2.6.1.43 freezing and thawing does not affect activity 640046 2.6.1.43 glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM 640045 2.6.1.43 proteolytic hydrolysis of the cytosolic isozyme generates an enzymatically more active form 640060 2.6.1.43 purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly 640053 2.6.1.43 repeated freezing and thawing causes inactivation 640062 2.6.1.43 repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol 640057 2.6.1.43 stability is improved by 10-15% glycerol 640050