3.1.1.74	16-hydroxyhexadecanoic acid	is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid	691941	
3.1.1.74	DMF	activates 10% at 40% v/v	729000	
3.1.1.74	DMF	activates 6% at 40% v/v	729000	
3.1.1.74	DMSO	activates 14% at 40% v/v	729000	
3.1.1.74	DMSO	activates 9% at 40% v/v	729000	
3.1.1.74	additional information	absence of interfacial activation	208329	
3.1.1.74	additional information	no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket	709196	
3.1.1.74	additional information	Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase	710477	
3.1.1.74	additional information	pseudo-activation in presence of sodium bis(2-ethylhexyl)ester sulfosuccinic acid and hexadecyltrimethyl-ammoniumbromide	208328	
3.1.1.74	additional information	water:surfactant molar rate has a marked influence on the enzyme activity, with the best results in the range between 5 and 8. The use of detergents improves the reaction yield during wetting of cotton fibers. Increase in the stereoselectivity of the primary hydroxyl group acylation is obtained through the preincubation of the enzyme in the presence of the substrate diol 1, there is no correlation with the incubation time	707009	
3.1.1.74	N,N-diethyl-2-phenylacetamide	increases hydrolysis rates of semi-crystalline poly(ethylene terephthalate) films and fabrics for cutinase. The outer layers of the polymer will be better exposed to the enzyme in the presence of N,N-diethyl-2-phenylacetamide. Overall enhancement in colour depth of 300%	709196	
3.1.1.74	olive oil	Thcut1 mRNA is strongly induced in vitro by olive oil	691941	
3.1.1.74	SDS	5%, 131% of initial activity	750134	
3.1.1.74	sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate	activates 10% at 10 mM	729000	
3.1.1.74	sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate	activates 24% at 10 mM	729000	
3.1.1.74	sodium dioctyl sulfosuccinate	in the absence of surfactant the S54D variant catalytic activity is similar to that of the wild type cutinase, whereas L153Q and T179C variants show a lower activity	691090	
3.1.1.74	Triton X-100	5%, 190% of initial activity	750134	
3.1.1.74	Tween 20	5%, 118% of initial activity	750134	
3.1.1.74	Tween 40	5%, 139.7% of initial activity	750134	
3.1.1.74	Tween 60	5%, 139.5% of initial activity	750134	
3.1.1.74	Tween 80	5%, 141.9% of initial activity	750134