2.3.3.14 enzyme is very unstable in crude and protamine sulphate-treated desalted extracts 488145 2.3.3.14 freezing and thawing results in 50% loss of activity 488145 2.3.3.14 the purified enzyme can be stabilized using a combination of guanidine hydrochloride, alpha-cyclodextrin and ammonium sulfate 660793