1.1.1.149 additional information - additional information kinetics 668428 1.1.1.149 additional information - additional information rapid activity decrease upon homogenization 285997 1.1.1.149 0.0002 - NADPH 20-oxo and 20alpha-hydroxy-steroids, pH 7.0 285998 1.1.1.149 0.0002 - NADPH wild type, pH 7.5, 37°C 656526 1.1.1.149 0.0004 - 5alpha-pregnane-3alpha,20alpha-diol mutant L308V, pH 7.4, 25°C 711423 1.1.1.149 0.0005 - 5alpha-pregnan-3,20-dione at pH 7.4 and 37°C 740126 1.1.1.149 0.0006 - isatin at pH 7.4 and 37°C 740126 1.1.1.149 0.0006 - progesterone at pH 7.4 and 37°C 740126 1.1.1.149 0.0006 - progesterone NADPH, intact cells 285997 1.1.1.149 0.0007 - 5alpha-pregnan-20alpha-ol-3-one pH 7.0, 25°C 655220 1.1.1.149 0.0007 - NADP+ spectrophotometric, pH 6.8 285983 1.1.1.149 0.0008 - 5alpha-pregnan-3alpha,20alpha-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.00087 - NADP+ HSD2 285978 1.1.1.149 0.00088 - NADP+ fluorometric, pH 7.8 285983 1.1.1.149 0.0009 - 5alpha-pregnane-3alpha,20alpha-diol pH 7.0, 25°C 655220 1.1.1.149 0.0009 - 5alpha-pregnane-3alpha,20alpha-diol wild-type, pH 7.4, 25°C 711423 1.1.1.149 0.0009 - Nonanal at pH 7.4 and 37°C 740126 1.1.1.149 0.001 - 2-tert-butyl-1,4-benzoquinone at pH 7.4 and 37°C 740126 1.1.1.149 0.001 - 4-androstene-3,17-dione at pH 7.4 and 37°C 740126 1.1.1.149 0.001 - 4-pregnen-3alpha-ol-20-one at pH 7.4 and 37°C 740126 1.1.1.149 0.001 - 5beta-pregnan-20alpha-ol-3-one pH 7.0, 25°C 655220 1.1.1.149 0.001 - 5beta-pregnan-3,20-dione at pH 7.4 and 37°C 740126 1.1.1.149 0.001 - 5beta-Pregnan-3alpha-ol-20-one at pH 7.4 and 37°C 740126 1.1.1.149 0.00102 - 20alpha-hydroxy-4-pregnen-3-one spectrophotometric, pH 6.8 285983 1.1.1.149 0.00105 - NADP+ HSD1 285978 1.1.1.149 0.0011 - 5alpha-pregnane-3,20-dione pH 7.0, 25°C 655220 1.1.1.149 0.0011 - estrone at pH 7.4 and 37°C 740126 1.1.1.149 0.00117 - 20alpha-hydroxyprogesterone wild type, pH 7.5, 37°C 656526 1.1.1.149 0.0012 - 4-Nitroacetophenone at pH 7.4 and 37°C 740126 1.1.1.149 0.0012 - 5alpha-pregnane-21-ol-3,20-dione pH 7.0, 25°C 655220 1.1.1.149 0.0012 - 5beta-pregnane-3,20-dione pH 7.0, 25°C 655220 1.1.1.149 0.0013 - 17beta-estradiol at pH 7.4 and 37°C 740126 1.1.1.149 0.0013 - 5alpha-pregnan-3alpha-ol-20-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0013 - 5beta-pregnan-20alpha-ol-3-one pH 7.0, 25°C 655220 1.1.1.149 0.0013 - n-octyl acetoacetate - 285999 1.1.1.149 0.0013 - progesterone pH 7.0, 25°C 655220 1.1.1.149 0.00138 - NADPH spectrophotometric, pH 6.8 285983 1.1.1.149 0.0014 - 5beta-Androstan-3alpha,17beta-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.0015 - 5beta-pregnane-3alpha,20alpha-diol pH 7.0, 25°C 655220 1.1.1.149 0.0015 - n-hexyl acetoacetate - 285999 1.1.1.149 0.0016 - n-octyl acetoacetate - 285999 1.1.1.149 0.00162 - 20alpha-hydroxy-4-pregnen-3-one fluorometric, pH 7.8 285983 1.1.1.149 0.0017 - 5beta-Androstan-3alpha-ol-17-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0018 - 4-oxo-2-nonenal at pH 7.4 and 37°C 740126 1.1.1.149 0.00186 - thionicotinamide adenine dinucleotide phosphate NADP analog 285983 1.1.1.149 0.00195 - 9,10-phenanthrenequinone +/-0.0004 286001 1.1.1.149 0.002 - 5alpha-pregnan-3alpha-ol-20-one pH 7.0, 25°C 655220 1.1.1.149 0.002 - 6-tert-butyl-2,3-epoxy-5-cyclohexen-1,4-dione at pH 7.4 and 37°C 740126 1.1.1.149 0.002 - NADPH +/-0.0001, ethyl acetoacetate 285999 1.1.1.149 0.002 0.003 17alpha-hydroxypregnenolone - 285999 1.1.1.149 0.00208 - 3-acetylpyridine adenine dinucleotide phosphate NADP analog 285983 1.1.1.149 0.0021 - NADPH mutant H222S, pH 7.5, 37°C 656526 1.1.1.149 0.0022 - 20alpha-hydroxyprogesterone at pH 7.4 and 37°C 740126 1.1.1.149 0.0022 - 5alpha-androstan-3beta,17beta-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.0023 - 20alpha-hydroxy-4-pregnen-3-one - 285981 1.1.1.149 0.0024 - n-hexyl acetoacetate - 285999 1.1.1.149 0.0025 - 5beta-Androstan-3beta,17beta-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.0026 - 2-amino-8-((2-fluoro-4-methoxybenzyl)thio)-1,9-dihydro-6H-purin-6-one pH 7.0, 25°C 655220 1.1.1.149 0.0026 - 5beta-Pregnan-3alpha,21-diol-20-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0027 - 5alpha-pregnan-20alpha-ol-3-one pH 7.0, 25°C 655220 1.1.1.149 0.0028 - 5beta-androstan-3beta-ol-17-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0029 - 17alpha-hydroxyprogesterone - 285998 1.1.1.149 0.0029 - 7alpha-hydroxy-dehydroepiandrosterone at pH 7.4 and 37°C 740126 1.1.1.149 0.003 - 17alpha-hydroxyprogesterone - 285999 1.1.1.149 0.003 0.012 17alpha-hydroxyprogesterone - 669908 1.1.1.149 0.0031 - 5-androstene-3beta,17beta-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.0033 - 11-deoxycorticosterone pH 7.4, 37°C 668428 1.1.1.149 0.0033 - 5alpha-pregnan-3alpha-ol-20-one pH 7.0, 25°C 655220 1.1.1.149 0.0033 - 5beta-Pregnan-3alpha-ol-20-one pH 7.0, 25°C 655220 1.1.1.149 0.0034 - 17alpha-hydroxypregnenolone - 285998 1.1.1.149 0.0034 - 5alpha-dihydrotestosterone at pH 7.4 and 37°C 740126 1.1.1.149 0.004 - n-butyl acetoacetate - 285999 1.1.1.149 0.0041 - 5alpha-Androstan-3alpha-ol-17-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0041 - progesterone mutant T307V, pH 7.5, 37°C 656526 1.1.1.149 0.0042 - 5alpha-androstan-3alpha,17beta-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.0042 - dehydroepiandrosterone 3-sulfate at pH 7.4 and 37°C 740126 1.1.1.149 0.0042 - progesterone wild type, pH 7.5, 37°C 656526 1.1.1.149 0.0045 - 5beta-Pregnan-3alpha-ol-20-one pH 7.0, 25°C 655220 1.1.1.149 0.0045 - 5beta-Pregnan-3alpha-ol-20-one wild-type, pH 7.4, 25°C 711423 1.1.1.149 0.0046 - progesterone mutant D309L, pH 7.5, 37°C 656526 1.1.1.149 0.00475 - 20alpha-hydroxy-4-pregnen-3-one HSD1 285978 1.1.1.149 0.005 0.008 2,3-heptanedione - 285999 1.1.1.149 0.00516 - 20alpha-hydroxy-4-pregnen-3-one HSD2 285978 1.1.1.149 0.0053 - 4-hydroxy-2-nonenal at pH 7.4 and 37°C 740126 1.1.1.149 0.00538 - progesterone spectrophotometric pH 6.8 285983 1.1.1.149 0.0055 - trans-2-nonenal at pH 7.4 and 37°C 740126 1.1.1.149 0.0056 - progesterone mutant Y305F, pH 7.5, 37°C 656526 1.1.1.149 0.0057 - 5beta-pregnan-20alpha-ol-3-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0058 - 17alpha-hydroxyprogesterone pH 7.4, 37°C 668428 1.1.1.149 0.0058 0.0059 20alpha-hydroxyprogesterone 37°C, 10 min 286000 1.1.1.149 0.0059 - 4-oxo-2-hexenal at pH 7.4 and 37°C 740126 1.1.1.149 0.0059 - 5alpha-pregnan-20alpha-ol-3-one at pH 7.4 and 37°C 740126 1.1.1.149 0.006 - 1-phenyl-1,2-propanedione - 285999 1.1.1.149 0.006 - 5alpha-Androstan-3beta-ol-17-one at pH 7.4 and 37°C 740126 1.1.1.149 0.006 - dehydroepiandrosterone at pH 7.4 and 37°C 740126 1.1.1.149 0.0062 - 5alpha-Pregnan-3alpha,21-diol-20-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0062 - 5beta-androstan-17beta-ol-3-one at pH 7.4 and 37°C 740126 1.1.1.149 0.0062 - 5beta-Pregnan-3alpha-ol-20-one mutant L308V, pH 7.4, 25°C 711423 1.1.1.149 0.0066 - progesterone NADPH, cell homogenate 285997 1.1.1.149 0.0067 - Estrone 3-sulfate at pH 7.4 and 37°C 740126 1.1.1.149 0.007 - (S)-tetralol wild-type, pH 7.4, 25°C 711423 1.1.1.149 0.0074 - trans-2-hexenal at pH 7.4 and 37°C 740126 1.1.1.149 0.0075 - 20alpha-hydroxyprogesterone - 286003 1.1.1.149 0.0076 - hexanal at pH 7.4 and 37°C 740126 1.1.1.149 0.0078 - 5beta-pregnan-3alpha,20alpha-diol at pH 7.4 and 37°C 740126 1.1.1.149 0.008 - benzyl acetoacetate - 285999 1.1.1.149 0.008 - NADP+ +/-0.0011 286001 1.1.1.149 0.008 - NADP+ 20-oxo and 20alpha-hydroxy-steroids, pH 7.0 285998 1.1.1.149 0.0081 - NADH cortisone 0.05 mM 285979 1.1.1.149 0.0089 - all-trans-retinal at pH 7.4 and 37°C 740126 1.1.1.149 0.009 - 20alpha-hydroxyprogesterone +/-0.0001 286001 1.1.1.149 0.0094 - progesterone mutant E127D, pH 7.5, 37°C 656526 1.1.1.149 0.0095 0.0096 NADP+ 37°C, 7.5 min 286000 1.1.1.149 0.01 0.016 2,3-Hexanedione - 285999 1.1.1.149 0.011 - 5alpha-pregnan-20alpha-ol-3-one at pH 7.4 and 37°C 740126 1.1.1.149 0.011 - progesterone +/-0.0017 286001 1.1.1.149 0.012 - 3alpha-hydroxy-5alpha-pregnan-20-one +/-0.0035 286001 1.1.1.149 0.012 - NADP+ +/-0.001, (S)-ethyl 3-hydroxybutyrate 285999 1.1.1.149 0.012 0.015 ethyl 2-methylacetoacetate - 285999 1.1.1.149 0.013 - 17alpha,20alpha-dihydroxypregn-4-en-3-one - 285998 1.1.1.149 0.013 - 20alpha-hydroxy-5alpha-pregnan-3-one +/-0.0035 286001 1.1.1.149 0.013 - testosterone at pH 7.4 and 37°C 740126 1.1.1.149 0.013 0.014 17alpha,20alpha-dihydroxyprogesterone - 285999 1.1.1.149 0.014 - acrolein at pH 7.4 and 37°C 740126 1.1.1.149 0.014 - NADPH +/-0.004 286001 1.1.1.149 0.015 0.018 2,3-Butanedione - 285999 1.1.1.149 0.0154 - 17alpha-hydroxyprogesterone +/-0.0029 286001 1.1.1.149 0.016 - (S)-tetralol mutant L308V, pH 7.4, 25°C 711423 1.1.1.149 0.016 - 4-pregnen-20alpha-ol-3-one pH 7.0, 25°C 655220 1.1.1.149 0.018 - 5alpha-dihydrotestosterone at pH 7.4 and 37°C 740126 1.1.1.149 0.018 - beta-ionol at pH 7.4 and 37°C 740126 1.1.1.149 0.018 - menadione at pH 7.4 and 37°C 740126 1.1.1.149 0.018 0.021 2,3-Pentanedione - 285999 1.1.1.149 0.019 - diacetyl at pH 7.4 and 37°C 740126 1.1.1.149 0.019 - NADPH mutant H222S, pH 7.5, 37°C 656526 1.1.1.149 0.02 - 17alpha,21-dihydroxy-5beta-pregnane-3,11,20-trione - 285998 1.1.1.149 0.02 0.022 acetoacetyl-CoA - 285999 1.1.1.149 0.02 0.032 ethyl acetoacetate - 285999 1.1.1.149 0.022 - 17alpha,21-dihydroxy-5beta-pregnan-3,20-dione - 285998 1.1.1.149 0.022 - cortisone NADH, pH 6.3 285979 1.1.1.149 0.022 - NADPH 10 mM cysteine, at 4°C until activity decreases to half of the initial value 285988 1.1.1.149 0.02407 - cortisol pH 5.0, temperature not specified in the publication 763262 1.1.1.149 0.025 - ethyl pyruvate - 285999 1.1.1.149 0.025 - NADP+ 0.01-0.1 mM, 6 min 285984 1.1.1.149 0.0256 - progesterone mutant H222S, pH 7.5, 37°C 656526 1.1.1.149 0.0262 - 17alpha-hydroxyprogesterone isozyme HSD-I 285990 1.1.1.149 0.027 - ethyl 4-chloroacetoacetate - 285999 1.1.1.149 0.0286 - progesterone mutant H222I, pH 7.5, 37°C 656526 1.1.1.149 0.032 - cortisol NADH, pH 6.3 285979 1.1.1.149 0.034 - befunolol at pH 7.4 and 37°C 740126 1.1.1.149 0.039 0.045 isatin - 285999 1.1.1.149 0.041 - 20alpha-dihydrocortisol NAD+, pH 7.9 285979 1.1.1.149 0.049 0.051 Pyridine-3-aldehyde - 285999 1.1.1.149 0.052 - NADPH 10 mM cysteine 285988 1.1.1.149 0.053 - alpha-tetralone at pH 7.4 and 37°C 740126 1.1.1.149 0.0588 - nicotinamide hypoxanthine dinucleotide phosphate NADP analog 285983 1.1.1.149 0.061 - pyridine 3-aldehyde at pH 7.4 and 37°C 740126 1.1.1.149 0.064 - isatin - 285998 1.1.1.149 0.067 - ethyl acetoacetate - 285998 1.1.1.149 0.068 - isocaproaldehyde at pH 7.4 and 37°C 740126 1.1.1.149 0.069 - dihydrotestosterone wild type, pH 7.5, 37°C 656526 1.1.1.149 0.073 - 17-hydroxyprogesterone - 285991 1.1.1.149 0.073 - 20alpha-hydroxy-5beta-pregnan-3-one - 285998 1.1.1.149 0.073 - 5beta-pregnan-20alpha-ol-3-one - 285999 1.1.1.149 0.078 - Camphorquinone - 285998 1.1.1.149 0.081 - Pyridine-3-aldehyde - 285998 1.1.1.149 0.088 - methylglyoxal at pH 7.4 and 37°C 740126 1.1.1.149 0.092 0.129 1,2-Cyclohexanedione - 285999 1.1.1.149 0.098 - 20alpha-hydroxyprogesterone mutant H222I, pH 7.5, 37°C 656526 1.1.1.149 0.111 - ketotifen at pH 7.4 and 37°C 740126 1.1.1.149 0.112 - (S)-1-tetralol at pH 7.4 and 37°C 740126 1.1.1.149 0.117 0.123 methyl acetoacetate - 285999 1.1.1.149 0.118 - 17alpha-hydroxyprogesterone isozyme HSD-II 285990 1.1.1.149 0.12 - 20alpha-hydroxyprogesterone A, B and C loop changed, total converted enzyme 286003 1.1.1.149 0.123 - methyl acetoacetate - 285999 1.1.1.149 0.125 - 1,2-Cyclohexanedione - 285998 1.1.1.149 0.129 - 1,2-Cyclohexanedione - 285999 1.1.1.149 0.13 - 20alpha-hydroxyprogesterone A and C loop changed, bifunctional enzyme 286003 1.1.1.149 0.131 - (S)-1-indanol at pH 7.4 and 37°C 740126 1.1.1.149 0.131 0.243 2,4-Pentanedione - 285999 1.1.1.149 0.1429 - nicotinamide 1,N6-ethenoadenine dinucleotide phosphate NADP analog 285983 1.1.1.149 0.151 - dihydrotestosterone mutant R304L, pH 7.5, 37°C 656526 1.1.1.149 0.152 - 4-chromanol at pH 7.4 and 37°C 740126 1.1.1.149 0.162 - acetoin at pH 7.4 and 37°C 740126 1.1.1.149 0.1774 - 20alpha-hydroxyprogesterone mutant H222S, pH 7.5, 37°C 656526 1.1.1.149 0.18 - geraniol at pH 7.4 and 37°C 740126 1.1.1.149 0.186 0.272 acetoin - 285999 1.1.1.149 0.2 - progesterone - 285977 1.1.1.149 0.21 - 2,4-Pentanedione - 285998 1.1.1.149 0.221 0.254 4-nitrobenzaldehyde - 285999 1.1.1.149 0.222 0.447 ethyl 3-oxovalerate - 285999 1.1.1.149 0.23 0.57 3,4-hexanedione - 285999 1.1.1.149 0.243 - 2,4-Pentanedione - 285999 1.1.1.149 0.26 - trans-benzene dihydrodiol at pH 7.4 and 37°C 740126 1.1.1.149 0.272 - acetoin - 285999 1.1.1.149 0.2833 - progesterone mutant R304L, pH 7.5, 37°C 656526 1.1.1.149 0.3 - 20alpha-hydroxyprogesterone 0.015-0.1 mM, 6 min 285984 1.1.1.149 0.3 0.464 D-glyceraldehyde - 285999 1.1.1.149 0.303 0.654 ethyl benzoylacetate - 285999 1.1.1.149 0.34 - 4-nitrobenzaldehyde - 285998 1.1.1.149 0.356 0.548 methylglyoxal - 285999 1.1.1.149 0.3811 - 4-nitrobenzaldehyde +/-0.0605 286001 1.1.1.149 0.43 - Phenylglyoxal - 285998 1.1.1.149 0.447 - ethyl 3-oxovalerate - 285999 1.1.1.149 0.447 0.591 Phenylglyoxal - 285999 1.1.1.149 0.464 - D-glyceraldehyde - 285999 1.1.1.149 0.47 - (S)-tetralol mutant L308A, pH 7.4, 25°C 711423 1.1.1.149 0.526 - NAD+ 20alpha-dihydrocortisol 285979 1.1.1.149 0.534 0.587 D-Lactaldehyde - 285999 1.1.1.149 0.57 - 3,4-hexanedione - 285999 1.1.1.149 0.587 - D-Lactaldehyde - 285999 1.1.1.149 0.591 - Phenylglyoxal - 285999 1.1.1.149 0.654 - ethyl benzoylacetate - 285999 1.1.1.149 0.67 - D-glyceraldehyde - 285998 1.1.1.149 0.75 - NADH 10 mM cysteine 285988 1.1.1.149 0.75 - NADH freshly thawed enzyme 285988 1.1.1.149 0.852 1.3 DL-glyceraldehyde 3-phosphate - 285999 1.1.1.149 0.945 1.06 Pyridine-4-aldehyde - 285999 1.1.1.149 1 1.3 (S)-ethyl 3-hydroxybutyrate - 285999 1.1.1.149 1.06 - Pyridine-4-aldehyde - 285999 1.1.1.149 1.1 - NADH 10 mM cysteine, at 4°C until activity decreases to half of the initial value 285988 1.1.1.149 1.1 - NADH enzyme solution that has been left standing at 4°C until the activity has decreased to half of the initial value 285988 1.1.1.149 1.3 - DL-glyceraldehyde 3-phosphate - 285999 1.1.1.149 1.4 - 3-deoxyglucosone at pH 7.4 and 37°C 740126 1.1.1.149 1.6 - trans-benzene-1S,2S-dihydrodiol - 285999 1.1.1.149 2.02 2.9 cis-benzene-1,2-dihydrodiol racemic 285999 1.1.1.149 2.1 - benzene dihydrodiol - 285998 1.1.1.149 2.5 - 20alpha-hydroxyprogesterone - 285977 1.1.1.149 2.9 - cis-benzene-1,2-dihydrodiol racemic 285999 1.1.1.149 4.8 5 (S)-methyl 3-hydroxybutyrate - 285999 1.1.1.149 20 - ethyl acetoacetate +/-1 285999 1.1.1.149 34.8 66.3 2,3-Butanediol - 285999 1.1.1.149 50.9 79.7 trans-1S,2S-cyclohexanediol - 285999 1.1.1.149 66.3 - 2,3-Butanediol - 285999 1.1.1.149 79.7 - trans-1S,2S-cyclohexanediol - 285999 1.1.1.149 120 - D,L-glyceraldehyde - 286001 1.1.1.149 1870 - (S)-ethyl 3-hydroxybutyrate +/-60 285999