2.4.2.17 additional information - additional information steady-state kinetics 659965 2.4.2.17 0.015 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C 737208 2.4.2.17 0.015 - 5-phospho-alpha-D-ribose 1-diphosphate wild type long-form enzyme, at pH 8.5 and 25°C 758757 2.4.2.17 0.0184 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, wild-type enzyme 678174 2.4.2.17 0.028 - 5-phospho-alpha-D-ribose 1-diphosphate truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C 758757 2.4.2.17 0.049 - 5-phospho-alpha-D-ribose 1-diphosphate pH 8.5, 25°C 721673 2.4.2.17 0.067 - 5-phospho-alpha-D-ribose 1-diphosphate - 637661 2.4.2.17 0.07 - 5-phospho-alpha-D-ribose 1-diphosphate A270P mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.0712 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme D155A 678174 2.4.2.17 0.08 - 5-phospho-alpha-D-ribose 1-diphosphate N215K mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.08 - 5-phospho-alpha-D-ribose 1-diphosphate N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.08 - 5-phospho-alpha-D-ribose 1-diphosphate wild type protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.09 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K8A 678174 2.4.2.17 0.09 - 5-phospho-alpha-D-ribose 1-diphosphate L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.096 - 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.097 - ATP at pH 8.5 and 25°C 737208 2.4.2.17 0.097 - ATP wild type long-form enzyme, at pH 8.5 and 25°C 758757 2.4.2.17 0.111 - 1-(5-phospho-beta-D-ribosyl)-ATP at pH 8.5 and 5°C 777101 2.4.2.17 0.117 - ATP truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C 758757 2.4.2.17 0.13 - 5-phospho-alpha-D-ribose 1-diphosphate recombinant At-ATP-PRT1 637673 2.4.2.17 0.14 - 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.182 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C 758758 2.4.2.17 0.19 - ATP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.2 - ATP - 637661 2.4.2.17 0.22 - ATP N215K mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.22 - ATP wild type protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.23 - ATP at pH 8.5 and 5°C 777101 2.4.2.17 0.23 - ATP N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.24 - ATP A270P mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.263 - ATP pH 8.5, 25°C 721673 2.4.2.17 0.35 - ATP L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication 721765 2.4.2.17 0.36 - ADP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.37 - 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A, at pH 8.5 and 20°C 778695 2.4.2.17 0.39 - ATP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.4 - 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A/K57A, at pH 8.5 and 20°C 778695 2.4.2.17 0.41 - 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R32A, at pH 8.5 and 20°C 778695 2.4.2.17 0.44 - 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate,in the presence of Mn2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.44 - 5-phospho-alpha-D-ribose 1-diphosphate wild type enzyme, at pH 8.5 and 20°C 778695 2.4.2.17 0.47 - ATP at pH 8.0 and 24°C 758758 2.4.2.17 0.51 - ATP crude cell extracts 637673 2.4.2.17 0.57 - 5-phospho-alpha-D-ribose 1-diphosphate crude cell extracts 637673 2.4.2.17 0.6 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C 758806 2.4.2.17 0.6 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C 758806 2.4.2.17 0.6 - 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme C115S, at pH 8.5 and 20°C 778695 2.4.2.17 0.6 - 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.6 - 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mn2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.6 - ATP recombinant enzyme 637673 2.4.2.17 0.7 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C 758806 2.4.2.17 0.7 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C 758806 2.4.2.17 0.76 - ATP wild type enzyme, at pH 8.5 and 20°C 778695 2.4.2.17 0.8 - 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C 758806 2.4.2.17 0.83 - ATP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 0.9 - ATP mutant enzyme R56A, at pH 8.5 and 20°C 778695 2.4.2.17 0.901 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T162A 678174 2.4.2.17 0.94 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A 678174 2.4.2.17 1.05 - ATP 25°C, pH 8, mutant enzyme T162A 678174 2.4.2.17 1.07 - ATP 25°C, pH 8, mutant enzyme T159A 678174 2.4.2.17 1.2 - 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 1.2 - ATP at pH 8.5 and 30°C 758806 2.4.2.17 1.5 - ADP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C 776638 2.4.2.17 1.5 - ATP mutant enzyme R32A, at pH 8.5 and 20°C 778695 2.4.2.17 1.5 - ATP mutant enzyme R56A/K57A, at pH 8.5 and 20°C 778695 2.4.2.17 1.7 - ATP at pH 8.5 and 25°C 758806 2.4.2.17 1.8 - ATP at pH 8.5 and 20°C 758806 2.4.2.17 2 - ATP at pH 8.5 and 15°C 758806 2.4.2.17 2.1 - ATP at pH 8.5 and 10°C 758806 2.4.2.17 2.2 - ADP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C 776638 2.4.2.17 2.7 - ATP 25°C, pH 8, wild-type enzyme 678174 2.4.2.17 2.8 - ATP mutant enzyme C115S, at pH 8.5 and 20°C 778695 2.4.2.17 4.7 - ATP 25°C, pH 8, mutant enzyme D155A 678174 2.4.2.17 5 - ATP 25°C, pH 8, mutant enzyme K50A 678174 2.4.2.17 5.15 - 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T159A 678174 2.4.2.17 8.45 - ATP 25°C, pH 8, mutant enzyme K8A 678174 2.4.2.17 37 - 5-phospho-alpha-D-ribose 1-diphosphate crude cell extracts 637673 2.4.2.17 89 - ATP crude cell extracts 637673