2.1.1.67 additional information - additional information various thiophenols as substrates, Km 0.0008-0.0078 485518 2.1.1.67 0.00035 - 6-Mercaptopurine - 685254 2.1.1.67 0.00068 - 6-Mercaptopurine wild-type 685254 2.1.1.67 0.00091 - 6-Mercaptopurine mutant R226H 685254 2.1.1.67 0.0012 - 6-Mercaptopurine mutant R226E 685254 2.1.1.67 0.00143 - 6-Mercaptopurine mutant R152H 685254 2.1.1.67 0.00161 - 6-Mercaptopurine mutant R226A 685254 2.1.1.67 0.0024 - S-adenosyl-L-methionine cell lysate 485520 2.1.1.67 0.0027 - S-adenosyl-L-methionine - 485516, 485520 2.1.1.67 0.00273 - 6-Mercaptopurine mutant R152A 685254 2.1.1.67 0.0035 - tellurite pH 6.8, 37°C 776531 2.1.1.67 0.00463 - 6-Mercaptopurine mutant R152E 685254 2.1.1.67 0.0056 - S-adenosyl-L-methionine - 685254 2.1.1.67 0.00632 - 6-Mercaptopurine mutant R152A/R226A 685254 2.1.1.67 0.00637 - S-adenosyl-L-methionine isoenzyme II 485519 2.1.1.67 0.00665 - S-adenosyl-L-methionine Molt-F4 cell 657544 2.1.1.67 0.00722 - S-adenosyl-L-methionine isoenzyme I 485519 2.1.1.67 0.0106 - 6-Mercaptopurine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0109 - methanetellurol pH 6.8, 37°C 776531 2.1.1.67 0.0119 - S-adenosyl-L-methionine erythrocyte 657544 2.1.1.67 0.0127 - 6-thiodeoxyinosine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0137 - 6-thioguanine mutant L69V, cytosolic fraction of yeast cells is incubated with 500 microM of 6-TG and 0-400 microM of SAM 685039 2.1.1.67 0.0148 - S-adenosyl-L-methionine mutant R226E 685254 2.1.1.67 0.0152 - 6-thioguanine - 657885 2.1.1.67 0.0164 - S-adenosyl-L-methionine peripheral blood mononuclear cell 657544 2.1.1.67 0.0181 - 6-thioguanine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0185 - S-adenosyl-L-methionine wild-type 685254 2.1.1.67 0.0188 - 6-thioguanine 1.2fold increase in KM-value compared to wild-type variant 657885 2.1.1.67 0.0197 - S-adenosyl-L-methionine mutant R226A 685254 2.1.1.67 0.0199 - S-adenosyl-L-methionine mutant R226H 685254 2.1.1.67 0.0237 - S-adenosyl-L-methionine mutant R152H 685254 2.1.1.67 0.0254 - S-adenosyl-L-methionine mutant R152A 685254 2.1.1.67 0.0257 - 6-thioinosine 5'-monophosphate recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0261 - 6-thioguanosine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0271 - 6-thioguanosine 5-monophosphate recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0291 - 6-selenopurine - 485521 2.1.1.67 0.03435 - 6-thioguanine wild-type enzyme, cytosolic fraction of yeast cells is incubated with 500 microM of 6-TG and 0-400 microM of SAM 685039 2.1.1.67 0.0349 - 6-thioguanine - 657749 2.1.1.67 0.0445 - S-adenosyl-L-methionine mutant R152E 685254 2.1.1.67 0.0448 - 6-thioguanine 2.9fold increase in KM-value compared to wild-type variant 657885 2.1.1.67 0.0463 - S-adenosyl-L-methionine mutant R152A/R226A 685254 2.1.1.67 0.0518 - 6-selenopurine-riboside - 485521 2.1.1.67 0.0551 - 6-thioinosine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.0555 - 6-thioguanine the Km-value of the variant Ser125Leu is 1.6fold higher than the Km-value of the wild-type variant, the ratio of Vmax to Km-value is 27% of the wild-type ratio 657749 2.1.1.67 0.056 - 6-thioguanine mutant R215H, variant TPMT*8 689365 2.1.1.67 0.0562 - 6-thioguanine mutant L69V, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM 685039 2.1.1.67 0.0627 - 6-thioguanine wild-type enzyme, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM 685039 2.1.1.67 0.0635 - 6-thioguanine the Km-value of the variant Gly144Arg is 1.8fold higher than the Km-value of the wild-type variant, the ratio of Vmax to Km-value is 31% of the wild-type ratio 657749 2.1.1.67 0.072 - 6-thioguanine mutant C212R, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM 685039 2.1.1.67 0.0759 - 6-thioguanine the Km-value of the variant Glu28Val is 2.2fold higher than the Km-value of the wild-type variant, the ratio of Vmax to KM-value is 58% of the wild-type ratio 657749 2.1.1.67 0.076 - 6-thioguanine mutant K119T, variant TPMT*9 689365 2.1.1.67 0.07985 - 6-thioguanine mutant K119T, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM 685039 2.1.1.67 0.08 - 6-thioguanine wild-type, variant TPMT*1 689365 2.1.1.67 0.08305 - 6-thioguanine mutant Q179H, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM 685039 2.1.1.67 0.0961 - 8-hydroxy-6-mercaptopurine - 485521 2.1.1.67 0.098 - 6-thioguanine mutant A167G, variant TPMT*23 689365 2.1.1.67 0.125 - 6-thioguanine mutant G144R, variant TPMT*10 689365 2.1.1.67 0.127 - 6-thioguanine mutant S125L, variant TPMT*12 689365 2.1.1.67 0.13 - 6-thioguanine - 485527 2.1.1.67 0.1314 - 6-thiodeoxyguanosine recombinant enzyme, expressed in yeast 485522 2.1.1.67 0.138 - 6-hydroxy-8-mercaptopurine - 485521 2.1.1.67 0.139 - 6-selenoguanine-riboside - 485521 2.1.1.67 0.151 - 6-thioguanine mutant A80P, variant TPMT*2 689365 2.1.1.67 0.156 - 6-Mercaptopurine - 485527 2.1.1.67 0.156 - 6-thioguanine mutant Y240C, variant TPMT*3C 689365 2.1.1.67 0.159 - 9-(n-propyl)-6-thioguanine - 485521 2.1.1.67 0.1799 - 6-thioguanine the Km-value of the variant Gly144Arg is 5.1fold higher than the Km-value of the wild-type variant, the ratio of Vmax to KM-value is 10.4% of the wild-type ratio 657749 2.1.1.67 0.186 - 6-thioguanine mutant K122T, variant TPMT*19 689365 2.1.1.67 0.2 - 6-Thiopurine - 485515 2.1.1.67 0.224 - 6-Mercaptopurine isoenzyme II 485519 2.1.1.67 0.231 - 7-methyl-6-mercaptourine - 485521 2.1.1.67 0.29 - 6-Mercaptopurine cell lysate 485520 2.1.1.67 0.292 - 9-(n-butyl)6-mercaptopurine - 485521 2.1.1.67 0.3 - 6-Mercaptopurine - 485516, 485520 2.1.1.67 0.307 - 6-Mercaptopurine isoenzyme I 485519 2.1.1.67 0.32 - 6-Mercaptopurine - 485520 2.1.1.67 0.353 - 6-thioguanine mutant K238E, variant TPMT*20 689365 2.1.1.67 0.372 - 9-ethyl-6-mercaptopurine - 485521 2.1.1.67 0.38 - 6-Mercaptopurine kidney enzyme 485525 2.1.1.67 0.383 - 6-Mercaptopurine - 485521 2.1.1.67 0.485 - 6-thioguanine mutant Y180F, variant TPMT*6 689365 2.1.1.67 0.54 - 6-Mercaptopurine erythrocyte 657544 2.1.1.67 0.55 - 6-thioguanine - 485516 2.1.1.67 0.55 - 6-thioguanine mutant E28V, variant TPMT*13 689365 2.1.1.67 0.557 - 6-thioguanine - 485521 2.1.1.67 0.58 - 6-Mercaptopurine - 485520 2.1.1.67 0.58 - 6-Mercaptopurine liver enzyme 485525 2.1.1.67 0.6 - 6-thioguanine mutant R163H, variant TPMT*16 689365 2.1.1.67 0.65 - 6-Mercaptopurine Molt-F4 cell 657544 2.1.1.67 0.761 - 6-thioguanine-riboside - 485521 2.1.1.67 0.85 - 6-Mercaptopurine peripheral blood mononuclear cell 657544 2.1.1.67 0.89 - 6-mercaptopurine-riboside-5'-triphosphate - 485521 2.1.1.67 0.99 - 6-Mercaptopurine recombinant enzyme, baculovirus expressed 485525 2.1.1.67 1.027 - 6-thioguanine mutant H227Q, variant TPMT*7 689365 2.1.1.67 1.04 - 6-thioguanine-riboside-5'-monophosphate - 485521 2.1.1.67 1.094 - 6-thioguanine mutant G36S, variant TPMT*24 689365 2.1.1.67 1.17 - 6-mercaptopurine-riboside - 485521 2.1.1.67 1.267 - 6-thioguanine mutant Q42E, variant TPMT*17 689365 2.1.1.67 1.27 - 6-mercaptopurine-riboside-5'-monophosphate - 485521 2.1.1.67 1.45 - 6-thioguanine mutant C132Y, variant TPMT*11 689365 2.1.1.67 1.7 - 2-Thiouracil - 485515 2.1.1.67 2 - 2-Thiouracil - 485516 2.1.1.67 168 - 2-mercaptoethanol - 485516