2.1.1.228 0.00123 - inosine37 in yeast tRNAAsp possessing a G36I37 sequence below 0.00123 pH 8.0, 37°C 661154 2.1.1.228 additional information - additional information binding kinetics of TrmD ligands 757409 2.1.1.228 additional information - additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families 712739 2.1.1.228 additional information - additional information kinetics and thermodynamics analysis, overview. Steady-state kinetics with S-adenosyl-L-methionine (SAM) and tRNALeu(GAG) show that PaTrmD catalyzes the two-substrate reaction by way of a ternary-complex, while isothermal titration calorimetry revealed that SAM and tRNALeu(GAG) bind to PaTrmD independently, each with a dissociation constant of 0.014 mM 758329 2.1.1.228 additional information - additional information KM-values for truncated tRNAPhe variants 673850 2.1.1.228 additional information - additional information measurement of the pre-steady-state rate constant of methyl transfer of TrmD, a proton abstraction step is rate limiting, steady-state kinetics 735927 2.1.1.228 additional information - additional information Michaelis-Menten kinetic analysis 756161 2.1.1.228 additional information - additional information Michaelis-Menten steady-state kinetics analysis 736428 2.1.1.228 0.0008 - guanine37 in tRNALeu(GAG) pH 7.5, 37°C, recombinant enzyme 758329 2.1.1.228 0.003 - S-adenosyl-L-methionine pH 7.5, 37°C, recombinant enzyme 758329 2.1.1.228 0.0004 - guanine37 in Aquifex aeolicus tRNAGln(UUG) pH 7.6, 60°C 673850 2.1.1.228 0.0005 - guanine37 in Haloferax volcanii tRNALeu(CAA) pH 7.6, 60°C 673850 2.1.1.228 0.0006 - guanine37 in Aquifex aeolicus tRNAArg(ACG) pH 7.6, 60°C 673850 2.1.1.228 0.0006 - guanine37 in Aquifex aeolicus tRNAHis(GUG) pH 7.6, 60°C 673850 2.1.1.228 0.0007 - guanine37 in Aquifex aeolicus tRNAPro(GGG) pH 7.6, 60°C 673850 2.1.1.228 0.0008 - guanine37 in Aquifex aeolicus tRNAArg(CCG) pH 7.6, 60°C 673850 2.1.1.228 0.0008 - guanine37 in yeast tRNAPhe(GAA) pH 7.6, 60°C 673850 2.1.1.228 0.0009 - guanine37 in Aquifex aeolicus tRNALeu(CAG) pH 7.6, 60°C 673850 2.1.1.228 0.0019 - guanine37 in Haloferax volcanii tRNATyr(GUA) pH 7.6, 60°C 673850 2.1.1.228 0.002 - guanine37 in Haloferax volcanii tRNACys(GCA) pH 7.6, 60°C 673850 2.1.1.228 0.0023 - guanine37 in Haloferax volcanii tRNATrp(CCA) pH 7.6, 60°C 673850 2.1.1.228 0.0033 - guanine37 in in Escherichia coli tRNALeu(CAG) pH 7.6, 60°C 673850 2.1.1.228 0.0000457 - guanine37 in yeast tRNAPhe possessing an A36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.0000546 - guanine37 in Escherichia coli tRNA1Leu pH 8.0, 37°C 661154 2.1.1.228 0.000075 - guanine37 in yeast tRNAAsp possessing a C36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.000244 - guanine37 in yeast tRNAAsp possessing a G36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.00058 - guanine37 in yeast tRNAAsp possessing an U36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.00059 - guanine37 in yeast tRNAAsp possessing an A36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.0028 - guanine37 in Escherichia coli tRNAPro pH 8.0, 37°C 712739 2.1.1.228 0.00546 - guanine37 in human mitochondrial tRNAPro pH 8.0, 37°C 661154 2.1.1.228 0.0057 - guanine37 in Escherichia coli tRNA1Leu pH 8.0, 37°C 661154 2.1.1.228 0.0073 - guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 37°C 712739 2.1.1.228 0.0086 - guanine37 in human mitochondrial tRNAPro pH 8.0, 37°C 661154 2.1.1.228 0.018 - guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.019 - guanine37 in yeast tRNAAsp possessing a G36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.046 - guanine37 in yeast tRNAAsp possessing an A36G37 sequence pH 8.0, 37°C 661154 2.1.1.228 0.00028 - guanine37 in Methanocaldococcus jannaschii tRNA(Cys) pH 8.0, 50°C 701123 2.1.1.228 0.003 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme D223A 711197 2.1.1.228 0.0046 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme G205A/G207A 711197 2.1.1.228 0.0072 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N225A 711197 2.1.1.228 0.0083 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme N265A 711197 2.1.1.228 0.003 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P226A 711197 2.1.1.228 0.0005 - S-adenosyl-L-methionine pH 8.0, 50°C, mutant enzyme P267A 711197 2.1.1.228 0.006 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme P267A 711197 2.1.1.228 0.0043 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme R144A 711197 2.1.1.228 0.0025 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, mutant enzyme Y176A 711197 2.1.1.228 0.0007 - guanine37 in Methanocaldococcus jannaschii tRNACys pH 8.0, 50°C, wild-type enzyme 711197 2.1.1.228 0.001 - S-adenosyl-L-methionine pH 8.0, 50°C, wild-type enzyme 711197 2.1.1.228 0.0012 - guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 52°C 712739 2.1.1.228 0.00082 - guanine37 in tRNAGlnCUG pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.001 - guanine37 in tRNAPro pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.0027 - guanine37 in tRNAGlnG36A pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.0044 - guanine37 in tRNAGlnCUG pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.008 - guanine37 in tRNALeu pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.027 - guanine37 in tRNAArg pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.064 - guanine37 in tRNAGlnCUG pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.067 - guanine37 in tRNAGlnCUG pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.114 - guanine37 in tRNAGlnG36U pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.184 - guanine37 in tRNAGlnG36C pH 8.0, temperature not specified in the publication 737174 2.1.1.228 0.0007 - S-adenosyl-L-homocysteine pH 8.5, 37°C 662010 2.1.1.228 0.0043 - guanine37 in tRNALeu(GAC) pH 8.5, 37°C 662010 2.1.1.228 0.0012 - guanine37 in tRNALeu(CAG) pH and temperature not specified in the publication 755697 2.1.1.228 0.0018 - S-adenosyl-L-methionine pH and temperature not specified in the publication 755697 2.1.1.228 additional information - additional information pre-steady-state and steady-state kinetic analysis of wild-type and mutant enzymes, the rate-determining step is product release from the enzyme, kinetic isotope effect, overview 737226 2.1.1.228 additional information - additional information pre-steady-state and steady-state kinetics, rapid burst phase followed by a slower and linear phase in reaction, single-turnover and from steady-state analysis, overview 720240 2.1.1.228 additional information - additional information pre-steady-state and steady-state kinetics, time-dependent linear reaction, overview. TrmD exhibits half-of-the-sites reactivity in which only one of the two active sites is functional at a given time 720240 2.1.1.228 additional information - additional information pre-steady-state and steady-state Michaelis-Menten kinetics, single turnover assays 757582 2.1.1.228 0.00042 - S-adenosyl-L-methionine recombinant enzyme, pH 7.3, 37°C 737226 2.1.1.228 0.00047 - guanine37 in tRNACys recombinant enzyme, pH 7.3, 37°C 737226 2.1.1.228 0.013 - guanine37 in tRNALeu recombinant mutant S88L, pH 8.0, 30°C 736428 2.1.1.228 0.0522 - guanine37 in tRNALeu recombinant mutant S88L, pH 8.0, 37°C 736428 2.1.1.228 0.0625 - guanine37 in tRNALeu recombinant mutant S88L, pH 8.0, 43°C 736428 2.1.1.228 0.0024 - guanine37 in tRNALeu recombinant wild-type enzyme, pH 8.0, 30°C 736428 2.1.1.228 0.0031 - guanine37 in tRNALeu recombinant wild-type enzyme, pH 8.0, 37°C 736428 2.1.1.228 0.0217 - guanine37 in tRNALeu recombinant wild-type enzyme, pH 8.0, 43°C 736428 2.1.1.228 additional information - additional information S-adenosyl-L-methionine and adenosine binding kinetics and kinetic analysis of enzyme reaction, overview 721029 2.1.1.228 additional information - additional information single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview 721035 2.1.1.228 additional information - additional information structure-guided kinetic analysis of TrmD mutants and tRNA variants, overview 737174 2.1.1.228 additional information - additional information wild-type and mutant enzymes pH-dependence of the single-turnover rate constant: the pH dependence of kobs in single-turnover analysis corresponds to proton ransfer during a slower process of induced fit, rather than the bond-breaking and bond-forming steps of methyl transfer, detailed analysis and overview 721026