1.14.14.16 additional information - additional information calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview 726997 1.14.14.16 additional information - additional information Pre-steady-state and steady-state binding kinetics with 17alpha-hydroxyprogesterone, stopped-flow spectroscopic measurements, overview 727898 1.14.14.16 additional information - additional information reaction kinetic, overview 715690 1.14.14.16 0.00021 - progesterone recombinant enzyme, at pH 7.4 and 37°C 734302 1.14.14.16 0.00021 - progesterone wild-type, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.00039 - progesterone mutant R409C, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.00044 - 17alpha-hydroxyprogesterone mutant R409C, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.00044 - 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C 734302 1.14.14.16 0.00046 - 17alpha-hydroxyprogesterone mutant T296N, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.00048 - progesterone pH 7.4, 37°C, mutant V359A 726962 1.14.14.16 0.0005 - progesterone recombinant enzyme, at pH 7.4 and 37°C 734302 1.14.14.16 0.00061 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, mutant V359G 726962 1.14.14.16 0.00072 - progesterone mutant enzyme D322G, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.00072 - progesterone wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.00079 - progesterone mutant enzyme A265V, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.00082 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, mutant V359A 726962 1.14.14.16 0.001 - progesterone pH 7.4, 37°C, mutant V470A 726962 1.14.14.16 0.0011 - progesterone mutant T296N, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.0011 - progesterone pH 7.4, 37°C, wild-type enzyme 726962 1.14.14.16 0.0012 - progesterone mutant G292C, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0012 - progesterone mutant P31L, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.00135 - 17alpha-hydroxyprogesterone mutant enzyme D322G, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.00138 - 17alpha-hydroxyprogesterone wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.0014 - 17alpha-hydroxyprogesterone mutant V282L, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0015 - 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C 734302 1.14.14.16 0.0015 - 17alpha-hydroxyprogesterone wild-type, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.0015 - progesterone mutant enzyme P453S 699170 1.14.14.16 0.0015 - progesterone pH 7.4, 37°C, mutant V470A/I471A 726962 1.14.14.16 0.0015 - progesterone wild type enzyme 699170 1.14.14.16 0.0016 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, mutant V470A/I471A 726962 1.14.14.16 0.0016 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, wild-type enzyme 726962 1.14.14.16 0.0016 - 17alpha-hydroxyprogesterone wild type enzyme 699170 1.14.14.16 0.0017 - 17alpha-hydroxyprogesterone mutant L108R, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0017 - 17alpha-hydroxyprogesterone mutant P31L, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0018 - progesterone mutant I172N, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.00181 - 17alpha-hydroxyprogesterone mutant enzyme A265V, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C 698190 1.14.14.16 0.0019 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, wild-type CYP21 727898 1.14.14.16 0.002 - 17alpha-hydroxyprogesterone mutant enzyme P453S 699170 1.14.14.16 0.0024 - 17alpha-hydroxyprogesterone mutant W303R, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0024 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, CYP21 mutant T241R/L442A 727898 1.14.14.16 0.0024 - progesterone mutant enzyme K121Q 699170 1.14.14.16 0.0025 - 17alpha-hydroxyprogesterone pH 7.4, 37°C, mutant V470A 726962 1.14.14.16 0.0026 - 17alpha-hydroxyprogesterone mutant enzyme K121Q 699170 1.14.14.16 0.0027 - progesterone mutant L108R, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0032 - progesterone pH 7.4, 37°C, mutant V359G 726962 1.14.14.16 0.0036 - 17alpha-hydroxyprogesterone mutant G292C, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0038 - 17-hydroxyprogesterone head kidney microsomes 285444 1.14.14.16 0.0038 - progesterone mutant V282L, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0046 - 17alpha-hydroxyprogesterone mutant G292S, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0047 - progesterone mutant G292S, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0049 - 17alpha-hydroxyprogesterone mutant I172N, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0055 - 17-hydroxyprogesterone - 285446 1.14.14.16 0.0055 - progesterone - 285446 1.14.14.16 0.0055 - progesterone mutant G65E, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.0073 - 17alpha-hydroxyprogesterone mutant P31Q, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0096 - progesterone mutant P31Q, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.0108 - 17alpha-hydroxyprogesterone 37°C, pH 7.4, presence of 0.002% Cymal 5 671760 1.14.14.16 0.0129 - progesterone 37°C, pH 7.4, presence of 0.002% Cymal 5 671760 1.14.14.16 0.013 - 17alpha-hydroxyprogesterone mutant G65E, pH not specified in the publication, temperature not specified in the publication 745363, 745776 1.14.14.16 0.022 - 17alpha-hydroxyprogesterone mutant R357W, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.022 - progesterone mutant R357W, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 0.031 - 17-hydroxyprogesterone liver microsomes 285444 1.14.14.16 0.11 - progesterone mutant W303R, pH not specified in the publication, temperature not specified in the publication 745363 1.14.14.16 2.3 - 17-hydroxy-progesterone 37°C, mutant P482S 659570 1.14.14.16 3 - progesterone 37°C, mutant P482S 659570 1.14.14.16 6.4 - progesterone 37°C, wild-type 659570 1.14.14.16 8.5 - 17-hydroxy-progesterone 37°C, wild-type 659570 1.14.14.16 9.2 - 17-hydroxy-progesterone 37°C, mutant A15T 659570 1.14.14.16 9.2 - progesterone 37°C, mutant A15T 659570