enzyme 730304 2.7.2.1 14.4 - propionate wild-type, pH 7.0 659065 2.7.2.1 15 - acetate pH 7.0, 20°C, mutant E334A, produced in E. coli 642191 2.7.2.1 15 - acetate pH 7.0, 37°C, mutant H184A, produced in E. coli 642193 2.7.2.1 15.7 - CTP 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 16 - acetate pH 7.0, 37°C, mutant H123A, produced in E. coli 642193 2.7.2.1 17 - acetate pH 7.0, 20°C, mutant E385C, produced in E. coli 642191 2.7.2.1 17 - acetate pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli 642193 2.7.2.1 17 - ATP mutant R241L, pH 7.0 659446 2.7.2.1 18 - acetate pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli 642193 2.7.2.1 18.4 - ATP 37°C, pH 7.0-7.5, mutant enzyme G331Q 730304 2.7.2.1 19 - acetate pH 7.0, 20°C, unaltered enzyme, produced in E. coli 642191 2.7.2.1 19 - acetate pH 7.0, 37°C, mutant H180K, produced in E. coli 642193 2.7.2.1 19 - acetate pH 7.6, 37°C 642194 2.7.2.1 19.5 - acetate mutant L122A, pH 7.0 659065 2.7.2.1 20 - acetate pH 7.0, 37°C, mutant H60A, produced in E. coli 642193 2.7.2.1 20 - acetate pH 7.6, 37°C, mutant R340L 642194 2.7.2.1 20 - acetate wild type enzyme, at pH 5.5 and 45°C 762441 2.7.2.1 20.54 - acetate pH 7.5, 30°C, recombinant His-tagged isozyme AckA1 738528 2.7.2.1 21.4 - acetate mutant V93A, pH 7.0 659065 2.7.2.1 22 - acetate - 642191 2.7.2.1 22 - acetate 37°C, pH is not specified in the publication 727804 2.7.2.1 22 - acetate pH 7.0, 37°C, authentic wild-type enzyme 642193 2.7.2.1 22 - acetate pH 7.3, 37°C 642184 2.7.2.1 22 - acetate wild-type, pH 7.5, 37°C 659856 2.7.2.1 24 - acetate wild-type, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 24 - propionate wild-type, pH 7.5, 37°C 659856 2.7.2.1 24.5 - Butyrate at pH 5.7 and 37°C 760452 2.7.2.1 25 - propionate mutant V93G, pH 7.0 659065 2.7.2.1 25.8 - propionate at pH 5.7 and 37°C 760452 2.7.2.1 26 - acetate pH 7.0, 20°C, mutant E32A, produced in E. coli 642191 2.7.2.1 29.3 - acetate pH 7.5, 25°C, recombinant enzyme 739591 2.7.2.1 30 - acetate mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 30 - acetate pH 7.4, 37°C 642173 2.7.2.1 30.7 - ATP pH 6.5, 30°C, recombinant mutant N337E 738717 2.7.2.1 31.32 - acetate pH 7.5, 20°C, recombinant enzyme 739591 2.7.2.1 32 - acetate mutant P232A, pH 7.0 659065 2.7.2.1 32.26 - acetate pH 7.5, 15°C, recombinant enzyme 739591 2.7.2.1 33 - acetate pH 7.0, 37°C, mutant H208A, produced in E. coli 642193 2.7.2.1 33.4 - Butyrate mutant V93A, pH 7.0 659065 2.7.2.1 36.02 - acetate pH 7.5, 10°C, recombinant enzyme 739591 2.7.2.1 38.5 - acetate pH 7.4, 25°C 642176 2.7.2.1 38.5 - acetate pH 7.5, 30°C, isoenzyme II 642179 2.7.2.1 39 - Butyrate wild-type, pH 7.0 659065 2.7.2.1 39.5 - acetate mutant V93G, pH 7.0 659065 2.7.2.1 40 - acetate carried out at various temperatures 642183 2.7.2.1 40 - acetate pH 7.0, 50°C 487537 2.7.2.1 40 - propionate pH 7.5, 30°C, isoenzyme I 642179 2.7.2.1 42 - acetate pH 7.6, 37°C, mutant R340K 642194 2.7.2.1 44 - acetate pH 7.0, 20°C, mutant E385Q, produced in E. coli 642191 2.7.2.1 46 - propionate mutant P232A, pH 7.0 659065 2.7.2.1 48 - acetate pH 7.4, 37°C, wild-type 642196 2.7.2.1 48 - acetate pH 7.5, 30°C 642195 2.7.2.1 48 - acetate R91K/Q43W, double mutant 690888 2.7.2.1 53.9 - acetate at pH 5.7 and 37°C 760452 2.7.2.1 56 - ATP pH 7.0, 37°C, mutant H180A, produced in E. coli 642193 2.7.2.1 63 - Butyrate mutant V93G, pH 7.0 659065 2.7.2.1 63.3 - propionate pH 7.5, 30°C 642181 2.7.2.1 66 - acetate - 642196 2.7.2.1 66 - acetate pH 7.0, 37°C, mutant H94A, produced in E. coli 642193 2.7.2.1 70 - acetate mutant R91K, pH 7.0 659446 2.7.2.1 73 - acetate pH 7.3, 30°C 642189 2.7.2.1 77 - acetate mutant R241K, pH 7.0 659446 2.7.2.1 80.9 - acetate pH 7.5, 30°C 642181 2.7.2.1 83 - acetate mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl 659446 2.7.2.1 86 - acetate pH 7.0, 20°C, mutant E385A, produced in E. coli 642191 2.7.2.1 90 - acetate pH 7.4 642166 2.7.2.1 94 - acetate 37°C, pH 7.0-7.5, mutant enzyme G239S 730304 2.7.2.1 100 - acetate pH 7.3, 37°C, 50 M succinate 642171 2.7.2.1 109 - Isobutyrate at pH 5.7 and 37°C 760452 2.7.2.1 111 - acetate pH 7.4, 37°C, mutant N211A 642196 2.7.2.1 113 - acetate 37°C, pH 7.0-7.5, mutant enzyme G239A 730304 2.7.2.1 129 - acetate pH 7.6, 37°C, mutant R285L 642194 2.7.2.1 133 - propionate pH 7.5, 30°C, isoenzyme II 642179 2.7.2.1 135 - acetate - 642170 2.7.2.1 135 - acetate pH 7.2-8.0, 50°C 642190 2.7.2.1 140 - acetate 37°C, pH 7.0-7.5, mutant enzyme N211S 730304 2.7.2.1 150 - propionate carried out at various temperatures 642183 2.7.2.1 160 - acetate pH 7.0, 36°C 642187 2.7.2.1 165 - acetate 37°C, pH 7.0-7.5, mutant enzyme N211A 730304 2.7.2.1 170 - acetate pH 7.3, 37°C, 5.0 M succinate 642171 2.7.2.1 170 - acetate pH 7.4, 37°C, mutant D148A 642196 2.7.2.1 179 - acetate R241K/Q43W, double mutant 690888 2.7.2.1 200 - acetate pH 7.4, 37°C, mutant S11A 642196 2.7.2.1 200 - Butyrate carried out at various temperatures 642183 2.7.2.1 206 - acetate pH 7.6, 37°C, mutant R285A 642194 2.7.2.1 219 - acetate pH 7.6, 37°C, mutant R285K 642194 2.7.2.1 239 - acetate pH 7.4, 37°C, mutant K14R 642196 2.7.2.1 250 - acetate mutant R91A, pH 7.0 659446 2.7.2.1 260 - acetate pH 7.6, 37°C, mutant R91K 642194 2.7.2.1 266 - acetate pH 7.4, 37°C, mutant S10T 642196 2.7.2.1 270 - acetate mutant R241L, pH 7.0 659446 2.7.2.1 300 - acetate pH 7.4 642166 2.7.2.1 300 - propionate pH 7.3, 37°C, 50 M succinate 642171 2.7.2.1 302 - acetate mutant enzyme G331Q/I332M, at pH 5.5 and 45°C 762441 2.7.2.1 330 - acetate pH 7.0, 37°C, mutant H90A, produced in E. coli 642193 2.7.2.1 333 - acetate pH 7.4, 37°C, mutant S12T 642196 2.7.2.1 337 - acetate pH 7.0, 20°C, mutant E385D, produced in E. coli 642191 2.7.2.1 390 - acetate pH 7.4, 37°C, mutant D148N 642196 2.7.2.1 420 - acetate mutant R91L, pH 7.0 659446 2.7.2.1 420 - isobuytyrate carried out at various temperatures 642183 2.7.2.1 470 - acetate 37°C, pH 7.0-7.5, mutant enzyme N211T 730304 2.7.2.1 601 - acetate 37°C, pH 7.0-7.5, mutant enzyme G331A 730304 2.7.2.1 693 - acetate pH 7.4, 37°C, mutant N7D 642196 2.7.2.1 710 - acetate mutant R241A, pH 7.0 659446 2.7.2.1 800 - acetate pH 7.3, 37°C 642175 2.7.2.1 814 - acetate pH 7.4, 37°C, mutant S10A 642196 2.7.2.1 1000 - propionate pH 7.3, 37°C, 5.0 M succinate 642171 2.7.2.1 1573 - acetate pH 7.4, 37°C, mutant S11T 642196