1.4.1.14 (2,5-dioxoimidazolidin-4-yl)urea activity of NADH-GOGAT I significantly decreases in the presence of 10 mM allantoine 143704 1.4.1.14 (2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid - 30781 1.4.1.14 (2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid potent irreversible inhibitor 30781 1.4.1.14 2-hydroxyglutarate D- and L-isomer, most potent competitive inhibitor 6427 1.4.1.14 2-oxoglutarate - 34 1.4.1.14 3-acetylpyridine adenine dinucleotide competitive inhibitor with respect to NADH 904 1.4.1.14 6-diazo-5-oxo-L-norleucine 0.1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition 839 1.4.1.14 6-diazo-5-oxo-L-norleucine 76% inhibition at 5 mM 839 1.4.1.14 6-diazo-5-oxo-L-norleucine - 839 1.4.1.14 acridine inhibitor of flavoenzymes, 65% inhibition 21809 1.4.1.14 ADP 10 mM, 84% inhibition 13 1.4.1.14 ADP 20 mM, about 75% inhibition 13 1.4.1.14 alanine slight inhibition 654 1.4.1.14 allopurinol activity of NADH-GOGAT I significantly decreases in the presence of 2 mM allopurinol 801 1.4.1.14 AMP 10 mM, 61% inhibition 30 1.4.1.14 AMP 20 mM, about 75% inhibition 30 1.4.1.14 arginine 20 mM, complete inhibition 532 1.4.1.14 asparagine weak inhibition, competitive with 2-oxoglutarate 2264 1.4.1.14 asparagine slight inhibition 2264 1.4.1.14 asparagine activity of NADH-GOGAT I significantly decreases in the presence of 10 mM asparagine 2264 1.4.1.14 aspartate weak inhibition, competitive with 2-oxoglutarate 724 1.4.1.14 aspartate 10 mM, 60% inhibition 724 1.4.1.14 Atebrin - 2721 1.4.1.14 ATP 10 mM, 37% inhibition 4 1.4.1.14 ATP 5 mM, 75% inhibition 4 1.4.1.14 azaserine completely inhibited by 4 mM 1861 1.4.1.14 azaserine glutamine antagonist, completely inhibited by 10 mM 1861 1.4.1.14 azaserine completely inhibited by 0.4 mM 1861 1.4.1.14 azaserine - 1861 1.4.1.14 azaserine complete inhibition 1861 1.4.1.14 azaserine completely inhibited by 1 mM, glutamine analog 1861 1.4.1.14 azaserine 75% inhibition at 5 mM 1861 1.4.1.14 bathophenanthroline 50% inhibition 2722 1.4.1.14 Cd2+ 0.5 mM slightly decreases GOGAT activity in the absence of 2-oxoglutarate 52 1.4.1.14 citrate inhibited by high concentrations, 40 mM 131 1.4.1.14 Co2+ slight inhibition 23 1.4.1.14 Cu2+ Cu2+ and Fe2+ are most inhibitory metal ions 19 1.4.1.14 D-2-hydroxyglutarate - 5143 1.4.1.14 D-glutamate competitive inhibitor 344 1.4.1.14 D-glutamine - 2138 1.4.1.14 D-glutamine slight inhibition 2138 1.4.1.14 DL-ethionine sulfone - 32629 1.4.1.14 DL-ethionine sulfone potent competitive inhibitor 32629 1.4.1.14 DL-homocysteic acid - 8098 1.4.1.14 Fe2+ Cu2+ and Fe2+ are most inhibitory metal ions 25 1.4.1.14 glutamine activity of NADH-GOGAT I significantly decreases in the presence of 10 mM glutamine 755 1.4.1.14 Glutarate most potent competitive inhibitor 545 1.4.1.14 glycine slight inhibition 72 1.4.1.14 glycine 48% inhibition at 10 mM, complete inhibition at 20 mM 72 1.4.1.14 L-2-Amino-4-oxo-5-chloropentanoate 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition 20323 1.4.1.14 L-2-hydroxyglutarate - 5986 1.4.1.14 L-Albizziin - 12576 1.4.1.14 L-Albizziin less inhibitory than L-homocysteine sulfonamide, methionine sulfone and ethionine sulfone 12576 1.4.1.14 L-glutamate competitive with 2-oxoglutarate 41 1.4.1.14 L-glutamate - 41 1.4.1.14 L-glutamate 4 mM, 20% inhibition, product inhibition 41 1.4.1.14 L-glutamate 10 mM, 24% inhibition 41 1.4.1.14 L-glutamate competitive with 2-oxoglutarate; glutamate binds 1.7times more tightly in the presence of NAD+ 41 1.4.1.14 L-histidine 25 mM, 20-30% inhibition 349 1.4.1.14 L-homocysteine sulfonamide very potent transition state inhibitor, competitive to L-glutamine 32667 1.4.1.14 L-methionine D,L-sulfoximine - 143705 1.4.1.14 L-methionine sulfone markedly inhibits 3081 1.4.1.14 L-methionine sulfone very potent competitive inhibitor 3081 1.4.1.14 L-methionine-SR-sulfoxide - 16102 1.4.1.14 L-methionine-SR-sulfoxide slight inhibition 16102 1.4.1.14 leucine 10 mM, 52% inhibition 641 1.4.1.14 malate inhibited by high concentrations, 40 mM 283 1.4.1.14 malate 20 mM, 30% inhibition 283 1.4.1.14 methionine 25 mM L-methionine, 20-30% inhibition 692 1.4.1.14 methionine 10 mM, 47% inhibition 692 1.4.1.14 methionine 10 mM, 72% inhibition 692 1.4.1.14 Mg2+ slight inhibition 6 1.4.1.14 additional information not inhibited by 2,2’-dipyridyl or N-ethylmaleimide 2 1.4.1.14 additional information not inhibited by Mn2+ and Ca2+ 2 1.4.1.14 additional information not inhibited by alanine, glycine, serine, lysine, proline, cysteine, valine, leucine, isoleucine, threonine, phenylalanine, histidine, tryptophan, asparagine, arginine, UTP, ITP, GTP 2 1.4.1.14 additional information cumulative inhibition by combinations of amino acids and nucleotides, cumulative feedback inhibition 2 1.4.1.14 additional information not inhibited by vitamin B6-enzyme inhibitors 2 1.4.1.14 additional information not inhibited by 1 mM cyanide or cyanate 2 1.4.1.14 additional information not inhibited by the 20 common amino acids at a concentration of 20 mM 2 1.4.1.14 additional information enzyme activity declines significantly between 42-68% and 9-42% relative water content in leaves 2 1.4.1.14 additional information the presence of asparagine, glutamine, xanthine, allantoin, allopurinol in the nutrient solution does not affect the expression of NADH-GOGAT-II in the nodules 2 1.4.1.14 NAD+ competitive with NADH 7 1.4.1.14 NAD+ - 7 1.4.1.14 NAD+ 1 mM, 27% inhibition 7 1.4.1.14 NADH substrate inhibition at high concentrations 8 1.4.1.14 NADP+ 1 mM, 48% inhibition 10 1.4.1.14 NADPH 0.2 mM, 50% inhibition 5 1.4.1.14 O-diazoacetyl-L-serine 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition 30291 1.4.1.14 oxaloacetate weak inhibition, competitive with 2-oxoglutarate 57 1.4.1.14 p-hydroxymercuribenzoate complete inhibition at 0.1-1 mM, at 0.0005 mM 33% inhibition or 75% inhibition after preincubation in presence of glutamine and 2-oxoglutarate, substrates induce a conformation, which makes essential sulfhydryl groups more accessible to reagent 98 1.4.1.14 phenylalanine 20 mM, complete inhibition 429 1.4.1.14 proline - 1625 1.4.1.14 pyruvate slight inhibition, analogue of 2-oxoglutarate 31 1.4.1.14 pyruvate 20 mM, 20% inhibition 31 1.4.1.14 serine 25 mM L-serine, 20-30% inhibition 930 1.4.1.14 serine 10 mM, 73% inhibition 930 1.4.1.14 Sodium dithionite strong inhibitory effect 3230 1.4.1.14 tryptophan 25 mM L-tryptophan, 20-30% inhibition 888 1.4.1.14 tryptophan 29% inhibition at 10 mM, complete inhibition at 20 mM 888 1.4.1.14 valine - 1210 1.4.1.14 xanthine activity of NADH-GOGAT I significantly decreases in the presence of 10 mM xanthine 234 1.4.1.14 Zn2+ slight inhibition 14