1.1.1.270 0.00012 - NADPH substrate: 5alpha-dihydrotestosterone, DHT 286478 1.1.1.270 0.001 - NADPH pH 7.4, 25°C, wild-type enzyme 724124 1.1.1.270 0.0023 - 5beta-pregnane-21-ol-3,20-dione pH 7.4, 25°C, mutant L116F 724124 1.1.1.270 0.0024 - 5beta-pregnane-21-ol-3,20-dione pH 7.4, 25°C, wild-type enzyme 724124 1.1.1.270 0.003 - NADPH substrate: DELTA7-chondrillastenone 286474 1.1.1.270 0.0035 - 5beta-dihydrotestosterone pH 7.4, 25°C, wild-type enzyme 724124 1.1.1.270 0.0037 - dehydroepiandrosterone pH 7.4, 27°C, wild-type enzyme 763397 1.1.1.270 0.004 - NADPH substrate: cycloeucalenone 286474 1.1.1.270 0.0043 - 17beta-hydroxy-5alpha-androstan-3-one pH 7.0, 37°C, spectrophotometric analysis, formation of 3beta-androstanediol or 3alpha-androstanediol 656200 1.1.1.270 0.0072 - 5beta-dihydrotestosterone pH 7.4, 25°C, mutant L116F 724124 1.1.1.270 0.0107 - dehydroepiandrosterone pH 7.4, 27°C, chimeric 195P-1 mutant 763397 1.1.1.270 0.011 - Pyridine-3-aldehyde pH 7.4, 25°C, wild-type enzyme and mutant F303Q 724124 1.1.1.270 0.0124 - dehydroepiandrosterone pH 7.4, 27°C, chimeric S194G-1 mutant 763397 1.1.1.270 0.013 - 5beta-pregnane-21-ol-3,20-dione pH 7.4, 25°C, mutant F303Q 724124 1.1.1.270 0.017 - 5beta-dihydrotestosterone pH 7.4, 25°C, mutant F303Q 724124 1.1.1.270 0.02 - 5beta-dihydrotestosterone pH 7.4, 25°C, mutant M304S 724124 1.1.1.270 0.0205 - NAD+ pH 7.4, 27°C, chimeric 195P-1 mutant 763397 1.1.1.270 0.022 - Pyridine-3-aldehyde pH 7.4, 25°C, mutant L116F 724124 1.1.1.270 0.023 - 5beta-pregnane-21-ol-3,20-dione pH 7.4, 25°C, mutant M304S 724124 1.1.1.270 0.0244 - NAD+ pH 7.4, 27°C, chimeric S194G-1 mutant 763397 1.1.1.270 0.0341 - NAD+ pH 7.4, 27°C, wild-type enzyme 763397 1.1.1.270 0.039 - 5beta-dihydrotestosterone pH 7.4, 25°C, mutant F303Q/M304S 724124 1.1.1.270 0.039 - Pyridine-3-aldehyde pH 7.4, 25°C, mutant M304S 724124 1.1.1.270 0.048 - Pyridine-3-aldehyde pH 7.4, 25°C, mutant F303Q/M304S 724124 1.1.1.270 0.05 - 5beta-pregnane-21-ol-3,20-dione pH 7.4, 25°C, mutant F303Q/M304S 724124 1.1.1.270 0.067 - cycloartenone - 286474 1.1.1.270 0.078 - 4alpha-methyl-5alpha-cholest-7-en-3-one solubilized enzyme 286472 1.1.1.270 0.085 - 24-methylenecycloartanone - 286474 1.1.1.270 0.1 - cyclolaudenone - 286474 1.1.1.270 0.162 - 24-methylenelophenone - 286474 1.1.1.270 0.18 - methylglyoxal pH 7.4, 25°C, mutant L116F 724124 1.1.1.270 0.209 - methylglyoxal pH 7.4, 25°C, mutant M304S 724124 1.1.1.270 0.22 - cholestanone - 286474 1.1.1.270 0.22 - cycloeucalenone - 286474 1.1.1.270 0.222 - methylglyoxal pH 7.4, 25°C, wild-type enzyme 724124 1.1.1.270 0.236 - 4alpha-methyl-5alpha-cholest-7-en-3-one microsomal-bound enzyme 286472 1.1.1.270 0.245 - DELTA7-chondrillastenone - 286474 1.1.1.270 0.25 - methylglyoxal pH 7.4, 25°C, mutant F303Q/M304S 724124 1.1.1.270 0.253 - methylglyoxal pH 7.4, 25°C, mutant F303Q 724124 1.1.1.270 0.26 - obtusifolione - 286474 1.1.1.270 0.283 - isodeoxycholic acid coenzyme: NADP+, pH 9.5, temperature not specified in the publication 760397 1.1.1.270 0.42 - DELTA7-cholestenone - 286474 1.1.1.270 0.47 - 3-oxodeoxycholic acid coenzyme: NADPH, pH 6.5, temperature not specified in the publication 760397