1.1.1.103 additional information - additional information kinetic analysis of Glu152 mutants, overview 687461 1.1.1.103 additional information - additional information kinetics 669546 1.1.1.103 0.00057 - L-threonine 25°C, pH 10, mutant enzyme G114A 762551 1.1.1.103 0.0026 - L-threonine 25°C, pH 10, wild-type enzyme 762551 1.1.1.103 0.00802 - n-butanol at pH 8.8 and 37°C 738860 1.1.1.103 0.0088 - L-threonine 25°C, pH 10, mutant enzyme I113A 762551 1.1.1.103 0.0099 - NAD+ pH 7.5, 65°C, recombinant wild-type enzyme 687461, 688343 1.1.1.103 0.01 - NAD+ pH 7.5, 65°C 669546 1.1.1.103 0.011 - L-threonine 25°C, pH 10, mutant enzyme T178A 762551 1.1.1.103 0.01129 - L-threonine at pH 8.8 and 37°C 738860 1.1.1.103 0.0118 - L-threonine pH 7.5, 65°C, recombinant wild-type enzyme 688343 1.1.1.103 0.013 - L-threonine pH 7.5, 65°C 669546 1.1.1.103 0.014 - NAD+ 30°C, pH not specified in the publication, mutant enzyme S74A 762728 1.1.1.103 0.0155 - NAD+ 30°C, pH not specified in the publication, mutant enzyme D179N 762728 1.1.1.103 0.0164 - NAD+ - 285703 1.1.1.103 0.0196 - NAD+ 30°C, pH not specified in the publication, mutant enzyme S111A 762728 1.1.1.103 0.0243 - NAD+ 30°C, pH not specified in the publication, mutant enzyme D179A 762728 1.1.1.103 0.0257 - NAD+ 30°C, pH not specified in the publication, mutant enzyme T177A 762728 1.1.1.103 0.028 - NAD+ at 50°C 698673 1.1.1.103 0.043 - L-threonine 25°C, pH 10, mutant enzyme D180A 762551 1.1.1.103 0.047 - NAD+ - 285720 1.1.1.103 0.0507 - NAD+ 30°C, pH not specified in the publication, wild-type enzyme 762728 1.1.1.103 0.055 - NAD+ pH 7.0, 70°C, recombinant enzyme 668567 1.1.1.103 0.061 - L-serine 25°C, pH 10, wild-type enzyme 762551 1.1.1.103 0.0615 - NAD+ pH 7.5, 65°C, recombinant mutant E152A 687461 1.1.1.103 0.097 - NAD+ mutant enzyme T186N, at pH 10.0 and 30°C 738606 1.1.1.103 0.1 - NAD+ pH 10.0, 30°C, native enzyme 721272 1.1.1.103 0.1 - NAD+ pH 9.0, 37°C 729112 1.1.1.103 0.11 - NAD+ demetallized enzyme 285714 1.1.1.103 0.11 - NAD+ metal-ion free enzyme 285712 1.1.1.103 0.115 - NAD+ mutant enzyme L80G, at pH 10.0 and 30°C 738606 1.1.1.103 0.12 - NAD+ wild type enzyme, at pH 10.0 and 30°C 738606 1.1.1.103 0.14 - NAD+ at pH 7.5 285719 1.1.1.103 0.152 - NAD+ pH 7.5, 65°C, recombinant mutant E152T 687461 1.1.1.103 0.156 - NAD+ mutant enzyme G184A, at pH 10.0 and 30°C 738606 1.1.1.103 0.163 - NAD+ pH 7.5, 65°C, recombinant mutant E152C 687461 1.1.1.103 0.18 - L-serine 25°C, pH 10, mutant enzyme G114A 762551 1.1.1.103 0.18 - NAD+ enzyme activated with 0.07 mM Cd2+ 285712 1.1.1.103 0.185 - NAD+ pH 10.0, 70°C 722747 1.1.1.103 0.19 - NAD+ - 285701, 285716, 285718 1.1.1.103 0.216 - NAD+ pH 7.5, 65°C, recombinant mutant E152Q 687461 1.1.1.103 0.2225 - ethanol at pH 8.8 and 37°C 738860 1.1.1.103 0.24 - NAD+ - 285721 1.1.1.103 0.291 - NAD+ pH 7.5, 65°C, recombinant mutant R204A 688343 1.1.1.103 0.304 - NAD+ pH 7.5, 65°C, recombinant mutant E199A 688343 1.1.1.103 0.36 - (R)-(-)-1-amino-2-propanol 25°C, pH 10, mutant enzyme G114A 762551 1.1.1.103 0.399 - NAD+ pH 7.5, 65°C, recombinant mutant E152D 687461 1.1.1.103 0.428 - NAD+ 30°C, pH not specified in the publication, mutant enzyme Y136F 762728 1.1.1.103 0.6 - NAD+ enzyme activated with 0.25 mM Mn2+ 285712 1.1.1.103 0.6 - NAD+ enzyme saturated with 0.25 mM Mn2+ 285714 1.1.1.103 0.607 - NAD+ pH 7.5, 65°C, recombinant mutant E152S 687461 1.1.1.103 0.7 - L-threonine - 285703 1.1.1.103 0.75 - NAD+ C38D mutant 285702 1.1.1.103 0.98 - NAD+ - 285717 1.1.1.103 1 - NAD+ - 285713, 285715 1.1.1.103 1.1 - L-threonine demetallized enzyme 285714 1.1.1.103 1.1 - L-threonine metal-ion free enzyme 285712 1.1.1.103 1.43 - L-threonine - 285716, 285718 1.1.1.103 1.5 - L-threonine pH 7.0, 70°C, recombinant enzyme 668567 1.1.1.103 1.51 - L-threonine pH 7.5, 65°C, recombinant mutant E199A 688343 1.1.1.103 1.6 - L-serine 25°C, pH 10, mutant enzyme T178A 762551 1.1.1.103 1.6 - L-threonine at 50°C 698673 1.1.1.103 1.67 - NAD+ wild type enzyme, at pH 7.5 and 37°C 738933 1.1.1.103 1.8 - L-serine 25°C, pH 10, mutant enzyme I113A 762551 1.1.1.103 2.06 - NAD+ mutant enzyme R180K, at pH 7.5 and 37°C 738933 1.1.1.103 2.5 - L-threonine pH 7.5, 65°C, recombinant mutant R204A 688343 1.1.1.103 3.56 - L-threonine pH 9.0, 20°C 655857 1.1.1.103 3.81 - L-threonine pH 9.0, 10°C 655857 1.1.1.103 5.38 - L-threonine - 285701 1.1.1.103 5.5 - L-threonine - 285715 1.1.1.103 5.96 - L-threonine pH 9.0, 30°C 655857 1.1.1.103 6.1 - L-threonine - 285721 1.1.1.103 8.2 - L-threonine enzyme activated with 0.07 mM Cd2+ 285712 1.1.1.103 8.4 - L-threonine - 285717 1.1.1.103 8.7 - L-threonine - 285720 1.1.1.103 10.5 - L-threonine at pH 7.5 285719 1.1.1.103 11.2 - L-threonine pH 9.0, 40°C 655857 1.1.1.103 11.6 - L-threonine pH 10.0, 30°C, native enzyme 721272 1.1.1.103 13 - L-threonine - 285713 1.1.1.103 15.2 - L-threonine wild type enzyme, at pH 10.0 and 30°C 738606 1.1.1.103 16 - L-allothreonine - 285713 1.1.1.103 17.5 - L-threonine pH 10.0, 70°C 722747 1.1.1.103 18 - L-threonine pH 9.0, 37°C 729112 1.1.1.103 19.5 - L-threonine pH 9.0, 50°C 655857 1.1.1.103 23.7 - L-threonine 30°C, pH not specified in the publication, mutant enzyme T177A 762728 1.1.1.103 25.3 - L-threonine 30°C, pH not specified in the publication, wild-type enzyme 762728 1.1.1.103 40.6 - L-threonine 30°C, pH not specified in the publication, mutant enzyme Y136F 762728 1.1.1.103 72.4 - L-threonine wild type enzyme, at pH 7.5 and 37°C 738933 1.1.1.103 151.9 - L-threonine mutant enzyme M333E, at pH 7.5 and 37°C 738933 1.1.1.103 211 - L-threonine enzyme activated with 0.25 mM Mn2+ 285712 1.1.1.103 221 - L-threonine enzyme saturated with 0.25 mM Mn2+ 285714 1.1.1.103 246 - L-threonine 30°C, pH not specified in the publication, mutant enzyme S111A 762728