EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.19.3 | overexpression in Escherichia coli | Streptococcus pyogenes |
EC Number | General Stability | Organism |
---|---|---|
3.4.19.3 | freezing and thawing, 67% of the activity is recovered after one cycle, 70% loss of activity after 5 cycles | Streptococcus pyogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.3 | 2-Pyrrolidone | competitive | Streptococcus pyogenes | |
3.4.19.3 | Co2+ | - |
Streptococcus pyogenes | |
3.4.19.3 | Hg2+ | - |
Streptococcus pyogenes | |
3.4.19.3 | iodoacetamide | - |
Streptococcus pyogenes | |
3.4.19.3 | iodoacetate | - |
Streptococcus pyogenes | |
3.4.19.3 | Zn2+ | - |
Streptococcus pyogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.3 | 1.79 | - |
Pyroglutamyl-beta-naphthylamide | - |
Streptococcus pyogenes | |
3.4.19.3 | 20 | - |
pyroglutamyl-p-nitroanilide | - |
Streptococcus pyogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 23500 | - |
4 * 23500, SDS-PAGE | Streptococcus pyogenes |
3.4.19.3 | 85000 | - |
nondenaturing PAGE | Streptococcus pyogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.19.3 | Streptococcus pyogenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.19.3 | - |
Streptococcus pyogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 20.8 | - |
- |
Streptococcus pyogenes |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.19.3 | 4°C, almost 60% of the initial activity is recovered after 1 month | Streptococcus pyogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.19.3 | pyroglutamyl-beta-naphthylamide + H2O | - |
Streptococcus pyogenes | pyroglutamate + beta-naphthylamine | - |
? | |
3.4.19.3 | pyroglutamyl-p-nitroanilide + H2O | - |
Streptococcus pyogenes | pyroglutamate + p-nitroaniline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.19.3 | tetramer | 4 * 23500, SDS-PAGE | Streptococcus pyogenes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 22 | - |
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month | Streptococcus pyogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 7 | - |
- |
Streptococcus pyogenes |