3.1.3.72 Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity 15 3.1.3.72 Ca2+ at concentrations equimolar to the substrate: 313% activation, but inhibitory at higher concentrations 15 3.1.3.72 Cu2+ - 19 3.1.3.72 CuSO4 moderately inhibits phytase activity 263 3.1.3.72 EDTA - 21 3.1.3.72 EDTA at 1 mM inhibits the enzyme activity up to 90% and completely at 5 mM concentration 21 3.1.3.72 EDTA 0.5 mM: 35% inhibition, 5 mM: 94% inhibition 21 3.1.3.72 EDTA 5 mM, complete inhibition due to a dependence on Ca2+ 21 3.1.3.72 Fe2+ - 25 3.1.3.72 Mg2+ - 6