3.4.21.B57	guanidinium hydrochloride	90% activation at 4 M	
3.4.21.B57	additional information	destabilization of the hydrophobic core of Tk-propeptide by a nonpolar-to-polar amino acid substitution is an effective way to increase the activation rate of Pro-Tk-subtilisin	
3.4.21.B57	additional information	the activities in the presence of 1% SANISOL C and AMPHITOL 20 N are higher than those at the concentration of 0.1%. This may relate to the critical micelle concentration of the surfactants. Improvement of the enzyme activity in the presence of nonionic surfactants might be due to the stimulation of conformational changes in Tk-SP or the substrate, leading to activity gain	
3.4.21.B57	Urea	slight activation at 1-4 M