2.3.1.86 2-mercaptoethanol, inactivation during storage 2.3.1.86 acylation of free protein amino groups leads to reversible dissociation into subunits 2.3.1.86 low ionic strength leads to inactivation 2.3.1.86 low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M 2.3.1.86 PMSF ensures isolation of native enzyme