2.3.1.86 2-mercaptoethanol, inactivation during storage 487605 2.3.1.86 acylation of free protein amino groups leads to reversible dissociation into subunits 487602 2.3.1.86 low ionic strength leads to inactivation 487602 2.3.1.86 low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M 487611 2.3.1.86 PMSF ensures isolation of native enzyme 487607