3.4.22.33	Ca2+	noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5	15	
3.4.22.33	Cu2+	12% residual activity at 0.16 mM	19	
3.4.22.33	Cu2+	inhibits casein degradation, complete inhibition	19	
3.4.22.33	Cu2+	reduces activity	19	
3.4.22.33	Cu2+	competitive inhibition at pH 3.5 with 0.6 mM Cu2+, which changes to an uncompetitive inhibition at pH 4.5 and pH 7.5	19	
3.4.22.33	cystatin	-	1600	
3.4.22.33	cystatin	an extended AE-rich N-terminal trunk in secreted pineapple cystatin enhances inhibition of fruit bromelain and is posttranslationally removed during ripening, The AE-rich N-terminal trunk is required to inhibit fruit bromelain (above 95%), whereas its removal decreases inhibition to 20%. Recombinant cystatin containing the complete AE-rich N-terminal trunk and recombinant medium pineapple cystatin effectively inhibit bromelain compared to recombinant core pineapple cystatin	1600	
3.4.22.33	Fe3+	reduces activity	70	
3.4.22.33	Hg2+	14% residual activity at 0.16 mM	33	
3.4.22.33	Hg2+	inhibits casein degradation, complete inhibition	33	
3.4.22.33	Hg2+	-	33	
3.4.22.33	Hg2+	uncompetitive inhibition at pH 3.5 and pH 4.5 with 0.6 mM Hg2+	33	
3.4.22.33	iodoacetic acid	5% residual activity at 2 mM	213	
3.4.22.33	Mercurials	cysteine restores activity	5258	
3.4.22.33	Mercurials	cysteine restores activity; mercaptoethanol restores activity	5258	
3.4.22.33	additional information	scarcely inhibited by chicken chystatin	2	
3.4.22.33	additional information	enzyme is inhibited by cystatin	2	
3.4.22.33	additional information	not inhibited by pepstatin	2	
3.4.22.33	additional information	no effect on activity by EDTA	2	
3.4.22.33	additional information	stability of bromelain in the presence of EDTA and sodium benzoate; stability of bromelain in the presence of EDTA and sodium benzoate	2	
3.4.22.33	p-chloromercuribenzoate	15% residual activity at 2 mM	43	
3.4.22.33	PCMB	activity restored by cysteine	78	
3.4.22.33	Phenylmethylsulfonylfluoride	84% residual activity at 2 mM	1148	
3.4.22.33	PMSF	-	248	
3.4.22.33	Potato cysteine proteinase inhibitor	-	46560	
3.4.22.33	Sodium benzoate	strong inhibition at 0.5-1.0%	15963	
3.4.22.33	sodium metabisulphite	-	239858	
3.4.22.33	sulfhydryl reagents	-	978	
3.4.22.33	Urea	at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate	116