3.1.1.74	16-hydroxyhexadecanoic acid	is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid	691941	
3.1.1.74	DMF	activates 10% at 40% v/v	729000	
3.1.1.74	DMF	activates 6% at 40% v/v	729000	
3.1.1.74	DMSO	activates 14% at 40% v/v	729000	
3.1.1.74	DMSO	activates 9% at 40% v/v	729000	
3.1.1.74	additional information	absence of interfacial activation	208329	
3.1.1.74	additional information	no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket	709196	
3.1.1.74	additional information	Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase	710477	
3.1.1.74	additional information	pseudo-activation in presence of sodium bis(2-ethylhexyl)ester sulfosuccinic acid and hexadecyltrimethyl-ammoniumbromide	208328	
3.1.1.74	additional information	water:surfactant molar rate has a marked influence on the enzyme activity, with the best results in the range between 5 and 8. The use of detergents improves the reaction yield during wetting of cotton fibers. Increase in the stereoselectivity of the primary hydroxyl group acylation is obtained through the preincubation of the enzyme in the presence of the substrate diol 1, there is no correlation with the incubation time	707009