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Literature summary extracted from
- Purification and characterization of salutaridine:NADPH 7-oxidoreductase from Papaver somniferum (1993), Phytochemistry, 34, 125-132.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.248 | 0.018 | - |
salutaridinol | - |
Papaver somniferum |  |
| 1.1.1.248 | 0.023 | - |
salutaridine | - |
Papaver somniferum | |
| 1.1.1.248 | 0.055 | - |
NADP+ | - |
Papaver somniferum | |
| 1.1.1.248 | 0.125 | - |
NADPH | - |
Papaver somniferum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.248 | cytosol | - |
Papaver somniferum | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | 52000 | - |
gel filtration | Papaver somniferum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.248 | salutaridine + NADPH + H+ | Papaver somniferum | formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids | salutaridinol + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.248 | no activity in Papaver alpinum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver atlanticum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver dubium | - |
- |
- |
| 1.1.1.248 | no activity in Papaver feddei | - |
- |
- |
| 1.1.1.248 | no activity in Papaver lateritium | - |
- |
- |
| 1.1.1.248 | no activity in Papaver oreophilum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver orientale | - |
- |
- |
| 1.1.1.248 | no activity in Papaver persicum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver pilosum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver rhoeas | - |
- |
- |
| 1.1.1.248 | no activity in Papaver rupifragum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver strigosum | - |
- |
- |
| 1.1.1.248 | no activity in Papaver tauricula | - |
- |
- |
| 1.1.1.248 | no activity in Papaver triniifolium | - |
- |
- |
| 1.1.1.248 | Papaver bracteatum | - |
- |
- |
| 1.1.1.248 | Papaver somniferum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.248 | - |
Papaver somniferum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.248 | salutaridinol + NADP+ = salutaridine + NADPH + H+ | highly substrate specific transfer of the pro-S hydride, B-type, of NADPH to C-7 of salutaridine | Papaver somniferum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.248 | cell suspension culture | - |
Papaver somniferum | - |
| 1.1.1.248 | additional information | no activity in leaves | Papaver somniferum | - |
| 1.1.1.248 | plant capsule | - |
Papaver somniferum | - |
| 1.1.1.248 | root | - |
Papaver somniferum | - |
| 1.1.1.248 | seedling | - |
Papaver somniferum | - |
| 1.1.1.248 | shoot | - |
Papaver somniferum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | additional information | - |
- |
Papaver somniferum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.248 | 4°C, pH 7.0-9.0, 0.6 M KCl, half-life: 30 days | Papaver somniferum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.248 | salutaridine + NADPH + H+ | r | Papaver somniferum | (7S)-salutaridinol + NADP+ | - |
? | |
| 1.1.1.248 | salutaridine + NADPH + H+ | formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids | Papaver somniferum | salutaridinol + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.248 | monomer | 1 * 52000, SDS-PAGE | Papaver somniferum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | 30 | 35 | both directions | Papaver somniferum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | 6 | 6.5 | formation of (7S)-salutaridinol | Papaver somniferum |
| 1.1.1.248 | 9 | 9.5 | formation of salutaridine | Papaver somniferum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | 5.8 | 7.8 | 50% of maximal activity at pH 5.8 and at pH 7.8, formation of (7S)-salutaridinol | Papaver somniferum |
| 1.1.1.248 | 8.5 | 10 | 50% of maximal activity at pH 5.8 and at pH 7.8, formationof salutaridine | Papaver somniferum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.248 | 7 | 9 | 4°C, half-life: 30 days | Papaver somniferum |
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Release 2023.1 (January 2023)
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