3.2.1.133 2-mercaptoethanol about 120% activity at 1% (v/v) 732767 3.2.1.133 Ca2+ the enzyme is activated by 15% at 5 mM 710449 3.2.1.133 DMSO enzyme activity increases to 136% in the presence of 10% (v/v) DMSO 707248 3.2.1.133 EDTA increases activity of wild-type enzyme and of mutant enzyme R26Q/S169N/I333V/A398V/Q411L/P453L 665335 3.2.1.133 ethanol enzyme activity increases to 113% in the presence of 10% (v/v) ethanol 707248 3.2.1.133 methanol enzyme activity increases to 104.4% in the presence of 10% (v/v) methanol 707248 3.2.1.133 Mn2+ the enzyme is activated by 51% at 5 mM 710449 3.2.1.133 Tween-20 0.1% (v/v), stimulates 750727