2.7.1.6 dithiothreitol protects the enzyme better than 2-mercaptoethanol against denaturation on storage at 4°C 2.7.1.6 overnight dialysis at pH above 9.2 or below pH 5.5 results in loss of all enzymatic activity 2.7.1.6 the enzyme is stabilized during the purification procedure by the inclusion of 10% glycerol, 1 mM phosphate and 20 mM 2-mercaptoethanol in all buffer solutions