2.6.1.85 0.0016 - chorismate apparent K0.5 of chorismate for the activation of glutamine amidotransferase (calculated from chorismate-dependent activation), pH 8.0, 37°C 724111 2.6.1.85 0.0022 - chorismate p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C 724111 2.6.1.85 0.009 - chorismate NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C 724111 2.6.1.85 0.013 - chorismate wild-type, cosubstrate L-glutamine, pH 8.5, 25°C 661933 2.6.1.85 0.02 - chorismate cosubstrate L-glutamine, pH 7.0, 25°C 724464 2.6.1.85 0.045 - L-glutamine glutamate production in the presence of chorismate, pH 8.0, 37°C 724111 2.6.1.85 0.05 - L-glutamine glutamate production in the absence of chorismate, pH 8.0, 37°C 724111 2.6.1.85 0.058 - chorismate wild-type, cosubstrate NH4+, pH 8.5, 25°C 661933 2.6.1.85 0.064 - L-glutamine p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C 724111 2.6.1.85 0.097 - chorismate mutant K274A, cosubstrate NH4+, pH 8.5, 25°C 661933 2.6.1.85 0.117 - chorismate pH 8.0, 25°C 738864 2.6.1.85 0.216 - chorismate cosubstrate L-glutamine, pH 7.0, 25°C 724464 2.6.1.85 0.24 - chorismate PabB, pH 8.0, 25°C 724331 2.6.1.85 2.4 - L-glutamine pH 8.0, 25°C 738864 2.6.1.85 11 - L-glutamine pH 7.0, 25°C 724464 2.6.1.85 145 - NH4+ wild-type, pH 8.5, 25°C 661933 2.6.1.85 169 - NH4+ mutant K274A, pH 8.5, 25°C 661933 2.6.1.85 195 - NH3 NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C 724111