2.3.1.8 1,3-dimercaptopropanol activates at 0.01 M 487515 2.3.1.8 cysteine activates at 0.01 M 487515 2.3.1.8 dithiothreitol maxmimum activity in the presence of 1 mM dithiothreitol 736033 2.3.1.8 hydrogen sulfide activates at 0.01 M 487515 2.3.1.8 additional information not significant efects: NADH, ATP, phosphoenol pyruvate and aspartate, even at concentrations as high as 10 mM 720217 2.3.1.8 NH4Cl 3fold stimulation at 40 mM. No significant increase in activity above 40 mM 674792 2.3.1.8 NH4Cl maximal stimulation at 40 mM, 3fold 674792 2.3.1.8 PPIB cytoplasmic cytophilin, interaction with phosphate acetyltransferase leads to enhanced activity and alteration in Km value. PPIase activity is not essential for these interactions, as PPIB F99A active site mutant still interacts with phosphate acetyltransferase, but PPIB activity is responsible for the observed phosphate acetyltransferase activity enhancement 720425 2.3.1.8 pyruvate - 487532 2.3.1.8 pyruvate 156% of activation at 5 mM 720217 2.3.1.8 pyruvate isoform PtaII has 133% activity in the presence of 0.05 mM pyruvate 736033 2.3.1.8 pyruvate stimulates activity of wild-type enzyme 0.2fold over control, and the activity of the mutant enzyme R252H 3fold over control 674792 2.3.1.8 pyruvate stimulates wild-type activity, its effect is potentiated in the variants, being most pronounced on R252H 674792 2.3.1.8 thiolglycolic acid activates at 0.01 M 487515