2.7.1.158	accumulation of soluble ZmIPK1 protein is very low under various growing temperatures and induction conditions, and the majority of the fusion protein is detected in inclusion bodies. Therefore, an alternative strategy using chaperone protein coexpression to enhance ZmIPK1 protein solubility is exploited.	
2.7.1.158	the first bound Zn2+ ion, Zn1, is necessary for protein folding and stability