1.6.2.2 additional information - additional information detailed analysis of biphasic rate of reduction of cytochrome b5 in membranes. The initial rapid phase is completed within 10 msec and over 90% of cytochrome b5 are reduced in 40 msec. Evaluation of data in terms of two-dimensional random walk model 672516 1.6.2.2 0.000007 - ferricytochrome b5 - 394205 1.6.2.2 0.000088 - ferricytochrome b5 - 394255 1.6.2.2 0.00016 - NADH enzyme from erythrocyte 394225 1.6.2.2 0.00029 - ferricytochrome c soluble isoform, pH 7, 37°C 765742 1.6.2.2 0.0003 - NADH H49E mutant enzyme 394254 1.6.2.2 0.00042 - ferricytochrome c membrane-bound isoform, pH 7, 37°C 765742 1.6.2.2 0.0006 - ferricyanide H49K mutant enzyme 394254 1.6.2.2 0.0006 - NADH enzyme from erythrocyte membrane 392811 1.6.2.2 0.0006 - NADH recombinant wild-type enzyme 394238 1.6.2.2 0.00064 - NADH G273 mutant enzyme 394254 1.6.2.2 0.0008 - ferricytochrome b5 - 394228 1.6.2.2 0.0008 - ferricytochrome b5 yeast cytochrome b5 394228 1.6.2.2 0.0008 - NADH - 394199 1.6.2.2 0.00084 - NADH DELTAF272 mutant enzyme 394254 1.6.2.2 0.00091 - NADH - 394209 1.6.2.2 0.001 - ferrocytochrome b5 mutant G179P, pH 7.0 671983 1.6.2.2 0.001 - NADPH cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein 394260 1.6.2.2 0.00104 - ferricyanide mutant enzyme P247L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.00104 - ferricyanide mutant P247L 699061 1.6.2.2 0.0011 - NADH - 394214 1.6.2.2 0.00114 - cytochrome b5 - 394199 1.6.2.2 0.00148 - ferricytochrome b5 S99A mutant enzyme 394259 1.6.2.2 0.0015 - ferricytochrome b5 calf cytochrome b5 394228 1.6.2.2 0.0015 - NADH recombinant wild-type enzyme 394254 1.6.2.2 0.0016 - ferricyanide pH 7.0, 25°C,T66V mutant enzyme 659255 1.6.2.2 0.0016 - NADH native enzyme 394254 1.6.2.2 0.0019 - NADH H49A mutant enzyme 394254 1.6.2.2 0.0019 - NADH S99T mutant enzyme 394259 1.6.2.2 0.002 - NADH - 685672 1.6.2.2 0.0021 - NADH R63K mutant enzyme 394259 1.6.2.2 0.0022 - ferricyanide H49E mutant enzyme 394254 1.6.2.2 0.0022 - ferricyanide pH 7.0, 25°C,T66A mutant enzyme 659255 1.6.2.2 0.0023 - NADH recombinant K110R mutant enzyme 394238 1.6.2.2 0.0025 - cytochrome b5 G273 mutant enzyme 394254 1.6.2.2 0.0025 - ferricyanide native enzyme 394254 1.6.2.2 0.0025 - ferricyanide pH 7.0, 25°C, wild type enzyme 659255 1.6.2.2 0.0025 - NADH - 394218 1.6.2.2 0.0026 - 2-hydroxyestradiol pH 7.8, 37°C 765854 1.6.2.2 0.0026 - ferricyanide H49Y mutant enzyme 394254 1.6.2.2 0.0027 - NADH H49Y mutant enzyme 394254 1.6.2.2 0.0028 - cytochrome b5 - 394194 1.6.2.2 0.0028 - ferricyanide H49A mutant enzyme 394254 1.6.2.2 0.0028 - ferricyanide recombinant wild-type enzyme 394254 1.6.2.2 0.0028 - NADH at pH 7.4 and 37°C 742559 1.6.2.2 0.0028 - NADH pH 7.0, 25°C,T66S mutant enzyme 659255 1.6.2.2 0.0028 - NADH pH 7.0, 25°C,T66V mutant enzyme 659255 1.6.2.2 0.0029 - NADH K97R mutant enzyme 394259 1.6.2.2 0.0029 - NADH pH 7.0, 25°C,T66A mutant enzyme 659255 1.6.2.2 0.003 - cytochrome b5 K110Q mutant enzyme 394258 1.6.2.2 0.003 - NADH F251Y mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.003 - NADH pH 7.0, wild type enzyme with cytochrome b5 as substrate 657966 1.6.2.2 0.003 - NADH Y65F mutant enzyme 394259 1.6.2.2 0.0031 - cytochrome b5 H49K mutant enzyme 394254 1.6.2.2 0.0031 - ferricyanide pH 7.0, 25°C,T66S mutant enzyme 659255 1.6.2.2 0.0031 - NADH pH 7.0, 25°C, wild type enzyme 659255 1.6.2.2 0.0031 - NADH recombinant wild-type enzyme 394259 1.6.2.2 0.0038 - NADH mutant Y93S, pH 7.0, 25°C 672018 1.6.2.2 0.004 - cytochrome b5 K110E mutant enzyme 394258 1.6.2.2 0.004 - ferricytochrome b5 wild-type, 25°C, pH 7.0 671595 1.6.2.2 0.0042 - ferricyanide - 394214 1.6.2.2 0.0042 - NADH Y65A mutant enzyme 394259 1.6.2.2 0.0044 - NADH K97A mutant enzyme 394259 1.6.2.2 0.0048 - NADH mutant P92A, pH 7.0, 25°C 672018 1.6.2.2 0.0049 - NADH mutant Y93D, pH 7.0, 25°C 672018 1.6.2.2 0.005 - ferricyanide K110R mutant enzyme 394258 1.6.2.2 0.005 - ferricytochrome b5 Y65A mutant enzyme 394259 1.6.2.2 0.005 - NADH D239S/F251Y mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.0052 - ferricyanide DELTAF272 mutant enzyme 394254 1.6.2.2 0.0052 - ferricyanide G273 mutant enzyme 394254 1.6.2.2 0.0053 - ferricytochrome b5 in the presence of 2 mM Ca2+ 394197 1.6.2.2 0.0057 - 9,10-phenanthrenequinone pH 7.8, 37°C 765854 1.6.2.2 0.0058 - ferricyanide mutant Y93S, pH 7.0, 25°C 672018 1.6.2.2 0.006 - ferricyanide mutant P275L, 25°C, pH 7.0 671595 1.6.2.2 0.006 - ferricyanide recombinant wild-type enzyme 394258 1.6.2.2 0.006 - ferricyanide wild-type 672788 1.6.2.2 0.006 - NADH D239E mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.006 - NADH mutant P275L, cosubstrate ferricyanide, 25°C, pH 7.0 671595 1.6.2.2 0.006 - NADH pH 7.0, wild type enzyme with ferricyanide as substrate 657966 1.6.2.2 0.006 - NADH recombinant enzyme 394247 1.6.2.2 0.006 - NADH wild type enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.006 - NADH wild-type 672788 1.6.2.2 0.006 - NADH wild-type, pH 7.0 671983 1.6.2.2 0.006 - NADH wild-type, pH 7.0, 25°C 672018 1.6.2.2 0.0062 - ferricytochrome b5 Y65F mutant enzyme 394259 1.6.2.2 0.0063 - NADH - 394228 1.6.2.2 0.0065 - NADH mutant P92G, pH 7.0, 25°C 672018 1.6.2.2 0.0066 - ferricyanide mutant Y93F, pH 7.0, 25°C 672018 1.6.2.2 0.0066 - ferricytochrome b5 recombinant wild-type enzyme 394238 1.6.2.2 0.0067 - NADH mutant Y93F, pH 7.0, 25°C 672018 1.6.2.2 0.0068 - ferricyanide mutant Y93W, pH 7.0, 25°C 672018 1.6.2.2 0.0069 - ferricytochrome b5 K97R mutant enzyme 394259 1.6.2.2 0.00693 - ferricytochrome b5 mutant P247L 699061 1.6.2.2 0.007 - ferricyanide mutant G179P, pH 7.0 671983 1.6.2.2 0.007 - ferricyanide mutant G179V, pH 7.0 671983 1.6.2.2 0.007 - ferricyanide mutant G75S 672788 1.6.2.2 0.007 - ferricyanide mutant V252M 672788 1.6.2.2 0.007 - ferricyanide pH 7.0, wild type enzyme 657966 1.6.2.2 0.007 - ferricyanide recombinant enzyme 394247 1.6.2.2 0.007 - ferricyanide wild-type, 25°C, pH 7.0 671595 1.6.2.2 0.007 - ferricytochrome c cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein 394260 1.6.2.2 0.007 - NADH pH 7.0, 25°C 671607 1.6.2.2 0.0071 - ferricyanide wild-type, pH 7.0, 25°C 672018 1.6.2.2 0.00723 - ferricyanide - 699061 1.6.2.2 0.00723 - ferricyanide wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.0073 - ferricytochrome b5 R63K mutant enzyme 394259 1.6.2.2 0.0073 - NADH mutant Y93A, pH 7.0, 25°C 672018 1.6.2.2 0.0074 - ferricyanide mutant P92A, pH 7.0, 25°C 672018 1.6.2.2 0.0077 - NADH mutant P92S, pH 7.0, 25°C 672018 1.6.2.2 0.0078 - ferricyanide mutant P92S, pH 7.0, 25°C 672018 1.6.2.2 0.008 - ferricyanide cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein 394260 1.6.2.2 0.008 - ferricyanide L148P mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.008 - ferricyanide mutant G179A, pH 7.0 671983 1.6.2.2 0.008 - ferricyanide mutant G179T, pH 7.0 671983 1.6.2.2 0.008 - ferricyanide mutant G75S/V252M 672788 1.6.2.2 0.008 - ferricyanide mutant P92G, pH 7.0, 25°C 672018 1.6.2.2 0.008 - ferricyanide P144L mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.008 - ferricyanide P144L/L148P mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.008 - ferricyanide pH 7.0, 25°C 671607 1.6.2.2 0.008 - ferricyanide pH 7.0, S127P mutant enzyme 657966 1.6.2.2 0.008 - ferricyanide wild type enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.008 - ferricyanide wild-type, pH 7.0 671983 1.6.2.2 0.008 - ferrocytochrome b5 mutant G179A, pH 7.0 671983 1.6.2.2 0.008 - methemoglobin-ferrocyanide complex - 394227 1.6.2.2 0.0083 - ferricyanide mutant Y93D, pH 7.0, 25°C 672018 1.6.2.2 0.0083 - ferricyanide mutant Y93H, pH 7.0, 25°C 672018 1.6.2.2 0.0086 - ferricyanide mutant Y93A, pH 7.0, 25°C 672018 1.6.2.2 0.0088 - cytochrome b5 native enzyme 394254 1.6.2.2 0.0088 - NADH mutant Y93H, pH 7.0, 25°C 672018 1.6.2.2 0.0089 - cytochrome b5 H49A mutant enzyme 394254 1.6.2.2 0.009 - cytochrome b5 K110A mutant enzyme 394258 1.6.2.2 0.009 - cytochrome b5 K110R mutant enzyme 394258 1.6.2.2 0.009 - ferricytochrome b5 - 394218 1.6.2.2 0.009 - NADH mutant G75S 672788 1.6.2.2 0.009 - NADPH D239T mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.0091 - cytochrome b5 recombinant wild-type enzyme 394254 1.6.2.2 0.0094 - NADH S99V mutant enzyme 394259 1.6.2.2 0.0098 - 1,4-Naphthoquinone pH 7.8, 37°C 765854 1.6.2.2 0.0099 - ferricytochrome b5 S99V mutant enzyme 394259 1.6.2.2 0.01 - cytochrome b5 recombinant wild-type enzyme 394258 1.6.2.2 0.01 - ferricyanide - 394227 1.6.2.2 0.01 - ferricytochome b5 L148P mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.01 - ferricytochrome b5 mutant Y93D, pH 7.0, 25°C 672018 1.6.2.2 0.01 - ferricytochrome b5 mutant Y93H, pH 7.0, 25°C 672018 1.6.2.2 0.01 - NADH H49K mutant enzyme 394254 1.6.2.2 0.0104 - ferricytochrome b5 recombinant wild-type enzyme 394259 1.6.2.2 0.01096 - ferricyanide mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.01096 - ferricyanide mutant P247A 699061 1.6.2.2 0.01097 - NADH ferricyanide as electron acceptor 699061 1.6.2.2 0.01097 - NADH wild type enzyme, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.011 - cytochrome b5 H49Y mutant enzyme 394254 1.6.2.2 0.011 - ferricytochrome b5 mutant Y93F, pH 7.0, 25°C 672018 1.6.2.2 0.011 - ferricytochrome b5 mutant Y93W, pH 7.0, 25°C 672018 1.6.2.2 0.011 - ferricytochrome b5 recombinant enzyme 394247 1.6.2.2 0.0112 - NADH in the presence of 2 mM Ca2+ 394197 1.6.2.2 0.0118 - ferricytochrome b5 S99T mutant enzyme 394259 1.6.2.2 0.012 - cytochrome b5 K110H mutant enzyme 394258 1.6.2.2 0.012 - ferricytochome b5 P144L mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.012 - ferricytochrome b5 mutant P275L, 25°C, pH 7.0 671595 1.6.2.2 0.012 - ferricytochrome b5 mutant P92G, pH 7.0, 25°C 672018 1.6.2.2 0.012 - ferricytochrome b5 mutant P92S, pH 7.0, 25°C 672018 1.6.2.2 0.012 - ferricytochrome b5 mutant Y93S, pH 7.0, 25°C 672018 1.6.2.2 0.012 - ferrocytochrome b5 pH 7.0, 25°C 671607 1.6.2.2 0.012 - NADH F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.013 - ferricyanide - 696575 1.6.2.2 0.013 - ferricytochome b5 P144L/L148P mutant enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.013 - ferricytochome b5 wild type enzyme, pH 7.0, 25°C 657675 1.6.2.2 0.013 - ferricytochrome b5 - 394249 1.6.2.2 0.013 - ferricytochrome b5 mutant V252M 672788 1.6.2.2 0.013 - ferricytochrome b5 pH 7.0, 25°C,T66S mutant enzyme 659255 1.6.2.2 0.013 - ferricytochrome b5 pH 7.0, wild type enzyme 657966 1.6.2.2 0.013 - ferricytochrome b5 wild-type 672788 1.6.2.2 0.013 - ferricytochrome b5 wild-type, pH 7.0, 25°C 672018 1.6.2.2 0.013 - ferrocytochrome b5 wild-type, pH 7.0 671983 1.6.2.2 0.0135 - NADH mutant enzyme P248L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.0135 - NADH mutant P248L, ferricyanide as electron acceptor 699061 1.6.2.2 0.01353 - ferricytochrome b5 wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.0136 - ferricyanide in the presence of 2 mM Ca2+ 394197 1.6.2.2 0.014 - ferricytochrome b5 - 685672 1.6.2.2 0.014 - ferricytochrome b5 mutant G75S 672788 1.6.2.2 0.014 - ferricytochrome b5 mutant G75S/V252M 672788 1.6.2.2 0.014 - ferricytochrome b5 mutant Y93A, pH 7.0, 25°C 672018 1.6.2.2 0.014 - ferricytochrome b5 pH 7.0, S127P mutant enzyme 657966 1.6.2.2 0.0142 - ferricytochrome b5 recombinant K110A mutant enzyme 394238 1.6.2.2 0.0143 - ferricytochrome b5 - 394214 1.6.2.2 0.0144 - NADH - 394241 1.6.2.2 0.015 - cytochrome b5 - 394227 1.6.2.2 0.015 - ferricytochrome b5 mutant P92A, pH 7.0, 25°C 672018 1.6.2.2 0.0155 - NADH mutant enzyme P249L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.0155 - NADH mutant P249L, ferricyanide as electron acceptor 699061 1.6.2.2 0.01563 - ferricyanide mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.01563 - ferricyanide mutant P248A 699061 1.6.2.2 0.01568 - ferricyanide mutant enzyme P248L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.01568 - ferricyanide mutant P248L 699061 1.6.2.2 0.0161 - ferricytochrome b5 K97A mutant enzyme 394259 1.6.2.2 0.0167 - ferricytochrome b5 recombinant K110R mutant enzyme 394238 1.6.2.2 0.017 - cytochrome b5 DELTAF272 mutant enzyme 394254 1.6.2.2 0.017 - ferricyanide - 394249 1.6.2.2 0.017 - NADH D239S mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.017 - NADH mutant G75S/V252M 672788 1.6.2.2 0.01713 - NADH mutant enzyme P247A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.01713 - NADH mutant P247A, ferricyanide as electron acceptor 699061 1.6.2.2 0.01735 - ferricytochrome b5 - 699061 1.6.2.2 0.0185 - ferricytochrome b5 R63Q mutant enzyme 394259 1.6.2.2 0.0195 - NADH - 394197 1.6.2.2 0.02 - ferricytochrome b5 enzyme from erythrocyte membrane 392811 1.6.2.2 0.02 - ferricytochrome b5 enzyme from eythrocyte membrane 394213 1.6.2.2 0.02 - ferricytochrome b5 pH 7.0, 25°C, wild type enzyme 659255 1.6.2.2 0.0207 - ferricytochrome b5 mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.021 - NADH R63Q mutant enzyme 394259 1.6.2.2 0.022 - NADPH D239S/F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.0231 - ferricyanide - 394197 1.6.2.2 0.024 - ferricytochrome b5 - 394255 1.6.2.2 0.02407 - ferricytochrome b5 mutant P247A 699061 1.6.2.2 0.02407 - NADH mutant enzyme P248A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.02407 - NADH mutant P248A, ferricyanide as electron acceptor 699061 1.6.2.2 0.02467 - NADH mutant enzyme P247L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.02467 - NADH mutant P247L, ferricyanide as electron acceptor 699061 1.6.2.2 0.025 - NADH mutant G179A, pH 7.0 671983 1.6.2.2 0.025 - NADH pH 7.0, S127P mutant enzyme with cytochrome b5 as substrate 657966 1.6.2.2 0.025 - NADPH D239S/F251Y mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.026 - NADH S99A mutant enzyme 394259 1.6.2.2 0.02646 - ferricyanide mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.02646 - ferricyanide mutant P249A 699061 1.6.2.2 0.0285 - ferricytochrome b5 recombinant K110M mutant enzyme 394238 1.6.2.2 0.02928 - NADH mutant enzyme P249A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.02928 - NADH mutant P249A, ferricyanide as electron acceptor 699061 1.6.2.2 0.03 - cytochrome b5 H49E mutant enzyme 394254 1.6.2.2 0.03309 - ferricyanide mutant enzyme P249L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.03309 - ferricyanide mutant P249L 699061 1.6.2.2 0.03434 - ferricytochrome b5 mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.0349 - ferricytochrome b5 - 394197 1.6.2.2 0.035 - ferricytochrome b5 enzyme from liver microsome membrane, solubilized with Triton X-100 394213 1.6.2.2 0.0362 - cytochrome b5 L125A mutant enzyme 394251 1.6.2.2 0.0384 - ferricyanide - 394216 1.6.2.2 0.04 - ferricytochrome b5 enzyme from eythrocyte cytosol 394213 1.6.2.2 0.04148 - ferricytochrome b5 mutant P248A 699061 1.6.2.2 0.0419 - aquacobalamin - 394241 1.6.2.2 0.042 - cytochrome b5 L41A mutant enzyme 394251 1.6.2.2 0.0421 - ferricytochrome b5 R63A mutant enzyme 394259 1.6.2.2 0.043 - ferrocytochrome b5 mutant G179T, pH 7.0 671983 1.6.2.2 0.044 - NADH - 394249 1.6.2.2 0.044 - NADPH D239T/F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.04481 - ferricytochrome b5 mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C 699061 1.6.2.2 0.045 - ferricytochrome b5 enzyme from liver microsome membrane, cathepsin D solubilized 394213 1.6.2.2 0.0478 - NADH - 394216 1.6.2.2 0.05 - ferricyanide K110H mutant enzyme 394258 1.6.2.2 0.052 - NADH mutant V252M 672788 1.6.2.2 0.05238 - ferricytochrome b5 mutant P249A 699061 1.6.2.2 0.054 - NADH mutant Y93W, pH 7.0, 25°C 672018 1.6.2.2 0.055 - ferricytochrome b5 mutant P249L 699061 1.6.2.2 0.055 - NADH pH 7.0, S127P mutant enzyme with ferricyanide as substrate 657966 1.6.2.2 0.06 - NADH - 394194 1.6.2.2 0.077 - 2-methyl-1,4-naphthoquinone pH 7.8, 37°C 765854 1.6.2.2 0.08199 - ferricytochrome b5 mutant P248L 699061 1.6.2.2 0.09 - ferricyanide - 394194 1.6.2.2 0.094 - NADPH D239E mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.104 - NADH R63A mutant enzyme 394259 1.6.2.2 0.107 - ferrocytochrome b5 mutant G179V, pH 7.0 671983 1.6.2.2 0.111 - ferricytochrome b5 pH 7.0, 25°C,T66V mutant enzyme 659255 1.6.2.2 0.119 - NADH D239T mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.121 - NADH recombinant K110A mutant enzyme 394238 1.6.2.2 0.125 - ferricytochrome b5 pH 7.0, 25°C,T66A mutant enzyme 659255 1.6.2.2 0.138 - NADPH F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.22 - N-Hydroxy-4-aminobiphenyl pH 7.4 673060 1.6.2.2 0.224 - NADH D239S/F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.25 - N-hydroxy-2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine pH 7.4 673060 1.6.2.2 0.268 - NADPH D239S mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.27 - Nitrofurantoin pH 7.8, 37°C 765854 1.6.2.2 0.328 - oxidized 2,6-dichlorophenolindophenol at pH 6.4 and 25°C 742312 1.6.2.2 0.345 - ferricyanide at pH 6.4 and 25°C 742312 1.6.2.2 0.36 - dapsone hydroxylamine pH 7.4 659784 1.6.2.2 0.36 - sulfamethoxazole hydroxylamine pH 7.4 659784 1.6.2.2 0.37 - ferricyanide K110E mutant enzyme 394258 1.6.2.2 0.375 - NADPH mutant G179V, pH 7.0 671983 1.6.2.2 0.38 - ferricyanide K110A mutant enzyme 394258 1.6.2.2 0.507 - NADPH mutant G179T, pH 7.0 671983 1.6.2.2 0.512 - NADH D239T/F251R mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.595 - NADH mutant G179P, pH 7.0 671983 1.6.2.2 0.617 - NADPH F251Y mutant enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.63 - benzamidoxime pH 7.4 659784 1.6.2.2 0.662 - NADH mutant G179T, pH 7.0 671983 1.6.2.2 0.672 - NADH recombinant K110M mutant enzyme 394238 1.6.2.2 0.76 - ferricyanide K110Q mutant enzyme 394258 1.6.2.2 0.83 - oxidized 2,6-dichlorophenolindophenol - 394249 1.6.2.2 0.924 - NADPH wild type enzyme, pH 7.0, 25°C 657949 1.6.2.2 0.924 - NADPH wild-type, pH 7.0 671983 1.6.2.2 1.077 - NADH mutant G179V, pH 7.0 671983 1.6.2.2 1.36 - NADPH mutant G179A, pH 7.0 671983 1.6.2.2 2.17 - NADH - 394252 1.6.2.2 2.317 - NADPH mutant G179P, pH 7.0 671983 1.6.2.2 2.34 - NADPH - 394252 1.6.2.2 2.623 - NADH wild-type, cosubstrate ferricyanide, 25°C, pH 7.0 671595 1.6.2.2 3.32 - NADH - 394252 1.6.2.2 3.85 - ferricyanide - 394252 1.6.2.2 4.35 - ferricyanide - 394252 1.6.2.2 7.69 - NADPH - 394252 1.6.2.2 14 - ferricytochrome b5 - 394249 1.6.2.2 0.0004 0.0005 NADH lysosome- and detergent-solubilized enzyme 394212 1.6.2.2 0.0006 0.0007 NADH enzyme from erythrocyte and liver 394213 1.6.2.2 0.03 0.04 cytochrome b5 lysosome- and detergent-solubilized enzyme 394212 1.6.2.2 0.025 0.089 testosterone depending on phosphate concentration 394211