EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.250 | analysis | the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis | Candida tropicalis |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.11 | Mg2+ stabilizes | Candida tropicalis |
1.1.1.250 | Mg2+ enhances the stability | Candida tropicalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.11 | 0.12 | - |
NAD+ | - |
Candida tropicalis | |
1.1.1.11 | 39.8 | - |
D-arabinitol | - |
Candida tropicalis | |
1.1.1.250 | 0.12 | - |
NAD+ | - |
Candida tropicalis | |
1.1.1.250 | 39.8 | - |
D-arabinitol | - |
Candida tropicalis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.11 | Mg2+ | 1-5 mM increases activity about 50%, also enhances stability | Candida tropicalis | |
1.1.1.250 | Mg2+ | 1-5 mM, 50% increase of activity | Candida tropicalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.11 | 31000 | - |
x * 31000, SDS-PAGE | Candida tropicalis |
1.1.1.250 | 31000 | - |
x * 31000, SDS-PAGE | Candida tropicalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.11 | D-arabinitol + NAD+ | Candida tropicalis | - |
D-ribulose + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.11 | Candida tropicalis | - |
- |
- |
1.1.1.250 | Candida tropicalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.250 | - |
Candida tropicalis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.250 | D-arabinitol + NAD+ = D-ribulose + NADH + H+ | in the reverse reaction the enzyme catalyzes transfer of 4(S) hydrogen of NADH | Candida tropicalis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.11 | 96 | - |
- |
Candida tropicalis |
1.1.1.250 | 96 | - |
- |
Candida tropicalis |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.250 | -80°C, stable | Candida tropicalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.11 | D-arabinitol + NAD+ | - |
Candida tropicalis | D-ribulose + NADH + H+ | - |
? | |
1.1.1.11 | Galactitol + NAD+ | 1.6% activity | Candida tropicalis | ? | - |
? | |
1.1.1.11 | L-Xylulose + NADH | 3% activity | Candida tropicalis | ? | - |
? | |
1.1.1.11 | additional information | no activity with D-mannitol | Candida tropicalis | ? | - |
? | |
1.1.1.11 | Xylitol + NAD+ | 5% activity | Candida tropicalis | ? | - |
? | |
1.1.1.250 | D-arabinitol + NAD+ | r | Candida tropicalis | D-ribulose + NADH + H+ | - |
? | |
1.1.1.250 | D-xylulose + NADH | 1% of the activity with D-ribulose | Candida tropicalis | ? | - |
? | |
1.1.1.250 | Galactitol + NAD+ | 1.6% of the activity with D-arabinitol | Candida tropicalis | ? | - |
? | |
1.1.1.250 | L-Xylulose + NADH | 3% of the activity with D-ribulose | Candida tropicalis | ? | - |
? | |
1.1.1.250 | Xylitol + NAD+ | 5% of the activity with D-arabinitol | Candida tropicalis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.11 | ? | x * 31000, SDS-PAGE | Candida tropicalis |
1.1.1.250 | ? | x * 31000, SDS-PAGE | Candida tropicalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.11 | 6.5 | - |
optimal activity, reduction | Candida tropicalis |
1.1.1.250 | 10 | 11 | oxidation of D-arabinitol | Candida tropicalis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.11 | 10 | 11 | enzyme activity increases with increasing pH, measurements above pH 11.0 not feasible | Candida tropicalis |
1.1.1.250 | 8 | 11 | pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity | Candida tropicalis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.11 | 6 | 9.5 | more labile at increasing pH from 6.0-9.5 | Candida tropicalis |
1.1.1.250 | 11 | - |
unstable above | Candida tropicalis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.11 | NAD+ | - |
Candida tropicalis | |
1.1.1.11 | NADH | - |
Candida tropicalis | |
1.1.1.250 | NAD+ | - |
Candida tropicalis | |
1.1.1.250 | NADH | - |
Candida tropicalis |