1.1.1.284 1,10-phenanthroline 1 mM, 30% inhibition 62 1.1.1.284 12-oxododecanoic acid substrate inhibition 16592 1.1.1.284 12-oxododecanoic acid linear competitive 16592 1.1.1.284 2,2'-dipyridyl 1 mM, 20% inhibition 375 1.1.1.284 2-mercaptoethanol inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM 63 1.1.1.284 3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol CBR1 inhibitor, does not inhibit NADH-dependent S-nitrosoglutathione reduction 155196 1.1.1.284 3-(5-(4-(1H-imidazol-1-yl) phenyl)-1-(4-carbamoyl-2-methylphenyl)-1H-pyrrol-2-yl) propionic acid N6022, a specific and potent GSNO-R inhibitor. N6022 inhibits the GSNO-R-mediated metabolism of GSNO and formaldehyde in the heart 259773 1.1.1.284 3-(5-(4-(1H-imidazol-1-yl) phenyl)-1-(4-carbamoyl-2-methylphenyl)-1H-pyrrol-2-yl) propionic acid N6022, a commercial potent and specific inhibitor of GSNOR 259773 1.1.1.284 3-[1-(4-acetylphenyl)-5-phenyl-1H-pyrrol-2-yl]propanoic acid exhibits noncompetitive or uncompetitive inhibition against varied concentrations of S-nitrosoglutathione or NADH 74212 1.1.1.284 3-[1-(4-acetylphenyl)-5-phenyl-1H-pyrrol-2-yl]propanoic acid 3-[1-(4-acetylphenyl)-5-phenyl-1H-pyrrol-2-yl]propanoic acid and 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid are more effective than 5-chloro-3-[2-[(4-ethoxyphenyl)(ethyl)amino]-2-oxoethyl]-1H-indole-2-carboxylic acid in inhibiting GSNOR inside the cells, dependence of S-nitrosothiols accumulation on the concentration of compound 74212 1.1.1.284 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid exhibits noncompetitive or uncompetitive inhibition against varied concentrations of S-nitrosoglutathione or NADH 74214 1.1.1.284 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid 3-[1-(4-acetylphenyl)-5-phenyl-1H-pyrrol-2-yl]propanoic acid and 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid are more effective than 5-chloro-3-[2-[(4-ethoxyphenyl)(ethyl)amino]-2-oxoethyl]-1H-indole-2-carboxylic acid in inhibiting GSNOR inside the cells, dependence of S-nitrosothiols accumulation on the concentration of compound. 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid increases the nitrosylation of cellular proteins in RAW264.7 cells in a time-dependent manner 74214 1.1.1.284 4-Methylpyrazole low sensitivity towards the potent inhibitor of alcohol dehydrogenase 1 enzymes 2464 1.1.1.284 5-chloro-3-(2-[4-ethoxyphenyl) (ethyl) amino]-2-oxoethyl)-1H-indole-2-carboxylic acid C2 259774 1.1.1.284 5-chloro-3-[2-[(4-ethoxyphenyl)(ethyl)amino]-2-oxoethyl]-1H-indole-2-carboxylic acid exhibits noncompetitive or uncompetitive inhibition against varied concentrations of S-nitrosoglutathione or NADH 74213 1.1.1.284 5-chloro-3-[2-[(4-ethoxyphenyl)(ethyl)amino]-2-oxoethyl]-1H-indole-2-carboxylic acid 3-[1-(4-acetylphenyl)-5-phenyl-1H-pyrrol-2-yl]propanoic acid and 4-([2-[(2-cyanobenzyl)sulfanyl]-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl]methyl)benzoic acid are more effective than 5-chloro-3-[2-[(4-ethoxyphenyl)(ethyl)amino]-2-oxoethyl]-1H-indole-2-carboxylic acid in inhibiting GSNOR inside the cells, dependence of S-nitrosothiols accumulation on the concentration of compound 74213 1.1.1.284 ADP - 13 1.1.1.284 ADP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione 13 1.1.1.284 ADP-ribose - 402 1.1.1.284 ADP-ribose competitive with respect to beta-NAD+ and non-competitive with glutathione 402 1.1.1.284 Ag+ - 75 1.1.1.284 AgNO3 NADH protects more effectively than NAD+ 360 1.1.1.284 Alpha-NAD+ - 5516 1.1.1.284 Alpha-NAD+ competitive with respect to beta-NAD+ and non-competitive with glutathione 5516 1.1.1.284 AMP - 30 1.1.1.284 AMP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione 30 1.1.1.284 ascorbic acid inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM 387 1.1.1.284 ATP - 4 1.1.1.284 ATP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione 4 1.1.1.284 Cd2+ - 52 1.1.1.284 Cd2+ 1 mM, complete inhibition 52 1.1.1.284 Cd2+ in pea leaves treated with 0.05 mM cadmium, GSNOR expression and activity are decreased by about 30% 52 1.1.1.284 citrate 1 mM, 24% inhibition 131 1.1.1.284 CN- 1 mM, 42% inhibition 159 1.1.1.284 CN- - 159 1.1.1.284 CN- 1 mM, 48% inhibition 159 1.1.1.284 Cu2+ - 19 1.1.1.284 Cu2+ 1 mM, complete inhibition 19 1.1.1.284 Cu2+ 1 mM, 76% inhibition 19 1.1.1.284 decanedioic acid sebacic acid 20233 1.1.1.284 Decanoic acid capric acid 2551 1.1.1.284 Decanoic acid - 2551 1.1.1.284 Decanoic acid noncompetitive inhibitor 2551 1.1.1.284 dithiothreitol inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM 45 1.1.1.284 dodecanedioic acid most efficient inhibitor 15745 1.1.1.284 dodecanoic acid - 2019 1.1.1.284 dodecanoic acid inhibits ADH3 irrespective of substrate 2019 1.1.1.284 dodecanoic acid lauric acid, most efficient inhibitor, competitively inhibits 2019 1.1.1.284 dodecanoic acid noncompetitive inhibition 2019 1.1.1.284 dodecanoic acid noncompetitive 2019 1.1.1.284 dodecanoic acid noncompetitive inhibitor 2019 1.1.1.284 EDTA complete inactivation 21 1.1.1.284 EDTA 1 mM, 12% inhibition 21 1.1.1.284 folic acid - 2840 1.1.1.284 formaldehyde - 130 1.1.1.284 glutathione - 44 1.1.1.284 glutathione noncompetitive inhibitor, inhibits GSNO reduction 44 1.1.1.284 GSH - 142 1.1.1.284 GSSG - 330 1.1.1.284 Hg2+ - 33 1.1.1.284 Hg2+ 1 mM, complete inhibition 33 1.1.1.284 Hg2+ NADH protects more effectively than NAD+ 33 1.1.1.284 Hg2+ 1 mM, 90% inhibition 33 1.1.1.284 imidazole 1 mM, 32% inhibition 470 1.1.1.284 iodoacetamide 1 mM, 20% inhibition 67 1.1.1.284 iodoacetate NADH protects more effectively than NAD+ 93 1.1.1.284 iodoacetate 1 mM, 15% inhibition 93 1.1.1.284 methyl methanethiosulfonate complete inactivation 2346 1.1.1.284 methylglyoxal - 322 1.1.1.284 additional information it is unclear whether activation or inhibition by fatty acids is of physiological importance 2 1.1.1.284 additional information not inhibited by 0.4 mM 2-aminodecanoic acid and 10 mM glutathionesulfonic acid 2 1.1.1.284 additional information GSNOR inhibitors may be novel tools for regulating nitric oxide bioactivity and assessing the role of S-nitrosothiols in vivo 2 1.1.1.284 additional information strong oxidizing agents are capable of reducing Arabidopsis thaliana GSNOR activity. Plant systems reversibly inhibit their GSNOR activity in response to oxidative radicals 2 1.1.1.284 additional information plant systems reversibly inhibit their GSNOR activity in response to oxidative radicals 2 1.1.1.284 additional information oxidative post-translationally modification of GSNOR inhibits the activity of the enzyme suggesting a direct crosstalk between reactive oxygen species (ROS)- and reactive nitrogen species (RNS)-signaling 2 1.1.1.284 additional information GSNOR enzymatic activity, but not gene expression, is inhibited by the nitrogen assimilatory pathway via post-transcriptional S-nitrosation, preventing any scavenging of GSNO 2 1.1.1.284 additional information lacking an S-nitrosyl or S-hydroxymethyl group that binds to the active site zinc atom, the affinity of inhibitors GSH and S-methylglutathione is reduced by 2-3 orders of magnitude compared to GSNO and HMGSH 2 1.1.1.284 additional information not inhibitory: N-ethylmaleimide 2 1.1.1.284 N6022 noncompetitive 42551 1.1.1.284 N6022 - 42551 1.1.1.284 N6022 a GSNOR inhibitor 42551 1.1.1.284 N6022 a pyrolle-based compound, that is a significantly stronger noncompetitive inhibitor compared to fatty acids, inhibiting SlGSNOR at nanomolar concentrations 42551 1.1.1.284 NAD+ competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration 7 1.1.1.284 NAD+ inhibits reverse reaction with NAD+ or NADP+ 7 1.1.1.284 NAD+ product inhibition 7 1.1.1.284 NADH competitive inhibition, product inhibition 8 1.1.1.284 NADH - 8 1.1.1.284 NADH competitive with NAD+ 8 1.1.1.284 NADH inhibits reaction with formaldehyde, glutathione and NAD+ 8 1.1.1.284 NADH product inhibition 8 1.1.1.284 NADPH inhibits reaction with formaldehyde, glutathione and NAD+ 5 1.1.1.284 NEM 1 mM, 35% inhibition 89 1.1.1.284 nitroprusside GSNO reductase activity is about two- to threefold decreased following a 2h stress treatment with 1 mM sodium nitroprusside 16703 1.1.1.284 NO GSNOR1 activity decreases in response to NO donors 277 1.1.1.284 NO - 277 1.1.1.284 NO susceptibility of the enzymatic activity to NO donors in vitro and its subsequent restoration after treatment with reducing agent dithiothreitol (DTT) 277 1.1.1.284 NO2- 1 mM, 14% inhibition 852 1.1.1.284 Nonanoic acid pelargonic acid 15888 1.1.1.284 octanoic acid caprylic acid 1649 1.1.1.284 octanoic acid - 1649 1.1.1.284 octanoic acid noncompetitive inhibitor 1649 1.1.1.284 p-hydroxymercuribenzoate - 98 1.1.1.284 PCMB - 78 1.1.1.284 PCMB 1 mM, complete inhibition 78 1.1.1.284 peroxynitrite treatment of AtGSNOR with peroxynitrite, known as tyrosine nitrating agent, modifies this enzyme and inhibits its activity 1220 1.1.1.284 pyrazole - 1078 1.1.1.284 S-acetamidoglutathione - 77151 1.1.1.284 S-formylglutathione - 1656 1.1.1.284 S-formylglutathione inhibits reaction with formaldehyde, glutathione and NAD+ 1656 1.1.1.284 S-formylglutathione product inhibition with S-hydroxymethylglutathione 1656 1.1.1.284 S-hydroxymethylglutathione - 3095 1.1.1.284 S-Methylglutathione - 3363 1.1.1.284 S-Methylglutathione noncompetitive inhibitor, inhibits GSNO reduction 3363 1.1.1.284 Tridecanoic acid tridecylic acid 30483 1.1.1.284 Undecanoic acid undecylic acid, most efficient inhibitor 16009 1.1.1.284 Zn2+ 1 mM, 40% inhibition 14 1.1.1.284 Zn2+ 1 mM, 50% inhibition 14 1.1.1.284 Zn2+ - 14 1.1.1.284 Zn2+ 1 mM, 28% inhibition 14