2.7.1.73 K+ increases activity, optimum concentration: 40 mM 2.7.1.73 K+ the enzyme requires both Mg2+ and K+ 2.7.1.73 Mg2+ reaction is dependent on presence of divalent cation 2.7.1.73 Mg2+ enzyme requires both Mg2+ and K+ 2.7.1.73 Mg2+ required, optimal concentration: 5 mM 2.7.1.73 Mn2+ can partially replace Mg2+ in activation