1.1.1.82 additional information - additional information Michaelis-Menten kinetics 740926 1.1.1.82 0.000019 - NADP+ pH and temperature not specified in the publication 740926 1.1.1.82 0.00012 - (S)-malate pH and temperature not specified in the publication 740926 1.1.1.82 0.011 - NADPH - 287420 1.1.1.82 0.0114 - NADPH mutant enzyme D201N 287428 1.1.1.82 0.014 - oxaloacetate enzyme from intermembrane space 286653 1.1.1.82 0.0145 - (S)-malate - 287409 1.1.1.82 0.017 - oxaloacetate mutant enzyme C23A/C28A/C364A/C376A, oxidized form 287419 1.1.1.82 0.018 - oxaloacetate - 287399 1.1.1.82 0.019 - (S)-malate pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme 696461 1.1.1.82 0.019 - NADP+ pH and temperature not specified in the publication 740926 1.1.1.82 0.019 - oxaloacetate recombinant enzyme cloned from expression vector pJ2PCC2 287426 1.1.1.82 0.02 - NADPH pH and temperature not specified in the publication 740926 1.1.1.82 0.02 - oxaloacetate mutant enzyme C23A, reduced form 287419 1.1.1.82 0.021 - NADPH mutant enzyme C23A/C28A and C23A/C28A/C376A, oxidized form 287419 1.1.1.82 0.021 - oxaloacetate recombinant enzyme cloned from expression vector pJ2PCC1 287426 1.1.1.82 0.022 - oxaloacetate mutant enzyme C23A/C28A, reduced form 287419 1.1.1.82 0.022 - oxaloacetate mutant enzyme DELTAN 287421 1.1.1.82 0.023 - NADP+ pH and temperature not specified in the publication 740926 1.1.1.82 0.023 - NADPH mutant enzyme C23A/C28A/C206A/C364A, oxidized form 287419 1.1.1.82 0.023 - NADPH mutant enzyme D201A 287428 1.1.1.82 0.024 - NADPH - 287393 1.1.1.82 0.024 - NADPH mutant enzyme C23A/C28A, reduced form 287419 1.1.1.82 0.024 - oxaloacetate mutant enzyme C23A/C28A/C206A/C376A, oxidized form 287419 1.1.1.82 0.025 - oxaloacetate mutant enzyme C23A/C28A/C206A/C364A, oxidized form and mutant DELTAN, lacking the 45 N-terminal residues 287419 1.1.1.82 0.026 - NADPH mutant enzyme C23A/C28A/C364A and C23A/C28A/C364A/C376A, oxidized form 287419 1.1.1.82 0.027 - oxaloacetate wild-type enzyme, reduced form and mutant enzyme C23A/C28A/C364A, oxidized form 287419 1.1.1.82 0.028 - NADPH mutant enzyme C23A/C28A/C206A/C376A, oxidized form 287419 1.1.1.82 0.028 - NADPH mutant enzyme C64S/C69S 287421 1.1.1.82 0.028 - NADPH wild-type enzyme and mutant C28A, reduced enzyme form 287419 1.1.1.82 0.028 - oxaloacetate mutant enzyme C23A/C28A/C206A, reduced enzyme form and mutant enzyme C23A/C28A/C376A, oxidized form 287419 1.1.1.82 0.029 - NADP+ - 287420 1.1.1.82 0.029 - NADPH mutant enzyme C207A/E387Q, oxidized form 287422 1.1.1.82 0.029 - NADPH mutant enzyme lacking the N-terminal 45 residues, reduced form 287419 1.1.1.82 0.03 - NADPH mutant enzyme C23A, reduced form 287419 1.1.1.82 0.03 - NADPH recombinant enzyme cloned from expression vector pJ2PCC1 287426 1.1.1.82 0.03 - oxaloacetate pH and temperature not specified in the publication 740926 1.1.1.82 0.031 - NADPH mutant enzyme C29S/C207A/E387Q, oxidized enzyme form 287422 1.1.1.82 0.033 - NADPH mutant enzyme C23A/C28A/C206A, oxidized form and mutant DELTAN lacking the N-terminal 45 residues, reduced form 287419 1.1.1.82 0.035 - NADPH enzyme from intermembrane space 286653 1.1.1.82 0.035 - NADPH mutant enzyme C29S/C207A/DELTAEV, oxidized form 287422 1.1.1.82 0.036 - NADPH mutant enzyme C23A/C28A/C206A, reduced form 287419 1.1.1.82 0.037 - NADPH mutant C69S 287421 1.1.1.82 0.037 - oxaloacetate enzyme from mitochondrial matrix 286653 1.1.1.82 0.037 - oxaloacetate mutant enzyme C64S 287421 1.1.1.82 0.038 - NADPH mutant enzyme C64S 287421 1.1.1.82 0.039 - NADPH - 287409, 287419 1.1.1.82 0.039 - NADPH mutant enzyme DELTAN 287421 1.1.1.82 0.039 - NADPH reduced native enzyme 287392, 287405 1.1.1.82 0.04 - oxaloacetate wild-type enzyme 287428 1.1.1.82 0.041 - oxaloacetate wild-type enzyme 287421 1.1.1.82 0.042 - NADPH wild-type enzyme 287421 1.1.1.82 0.042 - oxaloacetate mutant C69S 287421 1.1.1.82 0.042 - oxaloacetate reduced truncated enzyme 287392 1.1.1.82 0.043 - NADPH recombinant enzyme cloned from expression vector pJCPCC2 287426 1.1.1.82 0.044 - NADPH mutant enzyme C29S/E387Q, reduced form 287422 1.1.1.82 0.044 - oxaloacetate mutant enzyme C64S/C69S 287421 1.1.1.82 0.0446 - NADPH wild-type enzyme 287428 1.1.1.82 0.045 - NADP+ - 287399 1.1.1.82 0.046 - NADPH mutant enzyme C29S/E387Q, reduced form 287422 1.1.1.82 0.046 - NADPH oxidized truncated enzyme 287392 1.1.1.82 0.047 - NADPH mutant enzyme C207A/DELTAEV, oxidized form 287422 1.1.1.82 0.048 - oxaloacetate - 287409 1.1.1.82 0.048 - oxaloacetate reduced native enzyme 287392, 287405 1.1.1.82 0.049 - NADPH mutant enzyme C29S/DELTAEV, oxidized form 287422 1.1.1.82 0.05 - NADPH - 287399 1.1.1.82 0.05 - oxaloacetate mutant enzyme C39S/E387Q, reduced form 287422 1.1.1.82 0.054 - oxaloacetate mutant enzyme C207A/DELTAEV, reduced form 287422 1.1.1.82 0.056 - oxaloacetate cosubstrate NADPH 287393 1.1.1.82 0.056 - oxaloacetate mutant enzyme C29S/E387Q, reduced form 287422 1.1.1.82 0.06 - NADPH mutant enzyme C207A/DELTAEV, and mutant enzyme C29S/C207S/DELTAEV, reduced forms 287422 1.1.1.82 0.061 - oxaloacetate cosubstrate NADH 287393 1.1.1.82 0.062 - oxaloacetate - 287420 1.1.1.82 0.063 - NADP+ - 287409 1.1.1.82 0.063 - NADPH mutant enzyme C29S/DELTAEV, reduced form 287422 1.1.1.82 0.066 - NADPH enzyme from mitochondrial matrix 286653 1.1.1.82 0.066 - NADPH mutant enzyme C29S/C207A/E387Q, reduced form and mutant enzyme C29S/C207A/DELTAEV, reduced form 287422 1.1.1.82 0.07 - oxaloacetate mutant enzyme C29S/DELTAEV, reduced form 287422 1.1.1.82 0.073 - NADP+ cosubstrate malate 287393 1.1.1.82 0.077 - oxaloacetate reduced enzyme, + 200 mM guanidine 287405 1.1.1.82 0.086 - oxaloacetate mutant enzyme C29S/C207A/E387Q, reduced form 287422 1.1.1.82 0.089 - NADPH reduced truncated enzyme 287392 1.1.1.82 0.094 - oxaloacetate mutant enzyme C29S/C207A, reduced form 287422 1.1.1.82 0.095 - NADPH reduced enzyme, + 200 mM guanidine 287405 1.1.1.82 0.105 - oxaloacetate mutant enzyme C23A/C28A/C206A, oxidized form 287419 1.1.1.82 0.11 - oxaloacetate - 287403 1.1.1.82 0.115 - NADPH mutant enzyme C29S/C207A, reduced form 287422 1.1.1.82 0.12 - (S)-malate pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme 696461 1.1.1.82 0.13 - NADP+ pH and temperature not specified in the publication 740926 1.1.1.82 0.2 - (2S,3R)-tartrate pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme 696461 1.1.1.82 0.22 - NADPH oxidized enzyme, + 250 mM guanidine 287405 1.1.1.82 0.26 - (S)-malate pH and temperature not specified in the publication 740926 1.1.1.82 0.32 - oxaloacetate oxidized enzyme, + 250 mM guanidine 287405 1.1.1.82 0.36 - NADPH - 287403 1.1.1.82 0.4 - (S)-malate pH and temperature not specified in the publication 740926 1.1.1.82 0.447 - oxaloacetate mutant enzyme C207A/DELTAEV, oxidized form 287422 1.1.1.82 0.472 - NADPH mutant enzyme lacking the N-terminal 45 residues, oxidized form 287419 1.1.1.82 0.578 - oxaloacetate mutant enzyme C29S/DELTAEV, oxidized form 287422 1.1.1.82 0.583 - oxaloacetate mutant enzyme C29S/E387Q, oxidized form 287422 1.1.1.82 0.8 - NAD+ cosubstrate malate 287393 1.1.1.82 0.83 - NADH cosubstrate oxaloacetate 287393 1.1.1.82 0.894 - oxaloacetate mutant enzyme C29S/C207A/E387Q, oxidized form 287422 1.1.1.82 0.931 - oxaloacetate mutant enzyme C29S/C207A/DELTAEV, oxidized form 287422 1.1.1.82 1.2 - (2S,3S)-tartrate pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme 696461 1.1.1.82 1.2 - oxaloacetate oxidized truncated enzyme 287392 1.1.1.82 1.8 - oxaloacetate mutant enzyme D201N 287428 1.1.1.82 2 - (S)-malate - 287420 1.1.1.82 2 3 (2S,3S)-tartrate pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme 696461 1.1.1.82 3 - oxaloacetate mutant enzyme D201A 287428 1.1.1.82 5 - (S)-malate mutant enzyme D201A 287428 1.1.1.82 5.8 - (2S,3R)-tartrate pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme 696461 1.1.1.82 10.2 - (S)-malate mutant enzyme D201N 287428 1.1.1.82 12 - (S)-malate wild-type enzyme 287428 1.1.1.82 24 - (S)-malate - 287399 1.1.1.82 29 - (S)-malate cosubstrate NAD+ 287393 1.1.1.82 32 - (S)-malate cosubstrate NADP+ 287393